immunofluorescence staining
Diagnosis of Gastric Syphilis by Direct Immunofluorescence Staining and Real Time PCR Testing
... immunofluorescent staining for pathogenic treponemes im- proved when Ito et ...and immunofluorescence staining, and a properly controlled PCR would seem to be an appropriate step in making a ...
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Automated PGP9 5 immunofluorescence staining: a valuable tool in the assessment of small fiber neuropathy?
... diagnostically efficient at distinguishing polyneuropa- thy patients [including small fiber neuropathy (SFN)] from normal subjects as controls. Skin biopsy immu- nostaining can help to detect aberrations in somatic ...
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Early detection of chlamydial inclusions combining the use of cycloheximide treated McCoy cells and immunofluorescence staining
... Experiments were designed i to determine the relative sensitivity of CY-treated and IR McCoy cells for the isolation of chlamydiae, ii to compare the efficiency of Giemsa and IF staining[r] ...
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Specific immunofluorescence staining of Treponema pallidum in smears and tissues
... pallidum in chancre smears 15 can easily be stained with specific fluorescein-labeled anti- T.pallidum conjugate absorbed with Treponema phagedenis.. However, this conjugate has not been[r] ...
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Rapid detection of bacteria in cerebrospinal fluid by immunofluorescence staining on membrane filters
... The sensitivity of this procedure was determined and compared with that of the slide Gram stain of centrifuged samples, latex agglutination, and a previously developed filter Gram stain.[r] ...
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Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae
... Members of the genus Rickettsia are Gram-negative strictly intracellular Alphaproteobacteria, multiplying by binary fission in the cytoplasm and nuclei of infected host cells [13, 19]. Rickettsiae are arthropod-vectored ...
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Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
... study immunofluorescence staining and Western blotting were used to detect the expression of autophagy markers (LC3II and beclin1) in the brains of neonatal mice with hypoxic-ischemic brain injuries after ...
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Nonrandom Segregation of the Mouse Univalent X Chromosome: Evidence of Spindle-Mediated Meiotic Drive
... 2.—Combined immunofluorescence and FISH anal- selection (Kaufman 1972; Luthardt 1976; Brook 1983; ysis of oocytes from XO ...c) Immunofluorescence staining ...
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RIP140 increases APC expression and controls intestinal homeostasis and tumorigenesis
... performed immunofluorescence staining to detect total and active (ABC) β-catenin in the two HCT116 cell lines, with some of the cells overexpressing ...ABC staining in cells overexpressing RIP140 ...
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Environmental Isolation of Balamuthia mandrillaris Associated with a Case of Amebic Encephalitis
... indirect immunofluorescence staining with rabbit anti-Balamuthia serum and human anti- Balamuthia antibody-containing serum from the amebic encephalitis ...
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Differentiation of human epidermis derived mesenchymal stem cell like pluripotent cells into neural like cells in culture and after transplantation
... Skin is the largest organ of the human body and a possible source of stem cells for research and cell-based therapy. We have isolated a popula- tion of mesenchymal stem cell-like pluripotent cells from human epidermis, ...
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Rickettsiae and Borrelia burgdorferi in ixodid ticks
... Nymphs and adults of hard-bodied ticks were collected in Connecticut and tested by direct and indirect immunofluorescence staining methods for rickettsiae and Borrelia burgdorferi.. Of t[r] ...
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Bright Polymer Dots Tracking Stem Cell Engraftment and Migration to Injured Mouse Liver
... As shown by the typical observations from immunofluorescence staining (Figure 6C), overlapping of the fluorescence images showed no sign of colocalization of the Pdot-labeled MSCs (Re[r] ...
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Temporal Changes in Outer Surface Proteins A and C of the Lyme Disease Associated Spirochete, Borrelia burgdorferi, during the Chain of Infection in Ticks and Mice
... down-regulation of OspA in infected ticks during feeding (11) and the apparent lack of OspA on spirochetes having dissem- inated from the midgut to the tick’s salivary glands (11). Obonyo et al. (28) recently ...
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Evaluation of oral keratinocyte progenitor and T-lymphocite cells response during early healing after augmentation of keratinized gingiva with a 3D collagen matrix - a pilot study
... Following immunofluorescence staining with different stem- and differentiation markers, we concluded that after 7 days and 14 days the majority of cells that repopulate the matrix were actively ...
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Myocardial localization and isoforms of neural cell adhesion molecule (N CAM) in the developing and transplanted human heart
... involved in cardiac morphogenesis and innervation. Immunohistochemical techniques and Western blot analysis were used to determine the localization and isoforms of N-CAM in the developing and extrinsically denervated ...
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Effects of the tom1 mutation of Arabidopsis thaliana on the multiplication of tobacco mosaic virus RNA in protoplasts.
... In PD114 protoplasts inoculated with TMV-Cg RNA, the percentage of infected TMV-Cg positive by immunofluorescence staining cells was low and the accumulation of positive-strand RNAs and [r] ...
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Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver.
... The B19 virus antigen was detected on about 5% of cells in the culture by immunofluorescence staining, and the stained cells were identified as erythroid lineage cells by double staining[r] ...
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The random inactivation of the X chromosome carrying the defective gene responsible for X linked hyper IgM syndrome (X HIM) in female carriers of HIGM1
... We investigated the inactivation of the defective X chromosome in these female carriers by immunofluorescence staining, to identify the fraction of in vitro activated T cells expressing [r] ...
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Fasudil Attenuates LPS-Induced Acute Lung Injury by Preventing Endothelial Dysfunction
... H&E staining showed severe inflammation in LPS group which was mitigated by fasudil ...immunohistochemistry staining showed fasudil remarkably reduced neutrophil infiltration into lung interstitium and ...
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