Indirect immunofluorescence
The clinical significance of atypical indirect immunofluorescence patterns on primate cerebellum in paraneoplastic antibody screening
... atypical indirect immunofluorescence screening patterns of patients with a negative or absent typing assay for intraneuronal and anti-amphiphysin paraneoplastic ...reported immunofluorescence pattern ...
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Evaluation of an Indirect Immunofluorescence Assay for Strongyloidiasis as a Tool for Diagnosis and Follow-Up
... Strongyloidiasis is a chronic, soil-transmitted helminthiasis with a worldwide distribution. Current estimates indicate that it affects 30 to 100 million people, but accurate prevalence data are lacking. Although ...
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Comparison of a semiautomated fluorescent immunoassay system and indirect immunofluorescence for detection of antinuclear antibodies in human serum
... A semiautomated fluorescent immunoassay system FIAX for detecting antinuclear antibodies ANAs in human serum was compared with conventional indirect immunofluorescence using microscope s[r] ...
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Immunoglobulin G and immunoglobulin M polar staining of Toxoplasma gondii in the indirect immunofluorescence test
... Polar staining PS of Toxoplasma gondii in the indirect immunofluorescence IIF test has been described as a nonspecific reaction confined to the anterior pole of the protozoan and is pres[r] ...
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Detection of Epstein-Barr virus infection subtype in patients with multiple sclerosis by indirect immunofluorescence assay
... with indirect immunofluorescence for anti-EBV capsid antigen (EBV-CA) immunoglobulin G (IgG), IgG affinity for anti-EBV-CA, anti-EBV-CA immunoglobulin M (IgM), anti-EBV early antigen (EBV-EA) IgG and ...
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Indirect immunofluorescence test for serodiagnosis of Legionnaires disease: evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies
... Indirect Immunofluorescence Test for Serodiagnosis of Legionnaires Disease: Evidence for Serogroup Diversity of Legionnaires Disease Bacterial Antigens and for Multiple Specificity of Hu[r] ...
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Characterization of a panel of six β2-adrenergic receptor antibodies by indirect immunofluorescence microscopy
... To study β 2 AR trafficking in rat cells, either in vitro or in vivo , an antibody is needed that is capable of recognizing rat β 2 AR. Such an antibody might prove useful for localiz- ing native β 2 AR in rat lung ...
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Limited reliability of the indirect immunofluorescence technique for the detection of anti Rib P antibodies
... by indirect immunofluorescence, immunodiffusion, immunoblot, and other ...of indirect immunofluorescence using conventional HEp-2 substrates in the detection of sera with ribosomal P ...
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Automation in indirect immunofluorescence testing: a new step in the evolution of the autoimmunology laboratory
... Indirect immunofluorescence (IIF), a general method for detecting and measuring antibodies and autoantibodies, plays an important role within the immunological and immunometric assays (IMA) (reviewed in ...
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Application of an indirect immunofluorescence test for detection of Mycoplasma pneumoniae in respiratory exudates
... We prepared polyclonal antibody specific to Mycoplasma pneumoniae and examined the conditions influencing the ability of an indirect immunofluorescence test to detect the specific antige[r] ...
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Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae
... Members of the genus Rickettsia are Gram-negative strictly intracellular Alphaproteobacteria, multiplying by binary fission in the cytoplasm and nuclei of infected host cells [13, 19]. Rickettsiae are arthropod-vectored ...
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Subtyping Influenza A Virus with Monoclonal Antibodies and an Indirect Immunofluorescence Assay
... definitive immunofluorescence assay for the subtyping of influenza A virus isolates, we generated a panel of monoclonal antibodies (MAbs) against the major circulat- ing influenza A virus subtypes using multiple ...
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Simplified chromatographic separation of immunoglobulin M from G and its application to toxoplasma indirect immunofluorescence
... That such positive results in IIF for specific IgM 1, 11 results are caused by the blocking of IgM by IgG sera with antinuclear and antideoxyribonucleic in binding to the Toxoplasma anti[r] ...
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Typing of herpes simplex virus with type specific human immunoglobulin M in an indirect immunofluorescence assay
... Fixed cells infected with the McIntyre strain and cells infected with MS were overlaid with serial dilutions of the test serum, reference serum containing IgG reactive with both HSV-1 an[r] ...
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The Role of Indirect Immunofluorescence Methods in the Detection of Autoimmune Antibodies in Thyroid Disease
... We used an indirect immunofluoresence method (Euroimmun using Thyroid gland monkey with titer plane technique). Preparation of PBS Tween was carried out by dissolving 1 pack of PBS salt in 1 L of distilled water. ...
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Validation of Legionella pneumophila indirect immunofluorescence assay with epidemic sera
... The sensitivity of the IFA test as currently used by CDC was estimated to be at least that of the original test, on the basis of a comparison of titers against the Philadelphia 1 antigen[r] ...
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Interlaboratory and intralaboratory comparisons of indirect immunofluorescence assays for serodiagnosis of Lyme disease
... Therefore, serum specimens obtained from subjects at entry into the northern California epidemiologic study were subjected to a three-way interlaboratory comparison; this was done to det[r] ...
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Detection of microsporidia by indirect immunofluorescence antibody test using polyclonal and monoclonal antibodies
... During a screening for monoclonal antibodies MAbs to the microsporidian Encephalitozoon hellem, three murine hybridoma cell lines producing strong enzyme-linked immunosorbent assay ELISA[r] ...
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Comparison of indirect hemagglutination and indirect immunofluorescence tests with microneutralization tests for detection of type specific Herpesvirus hominis antibody
... Titer differences and 2/1 indexes in convalescent serum samples from patients with primary type 1 and type 2 infections tested by MNT, IHAT, and IFAT are shown in Table 2, and the sensit[r] ...
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Detection of antibody to murine cytomegalovirus by enzyme linked immunosorbent and indirect immunofluorescence assays
... At a serum dilution of 1:20, antibody to MCMV was not detected in sera of uninfected nul+ or nu/nu mice or in nul+ mice at 3 and 7 days after virus challenge.. Antibody to MCMV was detec[r] ...
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