lacZ gene
Isolation of a Marek's disease virus (MDV) recombinant containing the lacZ gene of Escherichia coli stably inserted within the MDV US2 gene.
... Insertion of the lacZ gene into the BglII site of the 4.3-kbp EcoRI-BamHI subfragment of BamHI-A indicated that this site is nonessential for virus replication in cell culture.. MDV DNA [r] ...
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Retrovirus promoter-trap vector to induce lacZ gene fusions in mammalian cells.
... Furthermore, by differential sorting of neomycin-resistant cell populations, clones were isolated in which lacZ expression was induced and repressed in growth-arrested and log phase cell[r] ...
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Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae.
... LACZ gene on a centromere-containing plasmid such that no functional @-galactosidase was produced. This construction also includes another promoterless copy of LACZ, containi[r] ...
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Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production
... the LacZ gene (3039 bp) was inserted into the NS5A C-terminal region of JFH1-AM120, designated ...JFH1-AM120- LacZ was significantly decreased after insertion of LacZ gene into the NS5A ...
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Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors.
... For comparison, the same assay was done also with a virus vtat based on the vector pSC11 5 that also expresses the lacZ gene driven by the promoter of the gene encoding the ilK polypepti[r] ...
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Rapid construction of mycobacterial mutagenesis vectors using ligation independent cloning
... given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a suicide delivery ...a gene ...
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Effects of Genomic Length on Translocation of Hepatitis B Virus Polymerase-Linked Oligomer
... Effects of insertion sizes on polymerase-linked oligomer translocation. To further understand the relevance of the length of the insertion sequence and the acceptor site selection of the polymerase-linked oligomer, ...
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I TRAP: A method to identify transcriptional regulator activated promoters
... sigE gene and 511 bp of the 5' end of the adjacent ORF1 gene was PCR amplified using primer 7208 (which contains a HpaI restriction site) and primer 7209 and cloned into the EcoRV blunt-cloning site of ...
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Proteoglycans secreted by packaging cell lines inhibit retrovirus infection.
... ecotropic lacZ viruses are not ...coli lacZ gene and quantitative assays to measure infection to show that the efficiency of retroviral infection was linearly proportional to virus concentration at ...
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A somatic gene transfer approach using recombinant fusion proteins to map muscle motoneuron projections in Xenopus spinal cord
... somatic gene transfer with fusion protein technology has been developed, thus providing an innovative means of mapping muscle-motoneuronal connections in Xenopus tadpole spinal ...the LacZ gene to ...
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Reticuloendotheliosis type C and primate type D oncoretroviruses are members of the same receptor interference group.
... Superinfection experiments using a vector virus containing an Escherichia coli lacZ gene showed that reticuloendotheliosis and simian oncoretroviruses constitute a single receptor interf[r] ...
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Molecular genetical analysis of the nitrate gene cluster of Aspergillus nidulans
... In vivo lacZ - - ~ -g alactosidase activities of the -transformants indicating that the lacZ gene is under control of nitrate induction/nitrogen metabolite repression similar to the ~iaD[r] ...
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Transcriptional targeting of replication defective adenovirus transgene expression to smooth muscle cells in vivo
... bacterial lacZ reporter gene under the tran- scriptional control of the smooth muscle cell (SMC)-specific SM22 a ...the lacZ reporter gene in primary rat aortic SMCs and immortalized A7r5 ...
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Expression Vectors for Methanococcus maripaludis: Overexpression of Acetohydroxyacid Synthase and β-Galactosidase
... coli lacZ gene was engineered by PCR for ligation was ligated into a SacI-digested pUC18, yielding ...The lacZ product was cloned into pGEM-T, yielding puter Group, Madison, ...the lacZ ...
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Development and Optimization of Herpes Simplex Virus Vectors for Multiple Long-Term Gene Delivery to the Peripheral Nervous System
... long-term gene expression in a rela- tively non-promoter-specific ...drive lacZ and GFP genes, respectively, which can be easily replaced by other genes when ...for gene expression during latency ...
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The human herpesvirus 8 chemokine receptor vGPCR triggers autonomous proliferation of endothelial cells
... We have used a novel conditional transgenic system to study the mechanisms of angioproliferation induced by viral G protein–coupled receptor (vGPCR), the constitutively active chemokine receptor encoded by human ...
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RNA structure and heterologous recombination in the double-stranded RNA bacteriophage phi 6.
... coli lacZAm15 amp PT7 PT3 lacZ' amp lacZ' kan cartridge in PstI site Cartridge with most of lacZ amp lacZ' with pUC8 MCS; vector for pseudomonads amp PT7; exact copy of segment M in pT7T[r] ...
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Activation of a Cell Entry Pathway Common to Type C Mammalian Retroviruses by Soluble Envelope Fragments
... Activation of AdelH retroviruses by A-RBD Cells XC XC XC-A-RBDd XC XC Prebinding conditiona Binding and infection conditionb Titer A-RBD lacZ AdelH lacZ AdelH lacZ AdelH ⫹ A-RBDc lacZ Ad[r] ...
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Circling behavior developed in Dmp1 null mice is due to bone defects in the vestibular apparatus
... Dmp1 lacZ signal, the above tissues were briefly fixed in 4% PFA on ice, followed by sucrose precipitation and frozen section ...a lacZ signal was only detected in some of these muscle cells in a ...
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Adenovirus mediated Cre deletion of floxed sequences in primary mouse cells is an efficient alternative for studies of gene deletion
... We report for the first time the phenotype of deletion of DNA ligase 1 from adult hepatocytes. Homozygous germline deletion of DNA ligase 1 is embryonic lethal (35,36), so conditional gene targeting strategies are ...
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