Lateral-flow immunoassay
Lateral Flow Immunoassay for Rapid Diagnosis of Potato Blackleg Caused by Pectobacterium Atrosepticum
... Potato blackleg and soft rot caused by Pectobacterium atrosepticum lead to significant yield losses. The early detection of P. atrosepticum is essential for healthy potato seed tubers. The aim of this study was to ...
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Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin
... expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and ...a lateral flow immunoassay ...
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Point of care testing evaluation of lateral flow immunoassay for diagnosis of cryptococcus meningitis in HIV positive patients at an urban hospital in Nairobi, Kenya, 2017
... AFENET: African Field Epidemiology Network; CFR: case fatality rate; CI: confidence interval; CM: cryptococcal meningitis; CMRL: Central Microbiology Reference Lab; CSF: cerebrospinal fluid; LA: latex agglutination; LFA: ...
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Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite
... Abstract: Semiquantitative and rapid detection of specific IgE (sIgE) with well clinical relevance to house dust mite (HDM) are promising for prevalence rhinitis and asthma patients due to the increasing air pollution. ...
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Direct Blood PCR in Combination with Nucleic Acid Lateral Flow Immunoassay for Detection of Plasmodium Species in Settings Where Malaria Is Endemic
... We describe here a sensitive and robust method, db-PCR- NALFIA, for the detection of Plasmodium species directly from blood samples. The standard PCR technology has been simpli- fied by circumventing DNA extraction prior ...
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A two colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line
... Methods: In this work, a two-colour lateral flow immunoassay (LFA) system was developed to identify infections by Plasmodium spp. and differentiate Plasmodium falciparum infection from the other ...
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Management of Factors for Improving Antigen–Antibody Interaction in Lateral flow Immunoassay of Tetracycline in Human Serum Samples
... as lateral flow immunoassay, for the on-site control of ...of lateral flow immunoassay to increase its sensitivity for detecting tetracycline in human serum ...
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Lateral Flow Immunoassay for Diagnosis of Trypanosoma cruzi Infection with High Correlation to the Radioimmunoprecipitation Assay
... a lateral flow immunoassay for the rapid detection of ...and lateral flow immunoassay studies are presented, along with an additional recombinant protein, ...
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Multiplex lateral flow immunoassay: an overview of strategies towards high-throughput point-of-need testing
... Several individual strips can be arranged in a special holder, also designed as to collect one sample and distribute it between several strips. The sample flows in parallel on each strip, which in turn can hold one or ...
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A colloidal gold based lateral flow immunoassay for direct determination of haemoglobin A1c in whole blood
... 3.3.3 Optimization of test lines. To achieve visual inter- pretation of HbA1c levels based on the number and the inten- sity of the test lines shown, the test zone on the NC membrane was expanded to contain three lines ...
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Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR 1 Producing Enterobacteriaceae
... ABSTRACT Colistin has become a last-resort antibiotic for the treatment of infec- tions caused by highly drug-resistant Gram-negative bacteria. Moreover, it has been widely used in the livestock sector. As a consequence, ...
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Dual rapid lateral flow immunoassay fingerstick wholeblood testing for syphilis and HIV infections is acceptable and accurate, Port au Prince, Haiti
... Table 2 Field performance of the SD BIOLINE HIV/Syphilis Duo dual rapid test in Haiti for detection of Treponema pallidum antibodies using a dual HIV/syphilis test, 2014 Treponema pallid[r] ...
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Immediate determination of ACPA and rheumatoid factor a novel point of care test for detection of anti MCV antibodies and rheumatoid factor using a lateral flow immunoassay
... In this study, a mid-range sensitivity and excellent diag- nostic specificity were documented for the simultaneous detection of anti-MCV antibodies and RF-IgG using POCT. As a major technical difference to established ...
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Development and Validation of a Lateral Flow Immunoassay for Rapid Detection of NDM Producing Enterobacteriaceae
... ABSTRACT The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. The NDM-1 carbapenemase is among the ...
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Development of a Nanobody-Based Lateral Flow Immunoassay for Detection of Human Norovirus
... FIG 1 Analysis of Nano-85 binding to clinical norovirus specimens and VLPs. (A) Representative lateral flow strip assay showing the positive signal for norovirus detection. (B) Sensitivities and ...
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Biosensors for fertility and pregnancy in cattle : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry at Massey University, Palmerston North, New Zealand
... and lateral flow immunoassay (LFIA) methods with a new progesterone (P4) sensing material to develop cost effective assays for progesterone sensing in bovine serum and milk ...
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Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis
... acid lateral flow immunoassay in combination with PCR, demonstrates lower or similar detection limits in comparison to gel electrophoresis analysis ...convenient lateral flow ...
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Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed
... Abstract: Although high melamine (MEL) intake has been proven to cause serious health problems, MEL is sometimes illegally added to milk products and animal feed, arousing serious food safety concerns. A satisfactory ...
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DEVELOPMENT OF RAPID ANTIGEN DETECTION TEST FOR METHICILLIN RESISTANT Staphylococcus aureus INFECTION
... Lateral flow immunoassay for methicillin resis- tant staphylococci detection: The current lateral flow assay test strip was based on previously des- cribed system developed by Shin et ...
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Development and Performance Evaluation of an Antibody-Based Technology for Detection of E. coli O157 in Meat Samples and Its Potential Evolution Using Antibody Engineering
... Besides antibody pairing, determining the suitable concentration of antibodies used in the in-tube sandwich immunoassay played a key role in the sensitivity and specificity of the LFIA Test Kit. However, even when ...
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