Line blot assay
Nonisotopic Detection and Typing of Human Papillomavirus DNA in Genital Samples by the Line Blot Assay
... by line blot assay by avoiding coampli- ...the line blot ...the line blot assay, 10 copies of HPV-18 scored negative in two of five PCR runs (data not ...the ...
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Genotyping of Human Papillomavirus in Liquid Cytology Cervical Specimens by the PGMY Line Blot Assay and the SPF10 Line Probe Assay
... When multiple HPV genotypes are present in a sample, the situation becomes more complex. Besides the preferential use of a type-specific subset of PCR primers, competition between the different HPV genotypes may play an ...
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Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR Based Reverse Line Blot Assay
... established sPCR methods, which had been shown to be spe- cific; and avoided contamination by the use of appropriate negative and no-DNA controls. There were relatively few dis- crepancies between methods, and we believe ...
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Detection of Rickettsia in Rhipicephalus sanguineus Ticks and Ctenocephalides felis Fleas from Southeastern Tunisia by Reverse Line Blot Assay
... Species in the Rickettsia genus are divided into two groups: the spotted fever group (SFG) and the typhus group (TG). These ill- nesses are caused by about 22 species. Mediterranean spotted fever (MSF) is caused by ...
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Reverse Line Blot Assay for Direct Identification of Seven Streptococcus agalactiae Major Surface Protein Antigen Genes
... mPCR/RLB assay is an appropri- ate method for epidemiological typing, especially when combined with other molecular markers, as in our three-com- ponent system ...immunological assay, such as a Western ...
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Nonisotopic Detection of Human Papillomavirus DNA in Clinical Specimens Using a Consensus PCR and a Generic Probe Mix in an Enzyme Linked Immunosorbent Assay Format
... immunosorbent assay format to screen for the presence of human papillomavirus (HPV) DNA amplified from clinical ...generic assay is performed, HPV DNA-positive samples can be directly genotyped using a ...
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Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens
... Residual ThinPrep (Cytyc Corporation, Boxborough, MA) cervical samples previously tested for HPV using the Roche line blot assay [9] were used in this study. Twenty samples containing 24 of the 27 ...
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Use of PGMY Primers in L1 Consensus PCR Improves Detection of Human Papillomavirus DNA in Genital Samples
... the line blot assay (PGMY-line blot), while HPV amplicons obtained with the degenerate primer pool MY09/MY11/HMB01 were detected with type-specific radiolabeled probes in a dot ...
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Optimal Threshold for a Positive Hybrid Capture 2 Test for Detection of Human Papillomavirus: Data from the ARTISTIC Trial
... positive samples were retrospectively tested using the prototype Line Blot Assay (LBA; 119. kindly donated by Roche)[r] ...
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Performance of Commercial Reverse Line Blot Assays for Human Papillomavirus Genotyping
... the assay; and (iii) commercial availability in the United ...genotyping assay (LA) (Roche Diag- nostics, Indianapolis, IN), INNO-LiPA HPV Genotyping Extra (Innoge- netics, Ghent, Belgium), and the reverse ...
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New Method for Simultaneous Species Specific Identification of Equine Strongyles (Nematoda, Strongylida) by Reverse Line Blot Hybridization
... reverse line blot (RLB) hybridization assays proved to be powerful for the identification of a diverse range of clinically relevant human and veterinary pathogens, with high throughput (14, 40, 42, 44, ...
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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... based-reverse line blot hybridization (mPCR/RLB), were used to assess 7 common neonatal meningitis bacterial pathongens, including Escherichia coli, Staphylococcus aureus, Listerisa monocytogenes, Neisseria ...
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Validation of western blot for Histoplasma capsulatum antibody detection assay
... western blot performed according to the protocol previously established ...Trans Blot cell (Bio-Rad), membranes were sliced vertically, and free binding sites in the mem- branes were blocked by incubation ...
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Evaluation of a Treponema pallidum western immunoblot assay as a confirmatory test for syphilis
... The results shown in Table 3 indicate that the Western blot assay and FTA-ABS DS were in close agreement, except for the following discordant results: the Western blot assay detected all[r] ...
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Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription PCR Based Reverse Line Blot Hybridization Assay
... microarray assay to detect and differentiate EV71 and CVA16 (4), molec- ular characterization of PV strains by RT-PCR–restriction fragment length polymorphisms (2), and an integrated micro- RT-PCR system for ...
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Rifampin Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis
... the assay RIOT, which stands for rifampin-isoniazid oligonucleotide typing because it is based on reverse line blot DNA hybridization for the simul- taneous detection of mutations in genes rpoB, ...
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Detection and Identification of EightTrichinella Genotypes by Reverse Line Blot Hybridization
... RLB assay (11, 27) to identify and differ- entiate eight different Trichinella genotypes based on a single PCR ...RLB assay is based on variations in the DNA sequences between 5S rDNA genes within the genus ...
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PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis
... new assay that can detect and identify a panel of the most relevant bacterial and fungal pathogens is needed, as broad-range pathogens are increasingly linked to bloodstream ...new assay that can ...
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Comparison of a Multiplexed Herpes Simplex Virus Type-Specific Immunoglobulin G Serology Assay to Immunoblot, Western Blot, and Enzyme-Linked Immunosorbent Assays
... immunoblot assay due to the inclusion of a common HSV (not type specific) antigen ...plex assay, and one was gG-2 positive by the ...multiplex assay, one was also HSV-1 positive by Western blotting, ...
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Detection and enumeration of toxin producing Pasteurella multocida with a colony blot assay
... A Peroxidase-labeled MAb detection of toxin-producing strain P-4533B colonies grown for 16 h on blood agar and transfered to a nylon 66 membrane; P-4533B colonies were in a mixed culture[r] ...
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