Insulin-degrading enzyme (IDE)
Insulin-degrading enzyme is exported via an unconventional protein secretion pathway
... Insulin-degrading enzyme (IDE) is a ubiquitously expressed zinc-metalloprotease that degrades several pathophysiologically significant extracellular substrates, including insulin and ...
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Cellular distribution of insulin degrading enzyme gene expression Comparison with insulin and insulin like growth factor receptors
... Insulin-degrading enzyme (IDE) hydrolyzes both insulin and IGFs and has been proposed to play a role in signal termination after binding of these peptides to their ...of IDE mRNA ...
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Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells
... Background: Insulin-degrading enzyme (IDE) is a conserved zinc ...on IDE expression and activity in colorectal adenocarcinoma cell line ...Subsequently, IDE expression and ...
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Original Article The gene polymorphisms of insulin degrading enzyme (IDE) are associated with the risk of coronary heart disease in Chinese Han population
... Altered insulin degrading enzyme function leads to toxic amyloidand insulin aggregates, inflammation, and ...of insulin degrading enzyme (IDE) are associated with ...
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The Amino Terminus of Varicella-Zoster Virus (VZV) Glycoprotein E Is Required for Binding to Insulin-Degrading Enzyme, a VZV Receptor
... the IDE binding domain is likely important for its interaction with ...to IDE, we fused the first 71 amino acids of VZV gE to a portion of another herpesvirus glycoprotein to determine if the VZV gE ...
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Insulin-Degrading Enzyme Binds to the Nonglycosylated Precursor of Varicella-Zoster Virus gE Protein Found in the Endoplasmic Reticulum
... degradation enzyme (IDE) is a 110-kDa zinc metalloprotease found in the cytosol of all ...cells. IDE degrades insulin and a variety of small proteins including amyloid-  ...Recently, ...
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Involvement of insulin-degrading enzyme in the clearance of beta-amyloid at the blood-CSF barrier: Consequences of lead exposure
... of insulin, glucagon and Aβ ...by IDE substrates include hydrophobicity and the substrate's ability to aggregate readily to form fibrils ...[18,22,23]. IDE is known to be present in the cytosol, per- ...
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Statins induce insulin-degrading enzyme secretion from astrocytes via an autophagy-based unconventional secretory pathway
... Harvested cell pellets and media were prepared as de- scribed previously [47]. Briefly, cell pellets were lysed in RIPA buffer (50 mM Tris-Cl, pH 8.0, 150 mM NaCl, 1 % NP-40, 0.5 % NaDoc, 0.1 % SDS) containing protease ...
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Development of monoclonal antibodies and quantitative ELISAs targeting insulin-degrading enzyme
... of IDE remain unresolved, due in part to a lack of sufficiently selective reagents targeting this ubiquitous ...of IDE remains poorly defined. Although IDE is well-established to reside in cytosol ...
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Direct measurement of proinsulin in human plasma by the use of an insulin degrading enzyme
... this enzyme rapidly degrades insulin and insulin-like polypeptides to nonimmunoassayable components, whereas proinsulin and proinsulin cleaved at position B 54,55 are not appreciably ...of ...
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Bioconversion of Cellulosic Materials by the action of Microbial Cellulases
... Cellulases were initially investigated several decades back for the bioconversion of biomass which gave innovative way to research in industrial applications of the enzyme. It has become the third largest group ...
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Optimization of Aqueous Enzymatic Oil Extraction from Kernel of Oil Palm (Elaeis Guineensis) Using three Phase Partitioning and Microwave Irradiation
... and enzyme treatment should prove to be a very useful and powerful technique, particularly with its ease of scaling ...of enzyme, so as to reduce the cost of the entire ...
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Microbial catabolic pathways for complex aromatics
... The DNAN demethylase nucleotide sequences of JS1661 and their homologues in N. testacea are 27-54 % identical to each other, as shown in Table 4-3 and Fig 4-3. The amino acid sequences of the two peptides in JS1661 were ...
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Isolation of plastic degrading and L Asparaginase Enzyme Producing Bacteria from the Mangrove Environment of Guptapara, South Andaman
... A halophilic bacterium (Halococcus sp.) isolated from the mangrove sediments produced antitumor activity exhibiting L- Asparaginase enzyme (Savitri and Azmi, 2003). Lee et al. (2014) investigated the mangrove ...
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Screening of SDS-degrading bacteria from car wash wastewater and study of the alkylsulfatase enzyme activity.
... an enzyme that hydrolyses sulfate -ester bonds to give inorganic sulfate and ...alkylsulfatase enzyme activity and identify the alkylsulfatase enzyme coding ...
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The Phytophthora infestans haustorium is a site for secretion of diverse classes of infection associated proteins
... INF4 and PE1 are conventionally secreted from haustoria. To localize PE1-mRFP and INF4-mRFP during infection, they were transformed into P. infestans. The native signal peptides for secretion were retained. Free green ...
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Ralstonia solanacearum Type III Effector RipAY Is a Glutathione Degrading Enzyme That Is Activated by Plant Cytosolic Thioredoxins and Suppresses Plant Immunity
... GSH degradation assay. GSH (10 mM) was incubated with 3g of the RipAY or YER163c protein for 60 min at 30°C in 100 l of reaction mix- ture containing 50 mM Tris-HCl (pH 8.0). The reaction mixture was then incubated at ...
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Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing ability
... Results: In this study, the gene encoding ZHD101, a ZEN‑degrading enzyme produced by Clonostachys rosea IFO 7063, was cloned into an Escherichia coli–Lactobacillus shuttle vector, pNZ[r] ...
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Discovery and exploitation of the transcriptional regulatory system of pectinases in Aspergillus niger
... metabolism. The deletion of gaaX has the most profound effect on the transcript levels of the genes encoding the first three steps of the GA utilization pathway (gaaA, gaaB, and gaaC) and on the expression of gatA. ...
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Cognitive improvement of compound danshen in an Aβ25 35 peptide induced rat model of Alzheimer’s disease
... important enzyme that cleaved APP to produce ...Aβ degrading enzymes, IDE and NEP are thought to be most ...of IDE and NEP function could also lead to the elevation of Aβ level and therefore ...
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