limulus amoebocyte lysate
AN OVERVIEW OF LIMULUS AMOEBOCYTE LYSATE (LAL) TEST
... Limulus Amoebocyte Lysate (LAL) test is in vitro test to detect and measure bacterial endotoxins. It is the most critical quality control test required by FDA for all parenteral drugs in their final ...
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Comparison of plasma extraction techniques in preparation of samples for endotoxin testing by the Limulus amoebocyte lysate test
... Comparison of Limulus amoebocyte lysate test results from healthy human plasma samples extracted by these two methods indicated that lysate type and not extraction procedure was associat[r] ...
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Chromogenic Limulus amoebocyte lysate assay for rapid detection of gram negative bacteriuria
... These data suggest that chromogenic Limulus amoebocyte lysate assay of urine has potential usefulness as a rapid, reliable, and easily performed and interpreted screening test for the di[r] ...
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Interaction between limulus amoebocyte lysate and soluble antigens from Pseudomonas aeruginosa and Staphylococcus aureus studied by quantitative immunoelectrophoresis
... To investigate the interaction of Limulus amoebocyte lysate LAL with gram-negative bacteria, soluble antigens from sonicated Pseudomonas aeruginosa were studied by various crossed-immuno[r] ...
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Turbidimetric method for quantifying serum inhibition of Limulus amoebocyte lysate
... When the inhibition of LPS in the presence of serum was compared by using different types of LPS, the more potent LPS types determined in saline were inhibited to a greater degree than t[r] ...
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Rapid detection of Gram negative bacteriuria by Limulus amoebocyte lysate assay
... Four urine samples containing between 5 x 104 and 105 gram-negative bacteria per ml can be diluted and still yield a positive LAL assay within the 15-min incubation time; however, diluti[r] ...
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Statistical determination of endotoxin content in influenza virus vaccine by the limulus amoebocyte lysate test
... The variability of the log2 ratio of the endotoxin content of an influenza virus vaccine and reference E-1 was estimated in this collaboraRESULTS tive study by the SD pooled over four va[r] ...
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New, sensitive rocket immunoelectrophoretic assay for measurement of the reaction between endotoxin and Limulus amoebocyte lysate
... Antigens, 5 IlI of the diluted LAL plus CSF mixtures was applied to the wells; antibody, polyspecific antibody against LAL in the whole gel 7.5 jll/cm2; control, from a patient suspected[r] ...
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BACTERIAL ENDOTOXIN TEST FOR CERTAIN PHARMACEUTICAL COMPOUNDS
... LAL (limulus amoebocyte lysate) is developed with the innovative work of john Hopkins university investigators. Frederick bang observed that bacteria caused intravascular coagulation in the American ...
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Application of a Limulus test device in rapid evaluation of gonococcal and nongonococcal urethritis in males
... Thus, use of the Limulus amoebocyte lysate test device would enable the private physician to make an accurate, presumptive diagnosis of gonococcal and nongonococcal disease in males with[r] ...
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Airborne Endotoxin from Indoor and Outdoor Environments: Effects of Sample Dilution on the Kinetic Limulus Amebocyte Lysate (LAL) Assay.
... Airborne endotoxins in occupational environments are a potential respiratory hazard to individuals. In this study, air- borne endotoxins were collected using open-face and button aerosol samplers from inside animal ...
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Immunization of mice with soluble lysate of interferon gamma expressing Plasmodium berghei ANKA induces high IFN-γ production
... TPbA lysate containing 35 pg/ml IFN-γ (200 μl) at 7 days ...Soluble lysate of plain RBC (Group 3) and WT PbA parasite and its lys- ate (Group 4) were used in the control ...
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Cleavage of a viral polyprotein by a cellular proteolytic activity.
... The 200K polyprotein was synthesized in the reticulocyte lysate in the absence of DTT, and then the reaction mixtures were serially diluted either with a blank lysate translation solutio[r] ...
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Origin and Rapid Evolution of a Novel Murine Erythroleukemia Virus of the Spleen Focus-Forming Virus Family
... Lane 1, spleen lysate from a control animal infected with ecotropic helper virus; lane 2, spleen lysate from infected day 67 mouse with massive splenomegaly Table 1; lane 3, cell lysate [r] ...
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The Ability of Limulus to See Visual Patterns
... When the animals entered the experimental compartment they could see a moving pattern which consisted of vertical light and dark stripes.. The stripe pattern was projected on the circula[r] ...
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Neutralizing determinants defined by monoclonal antibodies on polypeptides specified by bovine herpesvirus 1.
... MCA were screened by the ELISA with purified BHV-1, purified BHV-1 nucleocapsid, BHV-1infected cell lysate, herpes simplex virus type 2-infected cell lysate, and noninfected cell lysate [r] ...
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Some Observations on the Haemocyanin Of Limulus
... 210, "to predict the likelihood on the basis of experiments on the haemocyanins of Helix and Crustacea, that with more extended observation, the haemocyanin of Limulus will not be found [r] ...
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Proprioception in Limulus
... Oscillograms from Limului small leg nerve, showing impulses in fibres from proprioceptor neurons at the femoro-tibial joint, a, joint partially flexed; 6, joint allowed to flex fully und[r] ...
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Determination of endotoxin in injectable antibiotic preparations by the chromogenic assay method using a Limulus reagent (Tachypleus hemocyte lysate) and a chromogenic substrate
... In this study, we chose the chromogenic reaction as a method mo?re quantitative and sensitive than the Limulus gelation reaction, and we tried to remove unfavorable factors by ti followi[r] ...
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New microassay for quantitation of endotoxin using Limulus amebocyte lysate combined with enzyme linked immunosorbent assay
... A combined method of the Limulus amebocyte lysate LAL test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigen[r] ...
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