Live-cell Imaging
Comparison and optimization of CRISPR/dCas9/gRNA genome labeling systems for live cell imaging
... with live cell ...dCas9/gRNA imaging is to label genomic loci with low- or nonrepetitive regions with a minimal number of tar- geted dCas9/gRNA ...
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Live-cell imaging with genetically encoded protein kinase activity reporters.
... its cell-to-cell ...and live- cell imaging techniques enable us to visualize kinase activity in living cells with high spatial and temporal ...
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Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy
... as live cell imaging for the study of asbestos in this ...is live-cell imaging incorporating fluorescence microscopy ...and live-cell imaging in conjunction ...
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Live cell imaging reveals marked variability in myoblast proliferation and fate
... employed live cell imaging to track myoblast prolifer- ation and monitor survival during a differentiation time ...tomated cell counting algorithm (Figure ...in cell number with an ...
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Optimisation of the synthesis and modification of CdTe quantum dots for enhanced live cell imaging
... excellent live cell imaging agents as they exhibit strong luminescence and excellent photostability while maintaining cell viability over a number of extended incubation ...the cell ...
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Phosphorescent cationic iridium(III) complexes dynamically bound to cyclodextrin vesicles : applications in live cell imaging
... in live biological specimens such as cells, has greatly benetted from the introduction of uorescence-based imaging methods together with tools to deliver such uorescence probes into ...in live ...
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Mechanism of induction of binucleated cells by multiwalled carbon nanotubes as revealed by live-cell imaging analysis
... confocal live-cell imaging system to elu- cidate the mechanism involved in the MWCNT-induced formation of binucleated cells using dichromatically vi- sualized human cells in which chromosomes and ...
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SCOPE Seq: a scalable technology for linking live cell imaging and single cell RNA sequencing
... from live cell imaging data ...monochrome live cell im- aging data (Additional file 1: Supplementary Methods, ...our imaging data and attempted to identify multiplets by manually ...
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A live cell imaging system for visualizing the transport of Marburg virus nucleocapsid like structures
... We previously developed a live-cell imaging system visualizing intracellular transport of NCLSs in EBOV proteins-expressing cells [25]. According to this, the transport characteristics of EBOV NCLSs, ...
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Functional Characterization of Bovine Viral Diarrhea Virus Nonstructural Protein 5A by Reverse Genetic Analysis and Live Cell Imaging
... this cell suspension was then seeded immediately postelectroporation ...For live cell imaging, RNA transcripts (5 g) were electroporated alone or together with peGFP-N1-Perilipin (5 g) into ...
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Live cell imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteins
... expand live cell imaging tools using a novel approach based on zinc finger-DNA recognition ...that live cell imaging of specific DNA sequences can be achieved with artificial ...
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High content live cell imaging for the discovery of new antimalarial marine natural products
... this live cell-imaging assay improves the spatial and temporal resolution of the malaria parasite screening assay, and provides valuable information about the health of host cells under drug ...
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Resolving the Assembly State of Herpes Simplex Virus during Axon Transport by Live-Cell Imaging
... The live-cell imaging data reported here support findings in TEM studies in which HSV capsids enveloped and resident in the neuronal secretory pathway within axons were observed (13, 26, 31, 33, 37, ...
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Facile green synthesis of fluorescent carbon quantum dots from citrus lemon juice for live cell imaging
... Citric acid is an excellent precursor to synthesize the CQDs due to its low carbonization temperature [19,20]. It is well known that the sour test of lemon juice is due to presence of acids of which the major is citric ...
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Practical three color live cell imaging by widefield microscopy
... 2: Live cell Imaging over Time of RGB Fluorescent Proteins That Can form Bright ...color imaging using a triple cube filter set for DAPI/FITC/Texas Red (Semrock DA/FI/TX-B) and switching only ...
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Application of black phosphorus nanodots to live cell imaging
... Fig. 4, the BP nanodots were successfully internalized into C2C12 myoblasts, concentrated in their cytoplasm, and exhibited a specific green fluorescence without undesir- able cytotoxic effects. These results implied ...
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Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo
... T cell infusion for each tested condition was measured (Figure ...T cell adhesion to the inflamed BBB was only transient, as at the later times Table 1 Parameters analyzed by intravital microscopy (modified ...
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The development of quantitative live cell imaging techniques and their applications in the study of inter-cellular communication and sarcoma cell motility
... of cell death was determined by the subsequent uptake and accumulation o f propidium within cell ...then cell nuclei would remain red throughout the entire recording ...NEH cell death is ...
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Live-Cell Imaging and Measurement of Intracellular pH in Filamentous Fungi Using a Genetically Encoded Ratiometric Probe
... ratio imaging, we were unable to demonstrate the presence of either pronounced tip- focused, longitudinal cytoplasmic pH gradient or cytoplasmic pH gradient across the apical 20- m region of actively growing ...
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The roles of kinetochore of micronucleus in mitosis of HeLa cells: a live cell imaging study
... Mitosis is conventionally divided into four stages—pro- phase, metaphase, anaphase, and telophase in an animal cell. During the time-lapse observation, the time points of the four stages of mitosis were recorded ...
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