isolated nuclei
3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei
... from isolated nuclei labeled with repetitive sequences or BACs specific for chromo- somes 2 and 4 are presented in Figure ...retain nuclei within the context of their intact tissue environment and ...
7
State of Adenovirus 2 Deoxyribonucleic Acid in the Nucleus and Its Mode of Transcription: Studies with Isolated Viral Deoxyribonucleic Acid-Protein Complexes and Isolated Nuclei
... State of Adenovirus 2 Deoxyribonucleic Acid in the Nucleus and Its Mode of Transcription: Studies with Isolated Viral Deoxyribonucleic AcidProtein Complexes and Isolated Nuclei Received [r] ...
9
Replication of polyoma DNA in isolated nuclei: analysis of replication fork movement.
... Incubations were done at Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The movement of replication forks during polyoma DNA synthesis in isolated nuclei was analyzed [r] ...
8
Synthesis and Processing of Adenoviral RNA in Isolated Nuclei
... Hybridization of RNA to XhoI fragments of adenovirus type 2 DNA transferred to nitrocellulose filters reveals that sequences complementary to the region from 22.0 to 26.5 units present i[r] ...
10
Thyroid hormone action Demonstration of similar receptors in isolated nuclei of rat liver and cultured GH1 cells
... culture, isolated GH1 cell nuclei, or rat liver ...in isolated GH1 cell nuclei and rat liver nuclei, and both values were similar to that determined in intact GH2 ...
5
Transcription of the bovine parvovirus genome in isolated nuclei.
... We have found essentially no changes in the endogenous RNA polymerase activities and ammonium sulfate optima in nuclei isolated from both BPVinfected and mock-infected cells, suggesting [r] ...
6
Autoregulation of Adenovirus Type 5 Early Gene Expression III. Transcription Studies in Isolated Nuclei
... At a restrictive temperature, early virus RNA accumulation in the nuclei and cytoplasm of infected cells is two- to sevenfold higher in ts125 infections than in wild-type WT infections a[r] ...
8
Distribution of Replicating Simian Virus 40 DNA in Intact Cells and Its Maturation in Isolated Nuclei
... However, the fundamental problem of separating sibling chromosomes as two replication forks Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The maturation of replicatin[r] ...
16
Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.
... Because little self-complementary RNA was Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Nuclei isolated from herpes simplex virus HSV-infected KB cells actively synth[r] ...
5
Replication of polyoma DNA in isolated nuclei. V. Complementation of in vitro DNA replication.
... Prelabeled, depleted nuclei were incubated at two concentrations of nuclei with increasing amounts of cytoplasmic protein extracts from polyoma-infected cells microliters.. Incubations w[r] ...
9
Isolated Nucleus Stiffens in Response to Low Intensity Vibration
... Poly-L-Lysine. Isolated nuclei are incubated for 25 minutes in 37˚C with 1mL 1X PBS for adhesion to substrate then subjected to AFM testing for up to 1 ...of isolated nuclei in 2% ...
78
Structural topography of simian virus 40 DNA replication.
... Isolated nuclei as well as nuclear matrices were able to continue SV40 DNA elongation under replication conditions in situ, arguing for a coordinated and functional association of SV40 D[r] ...
11
Specific nuclear binding of adenosine 3',5' monophosphate binding protein complex with subsequent poly(A) RNA synthesis in embryonic chick cartilage
... complexes (41,000, 51,000, and 55,000 daltons). The 51,000 and 55,000 dalton cyclic AMP binding proteins were further purified by DNA-cellulose chromatography. In the presence of cyclic AMP they stimulated poly(A) RNA ...
12
Efficient and accurate in vitro processing of simian virus 40-associated small RNA.
... Size analysis of viral RNAs produced in isolated nuclei incubated in the presence of either GTP or ITP in the transcription reaction mixture.. Lane GTP shows a standard transcription rea[r] ...
6
Transient Stimulation of Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerase and Histone Acetylation in Human Embryonic Kidney Cultures Infected with Adenovirus 2 or 12: Apparent Induction of Host Ribonucleic Acid Synthesis
... The main increase in apparent host RNA synthesis was preceded by i a relatively early transient stimulation of the DNA-dependent RNA polymerase activity in isolated nuclei, and ii a smal[r] ...
11
The Maize Unstable factor for orange1 Is a Dominant Epigenetic Modifier of a Tissue Specifically Silent Allele of pericarp color1
... nuclei. Moreover, P1-wr sequences exhibit reduced lev- 50 mm Tris-HCl, pH 8.5). Alternatively, nuclei were isolated els of DNA methylation in the presence of Ufo1. Interest- as described by Dorweiler ...
12
Encephalomyocarditis Virus Infection of Mouse Plasmacytoma Cells II. Effect on Host RNA Synthesis and RNA Polymerases
... Hence, solubilized levels of RNA polymerase activity do not necessarily reflect in vivo rates of RNA synthesis in intact cells or in isolated nuclei where the activity of endogenous enzy[r] ...
9
Current status and perspectives in atomic force microscopy-based identification of cellular transformation
... Membrane and cytoskeletal changes were largely accompanied by nuclear transformations, with AFM nanoindentation-based analysis of isolated nuclei of healthy (MCF10A) and mal[r] ...
12
Opening and refolding of simian virus 40 and in vitro packaging of foreign DNA.
... Disruption of SV40 virions by EGTA-DTT at a neutral pH gave nucleoprotein complexes that were more efficient templates for transcription than SV40 minichromosomes isolated from nuclei of[r] ...
8
Asynchronous bidirectional replication of polyoma virus DNA.
... During our studies on replication of polyoma virus in isolated 3T6 cell nuclei Buckler-White and Pigiet, manuscript in preparation and on the replication of polyoma virus DNA 1 or polyom[r] ...
11