Internal ribosome entry
Synergistic Internal Ribosome Entry Site/MicroRNA Based Approach for Flavivirus Attenuation and Live Vaccine Development
... ABSTRACT The recent emergence of Zika virus underscores the need for new strat- egies for a rapid development of safe flavivirus vaccines. Using another flavivirus (Langat virus [LGTV]) that belongs to the group of ...
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Functional involvement of polypyrimidine tract-binding protein in translation initiation complexes with the internal ribosome entry site of foot-and-mouth disease virus.
... A special mechanism for the initiation of translation signif- icantly different from the standard scheme of translation in eukaryotic cells plays a key role in the life cycle of picornavi- ruses (for recent reviews, see ...
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Neuroattenuation of Vesicular Stomatitis Virus through Picornaviral Internal Ribosome Entry Sites
... Vesicular stomatitis virus (VSV) is potent and a highly promising agent for the treatment of cancer. However, translation of VSV oncolytic virotherapy into the clinic is being hindered by its inherent neurotoxicity. It ...
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Viral Internal Ribosome Entry Site Structures Segregate into Two Distinct Morphologies
... An increasing number of viruses have been shown to initiate protein synthesis by a cap-independent mech- anism involving internal ribosome entry sites (IRESs). Predictions of the folding patterns of ...
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Interaction of Translation Initiation Factor eIF4B with the Poliovirus Internal Ribosome Entry Site
... Poliovirus translation is initiated at the internal ribosome entry site (IRES). Most likely involving the action of standard initiation factors, this highly structured cis element in the 5 ⴕ ...
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A Peptide Derived from RNA Recognition Motif 2 of Human La Protein Binds to Hepatitis C Virus Internal Ribosome Entry Site, Prevents Ribosomal Assembly, and Inhibits Internal Initiation of Translation
... (HCV) internal ribosome entry site (IRES) and stimulate ...of internal initiation of translation of HCV ...in ribosome assembly at the HCV IRES and also provides the basis for targeting ...
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The Picornavirus Avian Encephalomyelitis Virus Possesses a Hepatitis C Virus-Like Internal Ribosome Entry Site Element
... Avian encephalomyelitis virus (AEV) is a picornavirus that causes disease in poultry worldwide, and flocks must be vaccinated for protection. AEV is currently classified within the hepatovirus genus, since its proteins ...
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Conserved structural motifs located in distal loops of aphthovirus internal ribosome entry site domain 3 are required for internal initiation of translation.
... A comparison of picornavirus internal ribosome entry site (IRES) secondary structures revealed the exis- tence of conserved motifs located on loops. We have carried out a mutational analysis to test ...
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Functional Analyses of RNA Structures Shared between the Internal Ribosome Entry Sites of Hepatitis C Virus and the Picornavirus Porcine Teschovirus 1 Talfan
... Picornavirus genomes are infectious and function as mRNAs. Initiation of protein synthesis on picornavirus RNA is depen- dent on an internal ribosome entry site (IRES) (see reference 2 for a review). ...
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Tissue-Specific Replicating Capacity of a Chimeric Poliovirus That Carries the Internal Ribosome Entry Site of Hepatitis C Virus in a New Mouse Model Transgenic for the Human Poliovirus Receptor
... an internal ribosome entry site (IRES) has been discov- ered on many cellular mRNAs (37), as well as other viral RNAs, including hepatitis C virus (HCV) RNA (36, ...
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Translation Initiation at the CUU Codon Is Mediated by the Internal Ribosome Entry Site of an Insect Picorna-Like Virus In Vitro
... nine would also be the initiating amino acid when the capsid protein precursor of PSIV is translated by internal ribosome entry. The N-terminal amino acid of the capsid protein pre- cursor of PSIV is ...
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Identification of an essential region for internal initiation of translation in the aphthovirus internal ribosome entry site and implications for viral evolution.
... directs internal initiation of translation (32). This region, termed the internal ribosome entry site (IRES), is predicted to form extensive secondary structures and contains several unused ...
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A Dormant Internal Ribosome Entry Site Controls Translation of Feline Immunodeficiency Virus
... internal ribosome entry site (IRES) located within the 5 ⬘ UTR of the genomic RNA (2, 13, ...of internal ribosome entry is mediated by the IRES structure (28, 35, 42) with the ...
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Mutational Analysis of the GB Virus B Internal Ribosome Entry Site
... The most impressive difference between the two predicted structures is the inclusion of a large, approximately 97-nt in- sertion within domain II of the GBV-B 5⬘NTR. Computer modeling suggests the inserted sequence forms ...
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Replication of Poliovirus RNA with Complete Internal Ribosome Entry Site Deletions
... Poliovirus (PV), the prototypic member of the viral family Picornaviridae, is a positive-polarity RNA virus 7,441 nucleo- tides (nt) in length (44). PV RNA is composed of a 5⬘ non- translated region (NTR), an open ...
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The sequence element of the internal ribosome entry site and a 25-kilodalton cellular protein contribute to efficient internal initiation of translation of hepatitis C virus RNA.
... by internal entry of ribosomes into the 5 * noncoding region ...the internal ribosome entry site (IRES) and may involve host ...
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Identification of an Internal Ribosome Entry Segment in the 5′ Region of the Mouse VL30 Retrotransposon and Its Use in the Development of Retroviral Vectors
... To characterize the VL30m IRES, we used the canonical strategy of bicistronic constructs, first described by Pelletier and Sonenberg (70) and Jang et al. (44) (Fig. 2). The results, presented in Fig. 2B, show that the ...
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Studying Hepatitis C Virus: Making the Best of a Bad Virus
... As for all positive-strand RNA viruses, the HCV genome RNA is the centerpiece of replication, serving as a substrate for translation, replication, and packaging. These processes are controlled by “cis-acting” or “cis ...
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Viral Internal Ribosome Entry Site Elements: Novel Ribosome-RNA Complexes and Roles in Viral Pathogenesis
... The recently discovered HCV-like IRES elements in the CrPV-like viruses (30, 36) share many features of the HCV IRES. For example, the CrPV-like IRESs fold into a higher- ordered tertiary structure that contain three ...
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Mechanisms Governing the Selection of Translation Initiation Sites on Foot-and-Mouth Disease Virus RNA
... with ribosome scanning to the Lb site than any other mechanism, such as direct IRES-dependent internal ribosome entry at the Lb site ...scanning ribosome mechanism, the eIF1 requirement ...
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