Internal Transcribed Spacer Region
Molecular Systematics of Dictyostelids: 5.8S Ribosomal DNA and Internal Transcribed Spacer Region Analyses
... ITS1 regions are 278 to 312 nt long in Dictyostelium spp. and Acytostelium spp. and 192 to 253 nt long in Polysphondylium spp., with the exception of that of Acytostelium leptosomum, which is 245 nt long (data not ...
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Polymorphic Internal Transcribed Spacer Region 1 DNA Sequences Identify Medically Important Yeasts
... Identification of unusual isolates. In a previous study (2), we observed ⬎98% concordance between biochemical and ITS2 genotypic identifications validated with over 400 clinical iso- lates. However, four clinical ...
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Analysis of the 16S–23S rRNA Gene Internal Transcribed Spacer Region in Klebsiella Species
... nal transcribed spacer (ITS) sequence, which is not subject to the same selective pressure as the rRNA genes and conse- quently has a 10-times-greater evolution rate, appears to be able to overcome the ...
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Chronic Kirschsteiniothelia infection superimposed on a pre existing non infectious bursitis of the ankle: the first case report of human infection
... Case presentation: An 81-year-old immunocompetent female local farmer noticed the presence of a nodule on her right ankle 5 years before her first visit to our hospital. A cystic mass of approximately 45 mm × 30 mm was ...
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Mitochondrial introgression and interspecies recombination in the Fusarium fujikuroi species complex
... The Fusarium fujikuroi species complex (FFSC, previously referred to as the Gibberella fujikuroi species complex) is one of several monophyletic assemblages in the genus Fusarium (phylum Ascomycota, order Hypocreales) ...
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rRNA Gene Internal Transcribed Spacer 1 and 2 Sequences of Asexual, Anthropophilic Dermatophytes Related toTrichophyton rubrum
... variable region of fungal DNA, the ribosomal region consisting of internal transcribed spacer sequences (ITS) 1 and 2 and their intermediary ...ITS1 region of certain der- ...
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Computational Approach Involving Use of the Internal Transcribed Spacer 1 Region for Identification of Mycobacterium Species
... the internal transcribed spacer 1 (ITS-1) region of the rRNA gene complex from characterized Mycobacterium ...ITS-1 region sequence over the 16S rRNA gene sequence as a target for ...
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Identification and First Report of Inonotus (Phellinus) tropicalis as an Etiologic Agent in a Patient with Chronic Granulomatous Disease
... and internal transcribed spacer (ITS) region sequence data (described below) indicated that the fungus was a wood-decaying basidiomycete, three iso- lates, ...
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Genetic comparison of different human population groups using internal transcribed spacer sequence
... ITS region has not yet being used in genetic identity test in ...variable region make this an attractive option compared to existing identification markers (Blouin, ...
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Discrimination of Aspergillosis, Mucormycosis, Fusariosis, and Scedosporiosis in Formalin Fixed Paraffin Embedded Tissue Specimens by Use of Multiple Real Time Quantitative PCR Assays
... the internal transcribed spacer 2 (ITS2) region of ribosomal DNA, using fluorescently labeled primers, was performed to identify clinically important genera and species of Aspergillus, ...
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Candida inconspicua and Candida norvegensis: New Insights into Identification in Relation to Sexual Reproduction and Genome Organization
... the internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA), the D1/D2 domain of the 28S rDNA, and the elongation factor 1 ␣ (EF-1 ␣ ) gene, either directly or following cloning, ...
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Development and Clinical Application of a Panfungal PCR Assay To Detect and Identify Fungal DNA in Tissue Specimens
... the internal transcribed spacer 1 (ITS1) region of the ribosomal DNA gene cluster to detect fungal DNA in fresh and formalin-fixed, paraffin-embedded (PE) tissue specimens from patients with ...
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High Throughput Detection of Pathogenic Yeasts of the Genus Trichosporon
... The need for a rapid and accurate method for the detection of fungal pathogens has become imperative as the incidence of fungal infections has increased dramatically. Herein, we tested the Luminex 100, a novel flow ...
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PCR-Based Diagnosis of Neoscytalidium dimidiatum Infection Using Internal Transcribed Spacer 1 Region of Ribosomal DNA Primers
... The primer specificity and sensitivity (lower limit of detection) of N. dimidiatum-specific PCR for standard DMP and NDM strains were evaluated using specific primers (Table 1). Two strains of N. dimidiatum demonstrated ...
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Re-emergence of Lilium callosum Sieb. et Zucc. in Taiwan after a fire allows propagation and renews the possibility of conservation
... The application of molecular techniques, such as DNA sequencing, for phylogenetic analysis has successfully distinguished morphological similarity from homo- plasy (Nishikawa et al. 1999). The phylogeny of numer- ous ...
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... the Internal Transcribed Spacer (ITS) region and optimization of PCR-RFLP using a dermatophyte specific primer targeted the 18S ribosomal DNA (rDNA) region were performed for the ...
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Phenotypic and Molecular Characterization of Madurella pseudomycetomatis sp nov , a Novel Opportunistic Fungus Possibly Causing Black Grain Mycetoma
... the internal transcribed spacer (ITS) region and the D1/D2 hypervariable region of the 28S ribosomal gene sequences support a new species ...
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DNA Microarray Based on Arrayed Primer Extension Technique for Identification of Pathogenic Fungi Responsible for Invasive and Superficial Mycoses
... the internal transcribed spacer 1 and 2 (ITS1 and ITS2) region were designed for 24 species belonging to 10 genera, including Candida species (Candida albicans, Candida dubliniensis, Candida ...
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Phylogenetic Analysis of The Internal Transcribed Spacer (ITS) Region in Laccase Producing Pseudomonas aeruginosa ADN04 by Predicted rRNA Secondary Structure
... Secondary structure and phylogenetic efficacy of the internal transcribed spacer- 1 (ITS1) in laccase producing organism were studied among 35 species through molecular phylogenetic analyses. The ...
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Identification to the Species Level and Differentiation between Strains of Aspergillus Clinical Isolates by Automated Repetitive Sequence Based PCR
... A commercially available repetitive-sequence-based PCR (rep-PCR) DNA fingerprinting assay adapted to an automated format, the DiversiLab system, enables rapid microbial identification and strain typing. We ex- plored the ...
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