Identification of Open Reading Frames (ORFs)
Identification of proteins encoded by the L1 and L2 open reading frames of human papillomavirus 1a.
... blotting, antiserum prepared against the Li (pLl-776) fusion protein detected the major 57,000-molecular-weight HPV-1 structural protein (57K protein) consistently in extracts of warts i[r] ...
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The characterization of peptides expressed from short open reading frames (sORFs)
... In this project, 1,879,681 MS/MS spectra were obtained in my experiments. The MS/MS data matched in human RNA database were examined to confirm that they met the criteria for a sPEP. Those MS/MS spectra that match main ...
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Extensive translation of small Open Reading Frames revealed by Poly-Ribo-Seq
... ncORFs identification We identified uORFs and ncORFs longer than 10 aa with an AUG start codon followed by an in-frame stop codon within the annotated 5′-UTRs and ncRNA transcripts, using the emboss getorf ...
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Extensive translation of small Open Reading Frames revealed by Poly-Ribo-Seq
... ncORFs identification We identified uORFs and ncORFs longer than 10 aa with an AUG start codon followed by an in-frame stop codon within the annotated 5′-UTRs and ncRNA transcripts, using the emboss getorf ...
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Identification, cloning, and sequencing of a fragment of Amsacta moorei entomopoxvirus DNA containing the spheroidin gene and three vaccinia virus-related open reading frames.
... the EcoRI-D fragment of AmEPV DNA. Primer extension analysis of the spheroidin transcript. 4) located 65 bp downstream of the initiating methionine codon found in the TAAATG motif at the[r] ...
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Evidence for conservation and selection of upstream open reading frames suggests probable encoding of bioactive peptides
... Identification of conserved and orthologs ORFs To identify ORFs conserved between human and mouse, we performed an NCBI blastp alignment of peptide trans- lations of the mouse ORF sets against the equivalent human ...
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Small Open Reading Frames, Non-Coding RNAs and Repetitive Elements in Bradyrhizobium japonicum USDA 110
... Conclusions We analyzed the length of 1391 ORFs that were recently added to the re-annotated genome of the soybean symbiont B. japonicum USDA 110 and found that 1080 of them are sORFs, show- ing that mostly small genes ...
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Herpes Simplex Virus 1 Open Reading Frames O and P Are Not Necessary for Establishment of Latent Infection in Mice
... DISCUSSION A unique property of HSV is that it carries a large number of accessory genes designed in large part to control both the intracellular and extracellular environment in which it repli- cates. Latency is a ...
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Identification of endogenous retroviral reading frames in the human genome
... The vast majority of the mapped HERV regions contain several frame-shift mutations or in-frame stop codons that truncate the viral ORFs and thus testify to their old associ- ation with the human genome. In fact, we ...
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Characterization of Baculovirus Repeated Open Reading Frames (bro) in Bombyx moriNucleopolyhedrovirus
... 1) was cotransfected into BmN cells with BmBroAD to con- struct a double-deletion mutant. Since the bro-c region would be replaced with the -galactosidase gene in this plasmid, the final recombinant was selected by the ...
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Gene expression regulation by upstream open reading frames in rare diseases
... Belonging to the set of rare diseases associated with by the time the ribosome reaches the initiation codon of the main ORF 22 . The advent of RiboSeq brought new insights about translational regulation, allowing the ...
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Molecular characterization of partial open reading frames 1a and 2 of the human astroviruses in South Korea
... 24. Noel JS, Lee TW, Kurtz JB, Glass RI, Monroe SS: Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing. J Clin Microbiol 1995, 33:797-801. 25. Kang YH, Park YK, Ahn JB, ...
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Human Herpesvirus 7 Open Reading Frames U12 and U51 Encode Functional β-Chemokine Receptors
... The identification of ELC, SLC, TARC, and MDC as specific ligands for U12 and U51 now enables us to examine the possible roles of U12 and U51 in the infection process and life cycle of ...
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RNA G quadruplexes at upstream open reading frames cause DHX36 and DHX9 dependent translation of human mRNAs
... upstream open reading frames ...Transcriptome-wide identification of DHX9 binding sites shows that reduced translation is mediated through direct physical interaction between the helicase and ...
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Classification and function of small open reading frames
... Table 1 – Properties of smORFs in Fruit Flies and Mammals. Animal genomes contain millions of open reading frames (ORFs) between an ATG (start) and a stop codon. Most of these are found in ...
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Mutational analysis of the open reading frames in the transposable element IS1.
... TO rule o u t effects on gene expression or cointegrate formation not directly related to the amber codon, a neutral mutation was made which leaves both insA an[r] ...
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Hundreds of putatively functional small open reading frames in Drosophila
... Abstract Background: The relationship between DNA sequence and encoded information is still an unsolved puzzle. The number of protein-coding genes in higher eukaryotes identified by genome projects is lower than was ...
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Translational Control via Protein-Regulated Upstream Open Reading Frames
... Analysis of the regulation of msl-2 mRNA by Sex lethal (SXL), which is critical for dosage compensa- tion in Drosophila, has uncovered a mode of trans- lational control based on common 5 0 untranslated region elements, ...
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Gene expression regulation by upstream open reading frames and human disease
... 1 Departamento de Gene´tica Humana, Instituto Nacional de Sau´de Dr. Ricardo Jorge, Lisboa, Portugal, 2 Center for Biodiversity, Functional and Integrative Genomics, Faculdade de Cieˆncias, Universidade de Lisboa, ...
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The Role of Upstream Open Reading Frames in Regulating Neuronal Protein Synthesis
... complexes are limiting, this may lead to the bypassing of the 0- and +1 frame start sites by scanning ribosomes, leading to more downstream initiation at the AUG of FMRP. This proposed mechanism can also bring the +2 RAN ...
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