isoelectric point
IPC – Isoelectric Point Calculator
... of Isoelectric Point Calcu- lator 15, other pKa sets and two programs based on SVM (pIR) and ANN (pIPredict) were ...tested. Isoelectric point predictions were validated separately for ...
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Using isoelectric point to determine the pH for initial protein crystallisation trials
... The isoelectric point determines a protein’s minimum solubility level due to protein–protein interactions being favoured over pro- tein–water interactions (Gilliland, 1988; Luft et ...the isoelectric ...
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Determination of surface ? potential and isoelectric point of carbon surfaces using tracer particle suspensions
... and isoelectric point of macroscopic carbon surfaces was ...establishing isoelectric points, an important requirement for understanding the behaviour of macroscopic carbon surfaces in aqueous ...
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Shift in the isoelectric-point of milk proteins as a consequence of adaptive divergence between the milks of mammalian species
... in isoelectric point during ...the isoelectric point shift, since residues that show significant evidence of positive selection are much more likely to be charged (p = ...
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An Evolutionary Computation Approach to Optimization of Isoelectric Point Prediction in Proteins
... The top 5% fittest mated value parameters being to the pi prediction algorithm for example, the function looks arginine one spot and sees the it the amino acid corresponding pKa the acid[r] ...
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Flow Behaviour of Gliadin in Absence and Presence of Ionic Surfactants
... minimum point, which corresponds to the isoelectric pH ...of isoelectric point, the viscosity depends on factors such as hydrodynamic volume, net charge on the molecule, existence of free ions ...
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Protein profiling of the dimorphic, pathogenic fungus, Penicillium marneffei
... pI/ M r (kDa), experimental isoelectric point and molecular mass estimated from the 2DGE gels; Species, the fungal species of the matched protein; Mascot score, score obtained from the [r] ...
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ERK and AKT phosphorylation status in lung cancer and emphysema using nanocapillary isoelectric focusing
... to isoelectric point ( pI); the pI is the pH at which individual protein residues remain stationary in the pH gradient when applying an electrical ...
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Genetic heterogeneity in partial adenosine deaminase deficiency
... immunologically normal children from four different "families." These children lack ADA in their erythrocytes but retain variable amounts of activity in their lymphoid cells. We have examined ADA activity in ...
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Zeta Potentials and Isoelectric Points of Biomolecules: The Effects of Ion Types and Ionic Strengths
... The isoelectric point (IEP) is the pH of a protein solution at which the net charge or zeta potential of protein is ...the isoelectric point due to the measurement ...the isoelectric ...
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Electrostatic complexation of polyelectrolyte and magnetic nanoparticles: from wild clustering to controllable magnetic wires
... At isoelectric point, we obtained large aggregates of 100 μm with irregular morphologies, indicating the strong attractive interaction between homoPEs with charged NPs and their uncontrolled ...
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Sensitivity and Specificity Determinations with Isoelectric Focusing Fractions of Blastomyces dermatitidis for Antibody Detection in Serum Specimens from Infected Dogs
... (the isoelectric point). When proteins reach their unique isoelectric point in this pH gradient they are no longer able to migrate and forced to remain where their net charge is zero by the ...
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The kl-3 loop of the Y chromosome of Drosophila melanogaster binds a tektin-like protein.
... These experiments showed that this antibody recognizes a single protein spot having an isoelectric point around 8.3, well sep- arated from the tubulins which migrate to a lower p[r] ...
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A study of the effects of salt and of pH on precipitation of Antigen-antibody compounds
... bility of HSA-antibody complex in salt-free solutions; the complex can precipitate in the absence of salt when the pH is near the isoelectric point of antibody, probably because. antibod[r] ...
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Characterization of urease from Campylobacter pylori
... The enzyme appears to be unique with respect to size, electrophoretic mobility, isoelectric point, and sensitivity to urease inhibitors when compared with the ureases of Proteus, Provide[r] ...
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Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides
... By designing 15 different model SLPs with a wide range of physicochemical properties, in particular with respect to isoelectric point (pI) and hydrophobicity, all harboring the SIINFEKL [r] ...
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Review on the extraction of biomolecules by biosurfactant reverse micelles
... 2) The water phase pH of the solution determines the net charge of biomolecules. The pH must be at a level that generates a biomolecule net charge opposite in sign to the surfactant headgroup, so that there is an ...
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Isoelectric Focusing pdf
... This equation shows that good resolution should be obtained with substances with a low diffusion coef R c- ient (D) and a high mobility slope [d /d(pH)] at the isoelectric point } conditions that are satis ...
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Enzymatic modifications of human fibroblast and leukocyte interferons.
... Treatment with neuraminidase and ,Bgalactosidase changed the isoelectric point distribution but did not affect the activity or thermal stability of either interferon; such a treatment re[r] ...
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Phenotypes of apolipoprotein B and apolipoprotein E after liver transplantation
... phenotypes before and after liver transplantation to gain new insights into apolipoprotein physiology. In all 29 patients that we studied, the postoperative serum apoE phenotype of the recipient, as assessed by ...
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