Hygromycin B
Hygromycin B resistance mediates elimination of Leishmania virus from persistently infected parasites.
... Loss of virus during growth in medium containing hygro- mycin B. To distinguish whether drug selection cures viral infection or simply reduces the virus copy number, parasite cultures were transferred into fresh ...
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Use of Coniothyrium minitans transformed with the hygromycin B resistance gene to study survival and infection of Sclerotinia sclerotiorum sclerotia in soil
... (+/x hygromycin B) and ...on hygromycin B- amended agar discs were omitted from the analysis of percentage sclerotial viability, and comparisons were noted at the 5 % significance ...
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Co-transformation of the sclerotial mycoparasite Coniothyrium minitans with hygromycin B resistance and [beta]-glucuronidase markers
... Southern analysis showed that neither the PCR-derived hph-gene probe nor the uidA-gene probe hybridized to digested DNA from wild type A69 (Fig. 1). Both probes did hybridize to one or more fragments in digested DNA of ...
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Feline Immunodeficiency Virus OrfA Is Distinct from Other Lentivirus Transactivators
... Generation of a stable cell line expressing OrfA. Despite having a functional antibody that recognizes the OrfA protein in Western blot analyses, as well as in vitro-generated OrfA, we failed to detect OrfA in infected ...
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Improved self-inactivating retroviral vectors derived from spleen necrosis virus.
... U3-deleted vectors carrying the hygromycin B phosphotransferase gene did not observably undergo LTR reconstitution, even when replicated in helper cells containing SNV-LTR sequences.. Ho[r] ...
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PRODUCTION OF TRANSGENIC INDICA RICE (ORYZA SATIVA L.) CV. MR 81 VIA PARTICLE BOMBARDMENT SYSTEM
... of hygromycin B were effective in selecting the transformants (data not shown), we have used this concentration in the subsequent ...50mg/L hygromycin B was strong enough to inhibit further ...
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Genetic transformation of Knufia Petricola A95 - a model organism for biofilm-material interactions
... Colonies were visible on hygromycin B containing MEAS agar two to three weeks after transformation of A95 protoplasts with pCB1004 (Figure 3A). Five ran- domly selected, transformed colonies were able to ...
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Expression of Hygromycin Phosphotransferase Alters Virulence of Histoplasma capsulatum
... of hygromycin B concentrations of ⬎ 300 g/ ml, while UC18 and UC19 were unable to grow in the presence of ⬎ 150 g/ml of hygromycin ...demonstrated hygromycin B phosphotransferase ...
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Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation
... the hygromycin phosphotransferase (hph) gene utilized for positive selection of ...and hygromycin B) and samples were taken for hyphal section visualization by confocal ...
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Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas
... spectinomycin, hygromycin B, kanamycin and ...paromomycin, hygromycin B and zeocin resistance genes are commonly used as selectable mark- ers ...
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PEG mediated and Agrobacterium mediated transformation in the mycopathogen Verticillium fungicola
... of hygromycin-B resistant, GFP-expressing and GUS-expressing isolates will allow selective isolation, quantification and in vivo tracking of the transformants from, and in mushrooms and compost, especially ...
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Identification of candidate genes with pro-apoptotic properties by functional screening of randomly fragmented C DNA libraries
... Identification of pro-apoptotic genes using randomly fragmented cDNA libraries: the randomly fragmented cdna libraries were introduced into a375, Hs892-t, cls-354, HepG2, acHn and Mcf-7 cells by the nu- cleofector ...
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DevA, a GntR-like transcriptional regulator required for development in streptomyces coelicolor
... coli into Streptomyces Plasmid integrating at phage C31 attB site, carrying apramycin resistance apr Plasmid integrating at phage BT1 attB site, carrying hygromycin resistance hyg pSET[r] ...
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Aumann, Dorothea (2017): Generierung genetisch modifizierter Hühnermodelle für die immunologische Forschung. Dissertation, LMU München: Tierärztliche Fakultät
... Zur Generierung transgener Hühner mit ubiquitärer mCherry Expression wurde ein Konstrukt kloniert, in dem das mCherry-Gen unter einem Hühner-b-Aktin-Promoter exprimiert wird. Zur Selektion erfolgreich ...
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Female reproductive system of Amaranthus as the target for Agrobacterium mediated transformation
... The pDRB6b vector digested with HindIII/KpnI served as the backbone vector for p5b5, p5f7 and p5d9 recom- binant vectors. The recombinant vector was constructed in between RB2 and LB of pDRB6b. p5b5 contained Ubi1P ...
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On Locally b#-Closed Sets and Weakly b#-Closed Sets
... Now to prove (iii).Let f: (X, ) (Y, ) be completely continuous. Then by Definition 2.3(ii), the inverse image of every open set in Y is regular open in X. Let V be open in Y. Then f -1 (V) is regular open in X. Using the ...
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Transfer of a Supernumerary Chromosome Between Vegetatively Incompatible Biotypes of the Fungus Colletotrichum gloeosporioides
... genetic recombination between biotypes A and B of Vegetative incompatibility in fungi is conditioned by C. gloeosporioides that infect the legumes Stylosanthes in the interaction of alleles at het loci in the ...
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WRITING AN ABSTRACT
... First, Simon uses statistics to point out that drunk driving is a common crime in the United States, and because of that fact, judges and jurors alike are likely to be biased in favor of[r] ...
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ShootinganElephanttext
... The older men said I was right, the younger men said it was a damn shame to shoot an elephant for killing a coolie, because an elephant was worth more than any damn Coringhee coolie. An[r] ...
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Irish, Scottish, Welsh assignment
... 'Welsh is an official British language, the oldest language in Europe and the most common in Britain after English.' Photograph: David Hunter/Robert Harding /REX.. Last weekend I took [r] ...
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