insertional mutants
Identification of Zebrafish Insertional Mutants With Defects in Visual System Development and Function
... these mutants, the role for the disrupted set of genes that are involved in visual system development protein may not be specifically in retinal patterning, but and ...the mutants that displayed reduced MZs ...
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A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii
... Chlamydomonas insertional mutants became available to researchers ...new insertional mutants in their own ...the mutants have been vali- ...generating insertional mutants ...
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Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants
... isolate insertional mutants with specific phenotypes have been used successfully to identify genes involved in different metabolic and regu- latory pathways in the eukaryotic green alga Chlamydo- monas ...
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Arabidopsis Genes Essential for Seedling Viability: Isolation of Insertional Mutants and Molecular Cloning
... seedling-lethal mutants, which are likely to com- ...visible mutants in Arabidopsis. mutants have been isolated (Errampalli et ...the mutants identified in Castle et ...of mutants that ...
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Bidirectional-Genetics Platform, a Dual-Purpose Mutagenesis Strategy for Filamentous Fungi
... generating mutants to translate quickly gene sequences into their ...random insertional mutagenesis via transformation ...putative mutants, we isolated insertional mutants with ...
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Insertional Gene Activation by Lentiviral and Gammaretroviral Vectors
... eGFP insertional mutants, respectively, are also ...IL-3-independent mutants were selected either according to the IL-3 selection protocol (IL-3; RV1 to RV3) or in serum supplemented with 1 g/ml ...
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Insertional mutagenesis in transgenic mice generated by the pronuclear microinjection procedure
... nous labyrinth. At approximately one year of age, the organ of Corti is almost entirely missing throughout all the cochlear turns, and no inner or outer hair cells or supporting cells are present (Fig. 5A,B). The spiral ...
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Insertional mutagenesis of the Abelson murine leukemia virus genome: identification of mutants with altered kinase activity and defective transformation ability.
... Our analysis of these A-MuLV insertional mutants allows the division of the protein-coding region of the provirus into four domains: domain A proviral bp 1068 to 1685, in which insertion[r] ...
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Characterization of Essential Genes by Parasexual Genetics in the Human Fungal Pathogen Aspergillus fumigatus: Impact of Genomic Rearrangements Associated With Electroporation of DNA
... heterozygous insertional mutants were generated by electroporation of diploid conidia with a heterologous ...fumigatus insertional mutants has relatively limited value and cannot be used in an ...
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Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis.
... In mouse, the sequence rearrangements and deletions which accompany plas- mid insertion has complicated the isolation of genes in putative insertional mutants (MEISLER 19[r] ...
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Management of insertional Achilles tendinopathy through a Cincinnati incision
... Background: About 10% of patients not responding to 3–6 months of conservative management for insertional Achilles tendinopathy undergo surgery. Traditionally, surgery of the Achilles tendon is performed through ...
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Physiotherapy´s protocol to approach the insertional achilles tendinopathy
... This aim of this paper is to present a list of several Haglund disease cases that have been treated by conservative treatment, evaluating the results after 10 sessions by VISA-A scale t[r] ...
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Protein Determinants of Insertional Specificity for the Drosophila Gypsy Retrovirus
... It is possible that Su(Hw)-binding sequences targeting Integration of gypsy into ovo is determined by local gypsy to ovo could be present outside the 5⬘ovo region sequences contained within the gene: To understand used ...
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Insertional Achilles tendinopathy is associated with arthritic changes of the posterior calcaneal cartilage: a retrospective study
... Insertional Achilles tendinopathy (IAT) is a chronic de- generative process which often affects young active adults. Etiological factors include anatomical dysmor- physm of the posterior calcaneal tubercle, ...
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Insertional inactivation and cloning of the wA gene of Aspergillus nidulans.
... Transformation by integration in Podospora anserina. Re- placement of a chromosome segment by a two-step process. 1966 T h e induction and repression of nitrate reduc[r] ...
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Insertional protein engineering for analytical molecular sensing
... the insertional mutagenesis of the protease resist- ant, green fluorescent protein (GFP), to make it susceptible to trypsin and other proteolytic enzymes ...
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Transposon Mutagenesis of the Zika Virus Genome Highlights Regions Essential for RNA Replication and Restricted for Immune Evasion
... Insertions in the exposed surfaces of the envelope protein. Viral glycoproteins are a major target of neutralizing antibodies and are often under strong selective pressures. However, the capacity to tolerate sequence ...
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MEIOTIC CHROMOSOME BEHAVIOR OF AN INVERTED INSERTIONAL TRANSLOCATION IN NEUROSPORA
... When pairing fails between the translocated segment in 6 and its homologous region in chromo- some 1, buckles or loops are formed at pachynema in the deletion or insertion a[r] ...
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Development of high-titer retroviral producer cell lines by using Cre-mediated recombination.
... ian virus 40 promoter driving the TK gene; (v) a second loxP site; and (vi) a second Mo-MuLV LTR preceded by its poly- purine track. The configuration of this vector would allow selection of transduced cell lines with ...
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Functional antagonism of voltage gated K+ channel α subunits in the developing brain ventricular system
... indel mutants (indels in red) and their respective sequences and mutant with truncated kcnb1 polypeptide are ...In mutants #1, #2, a premature stop codon (TGA) was ...
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