LENGTH-POLYMORPHISM ANALYSIS
Rapid identification of mycobacteria to species level by PCR restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species
... PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. C. Bo ¨ttger, and T. Bodmer, J. Clin. Microbiol. 31:175–178, 1993) was ...
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Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR Based Restriction Fragment Length Polymorphism Analysis
... A molecular typing approach for Campylobacter jejuni and Campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb PCR-amplified portion of the ...
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Genetic Diversity of Mycobacterium africanum Clinical Isolates Based on IS6110 Restriction Fragment Length Polymorphism Analysis, Spoligotyping, and Variable Number of Tandem DNA Repeats
... Significance of M. africanum genotyping in a probable evo- lutionary scenario for the M. tuberculosis complex. The results obtained demonstrate that phenotypic and/or genotypic tests specifically developed to diagnose M. ...
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Exploration of amplified fragment length polymorphism analysis as an effective tool for discriminating pathogenic strains of bacteria
... AFLP analysis as a routine clinical technique included assessing the discriminatory power of AFLP analysis by comparing closely related strains of a single ...AFLP analysis, it was important to ...
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Differentiation of Burkholderia Species by PCR Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene and Application to Cystic Fibrosis Isolates
... Burkholderia cepacia, first described as the agent of the on- ion soft rot (2), is an opportunistic human pathogen which may be responsible for various nosocomial infections and is espe- cially problematic in patients ...
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Genetic Diversity of Actinobacillus pleuropneumoniae Assessed by Amplified Fragment Length Polymorphism Analysis
... Discriminatory power of AFLP within A. pleuropneumoniae serovar 2. Among the 43 analyzed serovar 2 strains, including the nontypeable serovar-2-like isolate 5779/98, 26 unique AFLP profiles were detected. The ...
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Restriction Fragment Length Polymorphism Analysis of Some Flagellin Genes of Salmonella enterica
... Salmonella isolates expressing two antigenically distinct types of flagellin are biphasic. Monophasic Salmonella strains expressing only one type of flagellar antigen include many clinically and epidemiologically ...
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Cleavase Fragment Length Polymorphism Analysis of Neisseria meningitidis Basic Metabolic Genes
... ated from the outbreak-associated strains by both glnA and carA. Two additional sporadic-case isolates (M393 and M4594) could be differentiated from the outbreak-associated strains by glnA. Isolate M393 also had a ...
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Genotyping of Epidemic Methicillin ResistantStaphylococcus aureus Phage Type 15 Isolates by Fluorescent Amplified Fragment Length Polymorphism Analysis
... Fragment analysis. Fragments were sized with GeneScan version 2.1 software. Peak height thresholds were set at 50; any peak heights less than this value were not included in the analysis. Electropherograms ...
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Amplified Fragment Length Polymorphism Analysis: the State of an Art
... gel analysis software like GelCompar soft- ware (Applied Maths, Kortrijk, ...for analysis by the dedicated ABI GenoTyper ...cluster analysis, which is essential for epidemiological and phylogenetic ...
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Restriction fragment length polymorphism analysis of clinical isolates of Mycobacterium haemophilum
... Among New York patients, the type 2 clone was isolated from skin 11 cases, bone 1 case, blood 1 case, lung 2 cases, and joint 1 case, and 9 of 15 patients with the type 2 clone had AIDS.[r] ...
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Genotyping Neisseria gonorrhoeaeUsing Fluorescent Amplified Fragment Length Polymorphism Analysis
... FAFLP is a rapid, highly discriminatory typing tool that is able to distinguish epidemiologically related from unrelated isolates of N. gonorrhoeae. It generates reproducible, accu- rately sized fragment patterns that ...
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Molecular Identification of Cryptococcus neoformans Serotypes
... Cryptococcus neoformans is a fungal pathogen that causes life-threatening infections primarily in immunocom- promised hosts. Based on the genetic characteristics and serologic properties of capsular polysaccharides, ...
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Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... sequence analysis (3, 4, 15–17, 19, 26, 31, 32), can differentiate mycobacteria to the species level, but these techniques are labor intensive and difficult to perform for routine use in clin- ical ...fragment ...
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Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex
... Differentiation of slowly growing Mycobacterium species including Mycobactenium tuberculosis, by gene amplification and restriction fragment length polymorphism analysis.. Identification[r] ...
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Single Nucleotide Polymorphisms in the IS900 Sequence of Mycobacterium avium subsp paratuberculosis Are Strain Type Specific
... fragment length polymorphism analysis with hybridization to IS900 (19), pulsed-field gel electrophoresis (PFGE) (11, 29), PCR-restriction enzyme analysis (PCR-REA) of gyrB (5), PCR-REA of inhA ...
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Examining Complex Microbial Communities Using the Terminal Restriction Fragment Length Polymorphism Method and Dedicated TRFLP Analysis Software
... Fragment Length Polymorphism analysis could be effectively used to examine microbial communities associated with swine waste lagoon systems (Devine, Chapter ...TRFLP analysis enabled the ...
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Algorithmic Approach to High Throughput Molecular Screening for Alpha Interferon Resistant Genotypes in Hepatitis C Patients
... fragment length polymorphism analysis of the 59 UTR (7) that is able to dis- criminate types 1a, 1b, 2a, 3a, 3b, 4, 5, and 6, the allele-specific oligonucleotide analysis such as the LiPA ...
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Molecular Fingerprinting of Mycobacterium tuberculosisand Risk Factors for Tuberculosis Transmission in Paris, France, and Surrounding Area
... Cluster analysis. Table 1 presents the characteristics of the patients and strains in 10 clusters each containing four or more patients. Nosocomial transmission was considered for one pa- tient in cluster A who ...
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Molecular Diversity of Methicillin-resistant Staphylococcus aureus Isolates Originated from Patients in Ahvaz Hospitals, Iran
... molecular analysis of four ...RFLP analysis using the AluI, HindIII, TaqI and RsaI restriction ...the analysis of coa gene, 8 types, spa gene 5 types and 17 subtypes, coa gene with AluI 13 types, and ...
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