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Immunohistochemical Staining

Value of p53 and estrogen receptors immunohistochemical staining in endometrial carcinoma

Value of p53 and estrogen receptors immunohistochemical staining in endometrial carcinoma

... For the IHC staining, individual preparations are fixed in paraffin and cut at 3µm. After deparaffinization, tissue was embedded in methanol with 0.3% hydroperoxyl acid for 30 minutes at room temperature, in order ...

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Quantitative comparison of immunohistochemical staining measured by digital image analysis versus pathologist visual scoring

Quantitative comparison of immunohistochemical staining measured by digital image analysis versus pathologist visual scoring

... IHC staining within pathologist annotated and software-classified areas of carcinoma were compared for each ...IHC staining were used: the percentage of carcinoma with S100A1 staining (%Pos), and the ...

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A comparative study of AgNOR with pcna immunohistochemical staining in oral squamous cell carcinoma

A comparative study of AgNOR with pcna immunohistochemical staining in oral squamous cell carcinoma

... Study consisted of previously diagnosed 30 samples of oral squamous cell carcinomas. 17 samples were of well differentiated, 12 were of moderately differentiated and 1 poorly differentiated cases. Three sections were ...

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Image Analysis of HER2 Immunohistochemical Staining of Surgical Breast Cancer Specimens

Image Analysis of HER2 Immunohistochemical Staining of Surgical Breast Cancer Specimens

... prior to using an image analysis system as part of routine test- ing, there are several important steps to consider, including ensuring consistent staining quality of IHC slides, providing a detailed protocol for ...

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Complete antithrombin deficiency in mice results in embryonic lethality

Complete antithrombin deficiency in mice results in embryonic lethality

... by immunohistochemical staining might be present in several other organs, the myocardium and liver seemed to be more sus- ceptible than other tissues to fibrin(ogen) deposi- tion in antithrombin ...

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Inhibitor of IGF1 receptor alleviates the inflammation process in the diabetic kidney mouse model without activating SOCS2

Inhibitor of IGF1 receptor alleviates the inflammation process in the diabetic kidney mouse model without activating SOCS2

... DKD model. The results showed that blood glucose levels decreased with Ins, but not with the IGF1R inhibitor or Ben (Figure 1A). The urinary albumin excretion rate decreased significantly in all interventions, with the ...

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Gastric Mucosal Changes and Ghrelin Expression and Their Relation to Weight Reduction After Sleeve Gastrectomy

Gastric Mucosal Changes and Ghrelin Expression and Their Relation to Weight Reduction After Sleeve Gastrectomy

... LSG. Immunohistochemical staining of cellular ghrelin was used to evaluate the number and distribution of ghrelin producing cells in the resected specimen and the mucosal changes in the remaining gastric ...

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Pericyte targeting prodrug overcomes tumor resistance to vascular disrupting agents

Pericyte targeting prodrug overcomes tumor resistance to vascular disrupting agents

... immunofluorescence staining and 1:3,000 dilution for West- ern blotting) ...and immunohistochemical staining) were obtained from R&D ...cal staining), rabbit anti–β-tubulin (catalog 2128, ...

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Changes in proinflammatory cytokines and white matter in chronically stressed rats

Changes in proinflammatory cytokines and white matter in chronically stressed rats

... nohistochemical staining to localize and measure the expres- sion of proinflammatory cytokines and evaluate the status of white matter in different brain ...used immunohistochemical staining rather ...

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Reduced Skin Blistering in Experimental Epidermolysis Bullosa Acquisita After Anti-TNF Treatment

Reduced Skin Blistering in Experimental Epidermolysis Bullosa Acquisita After Anti-TNF Treatment

... addition, immunohistochemical staining demonstrated reduced numbers of macrophages in lesional skin in mice treated with TNF inhibitors compared with ...

