Knock down gene expression
Reversible External Control of Gene Expression in an NR1 Knock-in Mouse
... over expression of NRl gene and restores the direct control of the expression of NRl by the native NRl ...the expression of a Cre transgene. This variant should preserve expression of N ...
150
Receptor protein tyrosine phosphatase mu expression as a marker for endothelial cell heterogeneity; analysis of RPTPmu gene expression using LacZ knock in mice
... a gene-trap mouse model of the closely related RPTP κ , the consequent loss of RPTP κ ’s enzymatic activity does not produce any obvious pheno- typic defects (Skarnes et ...
10
Effects of CT-Xp Gene Knock down in Melanoma Cell Lines
... Inducible Gene Expression System (Clontech ...G418-resistant gene. After selection, a clone that presented high levels of expression of the transativator as assessed by the transfection of a ...
11
GmENOD40 Expression Decreased in NORK Knock down Transgenic Soybean Roots
... The formation of symbiotic nodules requires two parallel signaling pathways ： bacterial infection and nodule development[6]. These processes are coordinated in both a spatial and temporal manner to ensure successful ...
9
Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance
... reference gene TaActin (forward primer: 5 ′ ACC TTC AGT TGC CCA GCA AT 3 ′ ; reverse primer: 5 ′ CAG AGT CGA GCA CAA TAC CAG TTG 3 ′ ) and TaGAPDH (forward primer: 5 ′ TGT CTG TGG TGT CAA TGA GAA GGA 3 ′ ; reverse ...
9
Ranking novel cancer driving synthetic lethal gene pairs using TCGA data
... SL gene pair identification methods are far from ...SL gene pair identification was based on the screening of certain criteria rather than training and ...SL gene pairs have been accumulated, and the ...
16
Hsp90β inhibition modulates nitric oxide production and nitric oxide induced apoptosis in human chondrocytes
... Hsp90b is a member of the Hsp90 family of protein chaperones. This family plays essential roles in the fold- ing, maturation and activity of many proteins that are involved in signal transduction and transcriptional ...
11
miR-638 regulates gene expression networks associated with emphysematous lung destruction
... target gene expression with mir-638 ...target gene expression. Expression of the predicted targets are significantly different between controls and inhibitors by two-sided ...
13
Golgi tethering factor golgin-97 suppresses breast cancer cell invasiveness by modulating NF-κB activity
... to knock down golgin-97 gene expression in breast cancer cells and then examined the effects on cell viability, migration and ...invasion. Knock- down efficiency of individual ...
17
Dissecting Wnt/β catenin signaling during gastrulation using RNA interference in mouse embryos
... -catenin knock out (CKO) (Lickert et ...individual expression profiles; however, this precludes the use of biological replicates because of limiting amounts of embryonic ...(het) expression profiles ...
11
Knock-down of Kaiso induces proliferation and blocks granulocytic differentiation in blast crisis of chronic myeloid leukemia
... the expression of two known Kaiso target genes, Wnt11 and β-catenin, using ...human gene or transfected with siRNA to Kaiso or p120ctn either alone or in ...β-catenin gene expression (Figure ...
14
The Ancient Small GTPase Rab21 Functions in Intermediate Endocytic Steps in Trypanosomes
... consequence of secondary effects due to TbRab21 knockdown, with proteins being mislocalized and thus resulting in a “traffic jam” that ultimately affected the completion of cytokinesis. To exclude the possibility of ...
16
Secreted APP regulates the function of full-length APP in neurite outgrowth through interaction with integrin beta1
... APP knock-out mouse hippocampi displayed longer axons after 3 DIV than neu- rons from wild-type brains [17], these authors found that co-culture of wild-type or APP knock-out neurons with APP ...
14
RRM2 is a potential prognostic biomarker with functional significance in glioma
... that knock-down of RRM2 suppressed the growth of glioma cells, and RRM2 may perform as a regulator of cell cycle during G2-M phase ...the expression of cyclin A, cyclin B1, and cyclin D1 ...their ...
11
Phospholipase PLA2G7, associated with aggressive prostate cancer, promotes prostate cancer cell migration and invasion and is inhibited by statins
... Supporting the key role of altered lipid metabolism in prostate carcinogenesis, westernized corn oil containing diet has been shown to enhance cancer progression in mice, whereas thiazolidinediones have been reported to ...
15
Long non-coding RNA HOTAIR promotes glioblastoma cell cycle progression in an EZH2 dependent manner
... the expression of the HOTAIR 5’ domain, the expression of p21, p16, Cyclin D1 and Cyclin E were almost restored to basal ...the expression of the HOTAIR 3’ domain did not change the expression ...
10
Boolean genetic network model for the control of C. elegans early embryonic cell cycles
... To study the dynamical properties of the C. elegans early embryonic cell cycle network, we simulated the changing of each gene or protein ’ s value in the network by the Boo- leans functions at different time ...
13
WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition
... endogenously express WNT5A protein (Fig. 1a). Next, HB2 cells were transiently transfected with two different sequence-specific siRNAs targeting WNT5A (as described in the Methods section) for 48 h, and Western blotting ...
15
Knockdown of the nucleosome binding protein 1 inhibits the growth and invasion of clear cell renal cell carcinoma cells in vitro and in vivo
... NSBP1 expression was upregulated in squa- mous cell carcinoma, metastatic MDA-MB-435HM breast cancer cell line and adenocarcinoma, suggesting that NSBP1 may promote tumorigenesis ...
8
Long non-coding RNA CASC15 promotes melanoma progression by epigenetically regulating PDCD4
... CASC15 expression was increased in melanoma tissues and ...CASC15 knock- down inhibited proliferation, facilitated apoptosis and decreased invasion of melanoma cells, while CASC15 overexpression ...
13