LAMP Assay
Development and evaluation of a novel LAMP assay for the diagnosis of Cutaneous and Visceral Leishmaniasis
... Development and evaluation of a novel LAMP assay for the diagnosis of Cutaneous and Visceral Leishmaniasis 1 Development and evaluation of a novel LAMP assay for the diagnosis of Cutaneous and Viscera[.] ...
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<p>Multiplex loop-mediated isothermal amplification (multi-LAMP) assay for rapid detection of <em>mcr-1</em> to <em>mcr-5</em> in colistin-resistant bacteria</p>
... This study has some limitations; the sensitivity and speci fi city of the multi-LAMP assay are relatively poorer when applied to samples containing more than one mcr genes. Nonetheless, according to other ...
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Utility of loop-mediated isothermal amplification (LAMP) assay and ELISA in confirmation of leptospirosis: A Pilot study
... rrs LAMP assay which can be used in heated urine ...the assay was 2 and 10 genome equivalents per reaction under heat-denaturing and non-denaturing ...the assay was evaluated in urine of field ...
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The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification (LAMP) Assay for Tuberculosis Diagnosis in Adults with Chronic Cough in Malawi
... (LAMP) assay for diagnosing tuberculosis is a new test with a number of similarities to Xpert1 MTB/RIF: it is mainly intended for use with sputum specimens, and is a nucleic acid amplification test ...[4]. ...
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Comparison of Three Terminal Detection Methods Based on Loop Mediated Isothermal Amplification (LAMP) Assay for Spring Viremia of Carp Virus (SVCV)
... amplification assay under isothermal conditions, which was originally developed by Notomi et ...of LAMP was even 100-fold higher than the PCR-based methods according to the literature (Yu, ...reaction, ...
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Loop Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives
... is LAMP. LAMP is undoubtedly much sensitive than other GMO detection methods; however, obvious shortages of it also emerge along the analysis ...Firstly, LAMP needs at least 4 primers target 6 ...
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Loop mediated isothermal amplification (LAMP): Early detection of Toxoplasma gondii infection in mice
... bp- LAMP assay and 529 bp-nested PCR revealed some dif- ferences in the sensitivity of both the ...bp-LAMP assay showed positive results for all blood samples from infected ...PCR assay ...
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Host Range and Vertical Transmission of Cyprinid herpesvirus 2
... (LAMP) assay, PCR and real-time PCR to detect CyHV-2 in the eggs of diseased Carassius auratus, and specific products could be amplified from the extracted DNAs from eggs of the diseased ...by LAMP ...
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A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification
... improved LAMP assays, such as visual detection of amplified products by add- ing SYBR Green I or hydroxynaphthol blue (HNB), have made it easier to apply in primary clinical settings or for field ...RT-LAMP ...
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Outbreaks of Multidrug Resistant Pseudomonas aeruginosa in Community Hospitals in Japan
... sessing aac(6 ⬘ )-Iae, we designed four primers (FIP, 5 ⬘ -CAA TAC AAA TGT TTT CGG CGC TAC GTC ACT CCA AAA GGC TAC-3 ⬘ ; BIP, 5 ⬘ -TAA ACG ATG AAT TGT GTG GTT GGG TTG GAT GTA GTT CCC AAG TT-3⬘; F3, 5⬘-TCA CAC ATA AAT TTC ...
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Development of a Loop Mediated Isothermal Amplification Method for Detection of Histoplasma capsulatum DNA in Clinical Samples
... via LAMP has several cost advan- tages over ...Further, LAMP requires no thermal cy- cling equipment, since the assay is performed at a single temper- ature, allowing the use of either a heat block ...
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Loop Mediated Isothermal Amplification for Rapid and Reliable Diagnosis of Tuberculous Meningitis
... the LAMP assay was more sensitive than nested ...be LAMP positive but nested-PCR ...these LAMP-positive but nested-PCR-negative samples have a low OD value with LAMP strongly suggests ...
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Use of a Combination of Brushing Technique and the Loop Mediated Isothermal Amplification Method as a Novel, Rapid, and Safe System for Detection of Helicobacter pylori
... pling error (18). Thus, false-negative results in some groups of patients may be due to a relatively low density of bacteria. In the present study, 21 patients were negative by culturing from biopsy materials but ...
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Development and Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid and Real Time Detection of Japanese Encephalitis Virus
... the LAMP method is extremely high due to continuous amplifica- tion under isothermal conditions producing a large amount of target DNA as well as a large amount of the by-product mag- nesium pyrophosphate, leading ...
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Development of loop-mediated isothermal amplification coupled with nanoparticle-based lateral flow biosensor assay for Mycoplasma pneumoniae detection
... We declared that materials described in the manuscript, including all relevant raw data, will be freely available to any scientist wishing to use them for non- commercial purposes, without breaching participant ...
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Original Article Development and validation of a rapid and simple fluorescence real-time loop-mediated isothermal amplification assay for detection of Burkholderia pseudomallei
... Abstract: Background and objective: Melioidosis is a disease caused by Burkholderia pseudomallei, which is a Gram-negative bacterium found in the environment that is distributed across the warm tropical regions. In these ...
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Vol 30, No 1 (2020)
... the LAMP positive amplicons were observed with naked eye by adding fluorescent dye to the reaction ...Hence, LAMP assay has several remarkable advantages over any other colorimetric-based methods and ...
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Development and Evaluation of a Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid Detection of Enterovirus 71
... RT-LAMP assay. Two samples tested negative by the RT-LAMP assay but positive by the rRT-PCR ...RT-LAMP assay is slightly less sensitive than the rRT-PCR assay for ...
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Rapid identification of Aconitum plants based on loop mediated isothermal amplification assay
... Aconitum japonicum Thunb. subsp. subcuneatum (Nakai) Kadota, Aconitum sachalinense F. Schmidt subsp. yezoense (Nakai) Kadota, Aconitum chinense Siebold ex Paxton, Anemone flaccida, and Parasenecio delphiniifo- lius were ...
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Detection of dengue viruses using reverse transcription loop mediated isothermal amplification
... RT-LAMP assay developed here differs from those reported earlier in at least two major areas ...RT-LAMP assay which employed serotype-specific primers targeting the proximal half of the 3′UTR ...
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