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Original Article Spatiotemporal expression of BMP7 in the development of anorectal malformations in fetal rats

Original Article Spatiotemporal expression of BMP7 in the development of anorectal malformations in fetal rats

... Mature Wistar rats (body weight, 250-300 g) were provided by the Medical Animal Center, Shengjing Hospital of the China Medical University (Shenyang, PR China). Ethical approv- al was obtained from the China Medical ...

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Adenovirus-mediated gene transfer into normal rabbit arteries results in prolonged vascular cell activation, inflammation, and neointimal hyperplasia

Adenovirus-mediated gene transfer into normal rabbit arteries results in prolonged vascular cell activation, inflammation, and neointimal hyperplasia

... To test the hypothesis that adenovirus-mediated gene transfer results in inflammation and activation of the cells of the arterial wall, we performed immunohistochemical staining for ICAM[r] ...

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Original Article The HMG-CoA reductase inhibitor rosuvastatin promotes complement factor H expression in apolipoprotein E-knockout mice

Original Article The HMG-CoA reductase inhibitor rosuvastatin promotes complement factor H expression in apolipoprotein E-knockout mice

... expression. Immunohistochemical staining showed that CFH was expressed in group A, and this was accompanied by increased mRNA and protein levels and the severity of ...

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Magnetization Transfer Micro MR Imaging of Live Excised Lamprey Spinal Cord: Characterization and Immunohistochemical Correlation

Magnetization Transfer Micro MR Imaging of Live Excised Lamprey Spinal Cord: Characterization and Immunohistochemical Correlation

... by immunohistochemical staining for glial cell protein showed distinct differences in their ana- tomic structure, with a larger LCM29-positive area percentage in the dorsal column and a lower percent- age ...

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Ectopic Prostate in the Uterine Cervix / Female Genital Tract Including Vagina and Vulva: A Review of the Literature

Ectopic Prostate in the Uterine Cervix / Female Genital Tract Including Vagina and Vulva: A Review of the Literature

... vagina. Immunohistochemical staining of the specimens revealed positive staining for prostate-specific antigen (PSA) in 13 out of 26 cases stained and positive staining for prostatic-specific ...

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Adenovirus mediated gene transfer into normal rabbit arteries results in prolonged vascular cell activation, inflammation, and neointimal hyperplasia

Adenovirus mediated gene transfer into normal rabbit arteries results in prolonged vascular cell activation, inflammation, and neointimal hyperplasia

... To test the hypothesis that adenovirus-mediated gene transfer results in inflammation and activation of the cells of the arterial wall, we performed immunohistochemical staining for ICAM[r] ...

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Melanin Bleaching with Warm Hydrogen Peroxide and Integrated Immunohistochemical Analysis: An Automated Platform

Melanin Bleaching with Warm Hydrogen Peroxide and Integrated Immunohistochemical Analysis: An Automated Platform

... H&E staining and IHC staining of heavily pigmented melanocytic lesions can be completed in a single ...automated immunohistochemical staining but with some additional heating and washing ...

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Original Article Application value of ultrasound contrast in gastric cancer examinations and its correlation with microvessel density

Original Article Application value of ultrasound contrast in gastric cancer examinations and its correlation with microvessel density

... analyzed. Immunohistochemical staining was adopted to calculate the microvessel density of cancerous tissues and adjacent normal tissues in patients receiving surgical treatment for gastric cancer, ...

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Metachronous rectal metastasis from primary transverse colon cancer: a case report

Metachronous rectal metastasis from primary transverse colon cancer: a case report

... Case presentation: The patient underwent a laparoscopic extended right hemicolectomy for primary transverse colon cancer. Histopathological examination showed moderately differentiated adenocarcinoma, and the tumor was ...

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Therapeutic Targeting of BRD4 in Head Neck Squamous Cell Carcinoma

Therapeutic Targeting of BRD4 in Head Neck Squamous Cell Carcinoma

... G-J. Immunohistochemical staining of BRD4 in samples from diverse stages in 4NQO-induced HNSCC animal ...BRD4 staining in normal (G), epithelial with hyperplasia (H), epithelial with severe ...

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