Invasion assays
Long non-coding RNA H19 regulates FOXM1 expression by competitively binding endogenous miR-342-3p in gallbladder cancer
... reporter assays, RNA-binding protein immunoprecipitation (RIP) and RNA pull-down assays, we verified that H19 was identified as a direct target of ...Western-blotting assays demonstrated that H19 ...
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<p>LINC00491 as a new molecular marker can promote the proliferation, migration and invasion of colon adenocarcinoma cells</p>
... formation assays, si-LINC00491-123# decreased cell proliferation (Figure 2C and ...and invasion of colon adenocarcinoma ...transwell invasion assays were performed in HT-29 and HCT116 cells ...
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Original Article FAM129A promotes invasion and proliferation by activating FAK signaling pathway in non-small cell lung cancer
... Cell invasion assays were performed with 20 μl matrigel (1:3 dilution; BD Bioscience, San Jose, CA, USA) coated on the upper surface of 24-well Transwell chambers with 8-μm pores (Costar, Cambridge, MA, ...
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lncRNA FBXL19-AS1 regulates osteosarcoma cell proliferation, migration and invasion by sponging miR-346
... Cellular invasion was measured using transwell chambers (8 µ m pores; Corning Inc., Corning, NY, USA). Cells growing in the log phase were trypsinized, resuspended in serum-free medium, and seeded into chambers. ...
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Cross-linked hyaluronic acid gel inhibits metastasis and growth of gastric and hepatic cancer cells: in vitro and in vivo studies
... and invasion assays, Western blotting, pull down assay, siRNA interference, and nude mice implantation tumor model were applied in this ...and invasion activities, the MAPK and PI3K/Akt mediated ...
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A novel long non-coding RNA lnc-GNAT1-1 is low expressed in colorectal cancer and acts as a tumor suppressor through regulating RKIP-NF-κB-Snail circuit
... Then, to elucidate the role of lnc-GNAT1-1 in tumor pro- gression, we down-regulated the lnc-GNAT1-1 expression using siRNA or a negative control in SW480 cells. The inhibitory rate (lnc-GNAT1-1 siRNA vs. negative ...
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MARCH5 overexpression contributes to tumor growth and metastasis and associates with poor survival in breast cancer
... or empty vector into MCF-7 and T47D cells, which have relative low MARCH5 expression (shown in Figure 1A, B). Upregulation of MARCH5 was evidenced by qRT-PCR and Western blot analysis (Figure S2A, B). As shown in Figure ...
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Original Article miR-761 inhibits human osteosarcoma progression by targeting CXCR1
... and invasion assays, we revealed that overexpres- sion of miR-761 inhibits cell proliferation and invasion in vitro and validated CXCR1 was a functional target of miR-761 to participate the cell ...
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Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling
... and invasion assays were ...MTT assays showed no significant effects of GALNT1 on HCC cell viability (data not ...matrigel invasion assays demonstrate that overexpression of GALNT1 ...
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Original Article miR-506, downregulated in glioma, inhibits cell growth and disrupts the cell cycle by regulating YAP
... proliferation assays, invasion assays, migration assays, apoptosis assays and cycle assays were used after co-transfection of miR-506 mimics and Lipofectamine TM ...reporter ...
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Probiotic Bacteria and their Supernatants Protect Enterocyte Cell Lines from Enteroinvasive Escherichia coli (EIEC) Invasion
... and invasion to intestinal ...with invasion of enteroinvasive Escherichia coli (EIEC) strain, using human colonic adenocarcinoma cell lines, T84 and Caco2 ...assess invasion of Caco-2 and T84 cells ...
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miR-29a promotes osteoblast proliferation by downregulating DKK-1 expression and activating Wnt/β-catenin signaling pathway
... and invasion were determined us- ing a Transwell system (Corning, ...The invasion assay was performed using Matrigel ma- trix (BD Biosciences, San Jose, USA) as a barrier through which the cells had to ...
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<p>miR-26b-5p Inhibits the Proliferation, Migration and Invasion of Human Papillary Thyroid Cancer in a β-Catenin-Dependent Manner</p>
... A transwell assay was performed to measure the migration and invasion ability. A total of 4×10 4 cells/well were resuspended in 250 μ L of plain medium without any FBS in the upper chamber (8- μ m pore size, ...
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Original Article MiR-130a regulates the proliferation and metastasis of HCC cells through targeting ZEB1/2
... tachment assays showed that forced expres- sion of miR-130a significantly inhibited HCC cells metastasis, while deletion of miR-130a obviously facilitated cells metastasis (Figure ...
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<p>Circular RNA circHIPK3 promotes cell proliferation and invasion of prostate cancer by sponging miR-193a-3p and regulating MCL1 expression</p>
... RiP assays were used to detect circhiPK3 binding with agO2 complex in PC3 and DU145 ...capture assays were used to detect which miRna binds with circhiPK3 in PC3 ...
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<em>Axl </em>promotes the proliferation, invasion and migration of Wilms’ tumor and can be used as a prognostic factor
... testing the signal proteins in Akt pathway, the expressions of PI3K, Akt, 4EBP1 and P70S6K were dramatically reduced after Axl knockdown. Consequently, the effect of Axl on proliferation, invasion and migration of ...
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<p>LINC01272 Promotes Migration and Invasion of Gastric Cancer Cells via EMT</p>
... and invasion abilities were assessed by wound-healing and Transwell ...Transwell invasion assay, invasion was assessed based on the migration experi- ...
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LINC01503/miR-342-3p facilitates malignancy in non-small-cell lung cancer cells via regulating LASP1
... promote the malignant phenotype of NSCLC via indu- cing phosphorylation of the FAK-AKT pathway [28]. From our experiments, LASP1 had a higher expression levels in tumor samples, which was consistent with pre- vious ...
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Long noncoding RNA CASC2 suppresses esophageal squamous cell carcinoma progression by increasing SOCS1 expression
... LncRNAs exert their functions through diverse mecha- nisms, including transcriptional regulation, scaffolding of nuclear or cytoplasmic complexes, post-transcriptional regulation and post-translational modification [22, ...
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Detection of hepatocyte growth factor/scatter factor receptor (c Met) in axillary drainage after operations for breast cancer using reverse transcriptase–polymerase chain reaction
... The resected specimen was covered circumferentially by black Indian ink and Bouins solution and then was sliced into 5 mm slices. Each slice was evaluated macroscopi- cally for the presence of tumor and its distance from ...
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