Internal transcribed spacer region 1
Polymorphic Internal Transcribed Spacer Region 1 DNA Sequences Identify Medically Important Yeasts
... (Table 1): for 18 species, multiple isolates were characterized, and for 22 species, only single isolates were characterized for PCR product ...(Table 1) were subsequently confirmed by direct sequence ...
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Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2
... contiguous internal transcribed spacer (ITS) region, ITS ...ITS 1 and ITS 2 regions were needed for accurate identification of Aspergillus at the species ...ITS 1 and 2 ...
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PCR-Based Diagnosis of Neoscytalidium dimidiatum Infection Using Internal Transcribed Spacer 1 Region of Ribosomal DNA Primers
... The primer sequence of N. dimidiatum was designed using MEGA4 alignment software (http:// www.megasoftware.net/download_form) and Primer Express software (Applied Biosystems, Foster City, CA, USA) based on upstream and ...
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Phylogenetic Analysis of The Internal Transcribed Spacer (ITS) Region in Laccase Producing Pseudomonas aeruginosa ADN04 by Predicted rRNA Secondary Structure
... the internal transcribed spacer- 1 (ITS1) in laccase producing organism were studied among 35 species through molecular phylogenetic ...
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rRNA Gene Internal Transcribed Spacer 1 and 2 Sequences of Asexual, Anthropophilic Dermatophytes Related toTrichophyton rubrum
... The segment of rDNA sequenced in this investigation in- cluded 164 bases at the 3⬘ end of the small-subunit RNA gene, the entire ITS1 and ITS2 regions and the 5.8S gene, and 81 bases at the 5⬘ end of the large-subunit ...
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Re-emergence of Lilium callosum Sieb. et Zucc. in Taiwan after a fire allows propagation and renews the possibility of conservation
... The application of molecular techniques, such as DNA sequencing, for phylogenetic analysis has successfully distinguished morphological similarity from homo- plasy (Nishikawa et al. 1999). The phylogeny of numer- ous ...
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Development and Clinical Application of a Panfungal PCR Assay To Detect and Identify Fungal DNA in Tissue Specimens
... the internal transcribed spacer 1 (ITS1) region of the ribosomal DNA gene cluster to detect fungal DNA in fresh and formalin-fixed, paraffin-embedded (PE) tissue specimens from patients ...
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Assessment of Ribosomal Large Subunit D1 D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
... D1-D2 region, the two ITS regions investigated were found to exhibit localized hypervariable regions contain- ing most of the intraspecies sequence ...both spacer re- gions; exceptions included sequevars of ...
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DNA Microarray Based on Arrayed Primer Extension Technique for Identification of Pathogenic Fungi Responsible for Invasive and Superficial Mycoses
... the internal transcribed spacer 1 and 2 (ITS1 and ITS2) region were designed for 24 species belonging to 10 genera, including Candida species (Candida albicans, Candida dubliniensis, ...
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Identification and First Report of Inonotus (Phellinus) tropicalis as an Etiologic Agent in a Patient with Chronic Granulomatous Disease
... and internal transcribed spacer (ITS) region sequence data (described below) indicated that the fungus was a wood-decaying basidiomycete, three iso- lates, ...at 1 week and 83 to 85 mm ...
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Phenotypic and Molecular Characterization of Madurella pseudomycetomatis sp nov , a Novel Opportunistic Fungus Possibly Causing Black Grain Mycetoma
... of internal transcribed spacer 1 (ITS1)-ITS2 region, it has maximum se- quence identity (93%) with Madurella mycetomatis, one of the main microorganisms causing black-grain fungal ...
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Analysis of the 16S–23S rRNA Gene Internal Transcribed Spacer Region in Klebsiella Species
... Amplification of the ITS. To design the primers for the PCR amplification of Klebsiella ITS regions, all the available 16S and 23S rRNA gene sequences of the genus in the the Ribosomal Data Project ...
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Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... The internal transcribed spacer 1 (ITS1) and ITS2 regions and the ...(rDNA) region of the fungi were amplified by using universal primers ITS1 and ...ITS2 region was ...
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Phylogenetic Classification and Species Identification of Dermatophyte Strains Based on DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1 Regions
... The mutual phylogenetic relationships of dermatophytes of the genera Trichophyton, Microsporum, and Epidermophyton were demonstrated by using internal transcribed spacer 1 (ITS1) region ...
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Molecular phylogeny of selected mango cultivars based on internal transcribed spacer (ITS) region
... Ramirez, I. M., Rodriguez, N. N., Valdes-Infante, J., Capote, M., Becker, D., Rohde, W. (2004). Isolation of Genomic DNAs from the Tropical Fruit Trees Avocado, Coconut, Guava and Mango for PCR-based DNA Marker ...
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Molecular Systematics of Dictyostelids: 5.8S Ribosomal DNA and Internal Transcribed Spacer Region Analyses
... The sizes of the 5.8S rDNA varied between 161 and 166 nucleotides (nt) (data not shown). Multiple alignment of the sequences, made using the ClustalW program (12), allowed us to determine the percentage of nucleotide ...
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Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens
... fungal internal transcribed spacer 1 (ITS1) and ITS2 region, elongation factor 1- ␣ (EF1- ␣ ), and  -tubulin genes to identify 11 fungal pathogens: Candida albicans, Candida ...
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Computational Approach Involving Use of the Internal Transcribed Spacer 1 Region for Identification of Mycobacterium Species
... Custom database design and validation. The MycoAlign system was imple- mented in a PostGreSOL relational database using JAVA programming lan- guage on a Linux platform. The sequence analysis program used an optimal ...
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Rapid Differentiation of Aspergillus Species from Other Medically Important Opportunistic Molds and Yeasts by PCR Enzyme Immunoassay
... cultures and a commercial DNA extraction kit (PureGene). A commercially available cell disruption and DNA extraction kit was also used to process DNA from infected rabbit tissues (Qbiogene). This latter system has been ...
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Mitochondrial introgression and interspecies recombination in the Fusarium fujikuroi species complex
... the internal transcribed spacer region of the ribosomal RNA genes, suggests that the origin and history of this complex likely involved interspecies gene % X species should harbour ...
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