in vitro transfection
In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
... DC transfection were prepared using TATp derivatized with a lipid- terminated polymer capable of anchoring in the liposomal ...in vitro transfection efficiency in DC cultures as evidenced by a higher ...
11
Intracellular redox-responsive nanocarrier for plasmid delivery: in vitro characterization and in vivo studies in mice
... the transfection efficiency and cytotoxicity, its successful plasmid delivery in vitro and in vivo to realize its true therapeutic potentials remains a major challenge, mainly due to intracellular ...
12
Noga, Matthäus (2013): Bioresponsive HES-PEI conjugates for controlled shielding and deshielding of pDNA polyplexes. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... low transfection efficiency due to limited uptake and hindered endosomal ...in vitro by zeta potential measurements and, erythrocyte aggregation assay upon addition of α-amylase (AA) to the HES-decorated ...
153
Synergistic effect of reduced polypeptide micelle for co-delivery of doxorubicin and TRAIL against drug-resistance in breast cancer
... in vitro transfection efficiency, cytotoxicity, cellular apoptosis rate, in vivo distribution as well as anti- tumor effect of the LHRss/DOX/TRAIL construct were investigated in a drug-resistant breast ...
13
Screening for differentially expressed miRNAs in Aedes albopictus (Diptera: Culicidae) exposed to DENV 2 and their effect on replication of DENV 2 in C6/36 cells
... Mosquito genes influence the mosquito ’ s susceptibility to the virus, and the genotype of the mosquito may affect the choice of control strategies and may eventually provide clues to interrupt transmission [38]. In this ...
12
Acceleration of gene transfection efficiency in neuroblastoma cells through polyethyleneimine/poly(methyl methacrylate) core-shell magnetic nanoparticles
... gene transfection efficiency by accelerating the cellular uptake of ...gene transfection efficiency of the mag-PEI nanoparticles using plasmid pGL3-basic containing cytomegalovirus (CMV) promoter/enhancer ...
10
The sustained-release behavior and in vitro and in vivo transfection of <em>pEGFP</em>-loaded core-shell-structured chitosan-based composite particles
... in vitro for 15 days. Furthermore, in vitro transfection in human embryonic kidney 293T and human cervix epithelial cells, and in vivo transfection in mice skeletal muscle of loaded pEGFP, ...
14
Ruß, Verena (2008): Novel degradable pseudodendritic oligoamines for in vitro and in vivo gene delivery. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... and transfection efficiency demonstrated that pseudodendritic core characteristics as well as the surface modifications among the pseudodendritic core influence both transfection efficiency and cytotoxicity ...
118
Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
... in vitro cytotoxicity evaluation and transfection ...after transfection, the transfection ability of A-MN-SLN/DNA and B-MN-SLN/DNA was almost the same (P ...following transfection (P , ...
13
NOVEL LIPOSOMAL GENE DELIVERY REAGENTS: DESIGN, SYNTHESIS, IN VITRO AND IN VIVO TRANSFECTION PROPERTIES
... in vitro transfection efficiencies (in CHO, COS-1 and HepG2 cells) of eight novel amino-acid head-groups containing cholesterol based cationic lipids (lipids 1-8, Figure 8) are ...
14
Reciprocal regulation of PCGEM1 and miR-145promote proliferation of LNCaP prostate cancer cells
... by transfection of miR-145 or small interfering RNA sequences against (siRNA) ...miR-145 transfection on prostate cancer growth in vivo was examined in the (nu/nu) mouse ...expression. Transfection ...
10
Transfection in Agrobacterium tumefaciens
... Transfection frequencies in our study were optimal with a concentration of 22.5 Ag of phenol-extracted LR-4 phage DNA/ml.. Low DNA concentrations 1 and 10 ,ug/ml were not sufficient to p[r] ...
6
Complete nucleotide sequence of infectious Coxsackievirus B3 cDNA: two initial 5' uridine residues are regained during plus-strand RNA synthesis.
... The 5' termini of the transfection-derived viruses and that of parental CVB3 were found to be identical, demonstrating that recombinant plasmid DNA, as well as in vitro-synthesized RNA t[r] ...
11
In-Vitro Transcription and Transfection of HCV Genomic Replicons
... the transfection of Huh 7.5 derived cell lines with In-vitro transcript of HCV pF6/JFH-1 for production of infectious virus particles in naïve Huh ...2000 transfection reagent (Invitrogen, USA) ...in ...
7
Overexpression of miR-100 inhibits cell proliferation, migration, and chemosensitivity in human glioblastoma through FGFR3
... after transfection of the overexpression plasmids, U251 and T98g cells were transfected with lv-mir-100 for another 24 ...lentiviral transfection, a migration assay was performed in both U251 (E) and T98g ...
10
Stable Antigen Is Most Effective for Eliciting CD8+ T-Cell Responses after DNA Vaccination and Infection with Recombinant Vaccinia Virus In Vivo
... after transfection or infection with recombinant VV of cell lines in vitro was increased when introducing short-lived NP-fusion proteins, this was not observed for DNA vaccination and re- combinant VV ...
12
The Diversity of Torque Teno Viruses: In Vitro Replication Leads to the Formation of Additional Replication-Competent Subviral Molecules
... In vitro transcription. Detailed transcription patterns of TTV have been reported for the isolates TTV-P1C1 (35), TTV-HEL32 (18, 50), and TTV-HD3a (25). Three main mRNA species (1.0, 1.2 and 3.0 kb) had earlier ...
12
miR-142-3p reduces the size, migration and contractility of endometrial and endometriotic stromal cells by targeting integrin- and Rho GTPase- related pathways that regulate cytoskeletal function
... in vitro assays, this study identifies cytoskeletal regulation and integrin signaling as two relevant categories of miR-142-3p ...in vitro investigation, our study provides new mechanistic insights into the ...
15
Cytopathogenic and Noncytopathogenic RNA Replicons of Classical Swine Fever Virus
... low transfection efficiency, such as A187-⌬3390, the few cells which stain positive for NS3 ...in vitro transcription by T7 RNA polymerase or during initial low-level replication in ...
8
Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
... to transfection, the medium was removed and the cells were rinsed with phosphate-buffered saline (pH ...After transfection, the cells were washed twice with phosphate-buffered saline and grown in culture ...
9