internal transcribed spacer 1
PCR-Based Diagnosis of Neoscytalidium dimidiatum Infection Using Internal Transcribed Spacer 1 Region of Ribosomal DNA Primers
... Methods: N. dimidiatum-specific PCR primers were designed based on the sequence of the internal transcribed spacer 1 region, which is located between 18S and 5.8S nuclear rDNA. Fungal DNA ...
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Assessment of Ribosomal Large Subunit D1 D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
... A number of targets for the molecular identification of as- pergilli have been investigated including the mitochondrial cy- tochrome b gene (46, 57, 62), a putative aflatoxin pathway regulatory gene (aflR) (4), the DNA ...
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Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... Multiplex PCR amplification followed by either agarose gel electrophoresis (PCR-AGE) or microchip electrophoresis (PCR-ME) was used to test a total of 120 fungal strains. The internal transcribed ...
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Phylogenetic Classification and Species Identification of Dermatophyte Strains Based on DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1 Regions
... The mutual phylogenetic relationships of dermatophytes of the genera Trichophyton, Microsporum, and Epidermophyton were demonstrated by using internal transcribed spacer 1 (ITS1) region ...
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Phylogenetic Classification of Trichophyton mentagrophytes Complex Strains Based on DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1 Regions
... Using internal transcribed spacer 1 (ITS1) region ribosomal DNA sequences from 37 stock strains and clin- ical isolates provisionally termed Trichophyton mentagrophytes complex in Japan, we ...
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Computational Approach Involving Use of the Internal Transcribed Spacer 1 Region for Identification of Mycobacterium Species
... The rapid and reliable identification of clinically significant Mycobacterium species is a challenge for diagnostic laboratories. This study evaluates a unique sequence-dependent identification algorithm called MycoAlign ...
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DNA Microarray Based on Arrayed Primer Extension Technique for Identification of Pathogenic Fungi Responsible for Invasive and Superficial Mycoses
... the internal transcribed spacer 1 and 2 (ITS1 and ITS2) region were designed for 24 species belonging to 10 genera, including Candida species (Candida albicans, Candida dubliniensis, Candida ...
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Phenotypic and Molecular Characterization of Madurella pseudomycetomatis sp nov , a Novel Opportunistic Fungus Possibly Causing Black Grain Mycetoma
... of internal transcribed spacer 1 (ITS1)-ITS2 region, it has maximum se- quence identity (93%) with Madurella mycetomatis, one of the main microorganisms causing black-grain fungal ...
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Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens
... We describe the development and evaluation of a novel, rapid MT-PCR assay for the detection and identification of fungi directly from positive blood cultures. The assay identifies seven Candida species (C. albicans, C. ...
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CUTANEOUS LEISHMANIASIS IN SYRIA
... the internal transcribed spacer 1 (ITS1) for identification of leishmania at species level, on 37 samples were obtained from patients with suspected CL referred to Damascus Dermatology ...
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Phylogenetic Analysis of The Internal Transcribed Spacer (ITS) Region in Laccase Producing Pseudomonas aeruginosa ADN04 by Predicted rRNA Secondary Structure
... the internal transcribed spacer- 1 (ITS1) in laccase producing organism were studied among 35 species through molecular phylogenetic ...
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Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2
... ITS 1 and 2 sequence analysis can be used for recognition of many fungal genera, including those that do not typically cause invasive disease such as airborne allergenic ...
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PCR Analyses of tRNA Intergenic Spacer, 16S 23S Internal Transcribed Spacer, and Randomly Amplified Polymorphic DNA Reveal Inter and Intraspecific Relationships ofEnterobacter cloacae Strains
... The most striking data presented in this study were that the use of tDNA-PCR and ITS-PCR together offered a hierarchi- cal system for E. cloacae strain differentiation; tDNA-PCR resolved the cultures at the species level ...
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Differentiation between Mycobacterium farcinogenes and Mycobacterium senegalense Strains Based on 16S 23S Ribosomal DNA Internal Transcribed Spacer Sequences
... Bacterial lysates were obtained and used for PCR amplifi- cation of three different ribosomal regions as described else- where (24, 25). i.e., (i) the 5⬘ region of 16S rDNA (24), (ii) the complete ITS (7, 24), and (iii) ...
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Typing of Histoplasma capsulatumIsolates Based on Nucleotide Sequence Variation in the Internal Transcribed Spacer Regions of rRNA Genes
... cetous teleomorph form of H. capsulatum, deposited in the GenBank (accession number U18363). The sequence 5⬘-CGGAAGGATCATTACCACGCCG-3⬘, lo- cated at nucleotide positions 39 to 60, was chosen as the 5⬘ primer (designated ...
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Internal Transcribed Spacer Regions of rRNA Genes of Pneumocystis carinii from Monkeys
... Pneumocystis carinii is the major cause of pneumonia in AIDS patients and is one of the most common opportunistic pathogens in immunocompromised patients. P. carinii is also found in many different species of vertebrate ...
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Identification of Medically Important Yeast Species by Sequence Analysis of the Internal Transcribed Spacer Regions
... The GenBank database currently lacks ITS sequence entries for some yeast species. However, the number of ITS sequences available in public databases has increased rapidly in recent years, and the expanding database may ...
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Identification of Clinical Staphylococcal Isolates from Humans by Internal Transcribed Spacer PCR
... lugdunensis, and 1 each of S. caprae, S. carnosus, and S. cohnii. The remaining 25 isolates not identified by ITS-PCR were all gram-positive cocci that produced catalase and gave negative results in the modified ...
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Patterns of Variation in the Intergenic Spacers of Ribosomal DNA in Drosophila melanogaster Support a Model for Genetic Exchanges During X-Y Pairing
... external transcribed spacer After 3 days, the same male was removed and crossed to ...indicate spacer regions: IGS, intergenic C(1)DX, y f lz virgin females (stock 06600 from Umea˚ Drosoph- ...
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Morphological and Molecular Characterization of Exophiala polymorpha sp nov Isolated from Sporotrichoid Lymphocutaneous Lesions in a Patient with Myasthenia Gravis
... E xophiala species are phaeoid, conidial fungi that are widely distributed in decaying wood, soil, plants, and water. They are capable of causing phaeohyphomycosis, including localized infec- tions following traumatic ...
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