laser ionization mass spectra analysis
Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI- TOF MS) is a rapid identification system which is widely used in diagnostic microbiology ...short analysis ...
6
Comparison of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal
... reference spectra were determined for 437 field-caught Culicoides biting midge species from Senegal, including ...with mass ranges of 2 to 20 kDa and high signal ...phylogenetic analysis with TOPALi ...
9
Mass determination of major plasma proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
... the analysis is primarily qualita- tive in ...Qualitative analysis of the global pattern of most abundant proteins may have value for a number of ...MALDI-TOF/MS analysis of serum or plasma may be of ...
13
Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging (IR-MALDESI MSI): Fundamentals, Source Design and Data Analysis.
... proteomic analysis and could alleviate the discrimination against larger molecules (>3000 m/z) that is observed with AP-MALDI ...off-resonance laser desorption with electrospray post-ionization ...
263
Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors
... matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) spectrum database based on the analysis of the legs of six tick vectors: Amblyomma variegatum, ...
7
Differentiation of Streptococcus pneumoniae from Nonpneumococcal Streptococci of the Streptococcus mitis Group by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... reference spectra but seems to result from true limits of phylogenetic resolution of the whole-spectrum similarity measures employed by these systems: in a study by Williamson et ...our analysis of a ...
5
Optimization of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Analysis for Bacterial Identification
... on analysis of unique “fingerprints” of abundant proteins from whole cells or cellular extracts (15, 23, 26, ...reference spectra, and similarity scores for the top database matches are used to determine ...
8
Rapid metabolic profiling of Nicotiana tabacum defence responses against Phytophthora nicotianae using direct infrared laser desorption ionization mass spectrometry and principal component analysis
... tivars of Nicotiana tabacum, the resistant cultivar SNN and the nonresistant cultivar Xanthi, which exhibits no or only a weak HR. In SNN, high JA levels appear transiently during the first hour post infection [70]. The ...
16
Identification of Highly Pathogenic Microorganisms by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial
... MALDI-TOF mass spectrometry is a rapid, accurate, and relatively inexpensive technique that is becoming increasingly impor- tant in microbiological diagnostics to complement classical microbiology, PCR, and ...
9
Advances in Identification of Clinical Yeast Isolates by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... no spectra present for a given genus or species, unknown isolates of that genus or species will produce low scores or unacceptable ...of spectra from rare, non-Candida species ...of spectra obtained ...
8
Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... temperature. Mass spectra were acquired using the MALDI-TOF spectrometer in a linear positive mode (Mi- croflex; Bruker ...Measured mass spectra ranged from 2,000 to 20,000 ...of ...
7
Identification of Rare Pathogenic Bacteria in a Clinical Microbiology Laboratory: Impact of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... MALDI-TOF mass spectrum database previously updated as described ...2 spectra had scores of ⱖ ...MS analysis of 2 additional ...4 spectra was done for unsatisfied species identification in the ...
13
Dermatophyte Identification Using Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... dermatophyte spectra likely enhanced the species-spe- cific diversity covered by the library, which allowed an in- creased capacity of the database to differentiate between subtle intraspecies protein ...MALDI-TOF ...
5
Antifungal Susceptibility Testing of Aspergillus spp by Using a Composite Correlation Index (CCI) Based Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method Appears To Not Offer Benefit over Traditional Broth Microdilution Testing
... LT mass spectrometer system and Bruker MBT Compass Explorer software ...24 spectra were acquired and used to generate the CCI scores used to compare all drug ...the spectra were more similar to that ...
5
Implementation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
... trix-assisted laser desorption ionization–time of flight mass spec- trometry (MALDI-TOF MS) has emerged as a reliable, fast, and cost-effective alternative for bacterial species identification and is ...
7
Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... bacterial mass (approxi- mately 10 l) was suspended in 300 l of Milli-Q water using a mi- cropestle, and the suspension was vortex-mixed for 30 ...rial mass at 100°C for 30 min in a water bath ...
11
Time Dependent Changes of Nicotine and Cotinine In Mouth Cavity After Smoking Studied By MALDI MS Using Functional Organic Analog of Zeolite
... was observed at m/z = 393, and this mass region is almost devoid of noise peaks. In our previous research using inorganic zeolite, we found that zeolite prevented the fragmentation of adsorbed molecules, which in ...
8
Evaluation of a Simple Protein Extraction Method for Species Identification of Clinically Relevant Staphylococci by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... Data analysis. The manufacturer’s recommended log score identifi- cation criteria were used as follows: a score of 2.000 to 3.000 indicated species-level identification, a score of 1.700 to 1.999 indicated ...
5
Anti-biofilm and antiradical activity of different essential oils
... the analysis of the biofilm, the slide and toothpick were washed two times with ultrapure water and bacteria from the glass slide and wooden toothpick were collected with a sterile ...
7
Comparison of analytical profile index, microscan walkaway 40 plus and bruker maldi biotyper systems for identification of enteric pathogens in Kenya
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) also known as Bruker MALDI Biotyper is a technology that allows instant identification of microorganisms by ...
105