Lethal Mutagenesis
Lethal Mutagenesis of Foot-and-Mouth Disease Virus Involves Shifts in Sequence Space
... Lethal mutagenesis or virus transition into error catastrophe is an antiviral strategy that aims at extin- guishing a virus by increasing the viral mutation rates during ...of lethal ...
14
Contribution of a Multifunctional Polymerase Region of Foot-and-Mouth Disease Virus to Lethal Mutagenesis
... extinguished earlier than FMDV C-S8c1 by ribavirin, its mutant spectrum in 3D re- mained less complex than that of the wild-type virus. This difference suggests either some limitation of FMDV-3D(M16A) to accept mutations ...
17
T-705 (Favipiravir) Induces Lethal Mutagenesis in Influenza A H1N1 Viruses In Vitro
... Several novel anti-influenza compounds are in various phases of clinical development. One of these, T-705 (favipiravir), has a mechanism of action that is not fully understood but is suggested to target influenza virus ...
11
Effective Lethal Mutagenesis of Influenza Virus by Three Nucleoside Analogs
... Lethal mutagenesis is a broad-spectrum antiviral strategy that exploits the high mutation rate and low mutational tolerance of many RNA ...of lethal mutagenesis as a strategy against influenza ...
14
5-Azacytidine Can Induce Lethal Mutagenesis in Human Immunodeficiency Virus Type 1
... support lethal mutagenesis as its mechanism of ac- ...to lethal mu- tagenesis that is characterized by a significant increase in tran- sition mutations within the ...
9
Lethal Mutagenesis of Poliovirus Mediated by a Mutagenic Pyrimidine Analogue
... Lethal mutagenesis is the mechanism of action of ribavirin against poliovirus (PV) and numerous other RNA ...and mutagenesis, presumably a reflection of rP nucleotide formation by a nucleotide ...
11
Empirical Complexities in the Genetic Foundations of Lethal Mutagenesis
... At face value, the virion survival methods developed in this early work are ideally suited to test the foundations of lethal mutagenesis theory, because they seem to provide genome survival rates that could ...
12
Theory of Lethal Mutagenesis for Viruses
... Yet, even this simple protocol, which is technically feasible with many viruses, gives a direct estimate of mutation rate only if viral replication within a cell does not select against muta- tions that arise within that ...
10
Mutational Robustness of an RNA Virus Influences Sensitivity to Lethal Mutagenesis
... The suggestion that RNA viruses obey the strictures of lethal mutagenesis theory has significant implications for antiviral ther- apy. Subtle ( ⬍ 2 log) decreases in virus titer may be sufficient to reduce ...
5
Inhibitory Effect of a Nucleotide Analog on Infectious Salmon Anemia Virus Infection
... The experiment conducted in order to determine the mech- anism by which ribavirin inhibit the replicative cycle of ISAV, showed the following. (i) Guanosine did not reverse the inhi- bition of ribavirin, and ribavirin ...
9
Genetic Characterization of Cytological Region 77A–D Harboring the Presenilin Gene of Drosophila melanogaster
... one lethal complemen- tants mapping to the 77B/C region were unsuccessful, tation group as Psn gene mutations (Ye et ...systematic lethal mutagenesis in this article we identify two additional ...
9
Gene Deletion by Fluorescence Reported Allelic Exchange Mutagenesis in Chlamydia trachomatis
... the mutagenesis rate was unknown, both antibiotic selection and fluorescence were included in order to discern true homologous recombinants which have exchanged trpA with the cassette from spontaneously resistant ...
9
CRISPR/Cas9 Mutagenesis in Phlebotomus papatasi: the Immune Deficiency Pathway Impacts Vector Competence for Leishmania major
... fly mutagenesis have been published so far, and gene downregula- tion by small interfering RNA (siRNA) is possible but technically challenging, as it requires microinjection and the survival of adult females ...
14
Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps
... of lethal lines and developmental phenotypes We analyzed the embryonic cuticle phenotypes of many lines identified as lethal and found a number of distinct cuticular abnormalities (Figure ...many ...
12
An efficient one step site directed deletion, insertion, single and multiple site plasmid mutagenesis protocol
... In our structural proteomics lab, site-directed mutagene- sis, truncation and deletion mutagenesis are routine methods used by variety of personnel with different exper- tise in molecular biology. Although most ...
10
Potential Test of Papaya Leaf and Seed Extract (Carica Papaya) as Larvicides against Anopheles Mosquito Larvae Mortality. Sp in Jayapura, Papua Indonesia
... In this study, Lethal Concentration values calculated at two time periods, the period of time of 12 hours LC50 values obtained at 120.987 ppm and LC90 values in ppm 1080.689. In the period of 24 hours LC50 values ...
8
HYPOMORPHIC LETHAL MUTATIONS AND THEIR IMPLICATIONS FOR THE INTERPRETATION OF LETHAL COMPLEMENTATION STUDIES IN DROSOPHILA
... We refer to these mutations as “haplo-specific lethal mutations.” In studies of autosomal lethals, haplo-specific lethal mutations can be included in lethal com- plementation [r] ...
12
GENETIC STUDIES ON DROSOPHILA SIMULANS. II. SEX-LINKED GROUP OF GENES
... The lethal (really semi-lethal) effect and the dwarf character are evidently due to the same gene, which is conveniently referred to as “dwarf,” rather than “lethal,” the orig[r] ...
22
Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer
... In summary, the development and characterization of a transposon system that is functional in Chlamydia represent a substantial advance and a valued addition to the growing repertoire of genetic manipulations in this ...
24
Fast Neutron Mutagenesis in Plants: Advances, Applications and Challenges
... neutron mutagenesis was used to study forward genetics in plants but, now it has also been applied in reverse genetics studies ...neutron mutagenesis is Deletagene (Delete-a-gene) ...neutron ...
24