LMP2 and 7
Identification of a TAP-Independent, Immunoproteasome-Dependent CD8+ T-Cell Epitope in Epstein-Barr Virus Latent Membrane Protein 2
... expressing LMP2 via a recombinant vaccinia virus ...Interestingly LMP2 was not pro- cessed to generate the FLY epitope in either T2 or T3 cells, yet both target lines pulsed with the cognate peptide epitope ...
5
A Novel Diagnostic Marker: Proteasome LMP2/1i-Differential Expression in Human Uterus Mesenchymal Tumor
... survival rate is approximately 35% [5]. However, develop- ing an efficient adjuvant therapy is expected to improve this. Uterine LMA may occur in as many as 70% ~ 80% of women by the age of 50 years [6]. Distinguishing ...
6
CD8+ T cell autoreactivity to an HLA B27–restricted self epitope correlates with ankylosing spondylitis
... sequence-related LMP2 236-244 peptide induces IFN- γ production in both groups (Figure 3b), although, on average, the responses are more pronounced in the group of patients with ...
8
Epigenetic Deregulation of the LMP1/LMP2 Locus of Epstein-Barr Virus by Mutation of a Single CTCF-Cohesin Binding Site
... CTCF and cohesins have been implicated in DNA loop inter- actions (22, 54, 55). Long-distance DNA interactions are essential to mediate communication between promoter and enhancer reg- ulatory elements. EBV OriP has been ...
11
Subversion of immunoproteasome subunit expression in dengue virus serotype 2 infected HepG2 cells
... both LMP2 and LMP7 ...both LMP2 and LMP7 was observed when virus replication was impeded by the antiviral compound ...2; LMP2: low molecular weight polypeptide-2; LMP7: low molecular weight ...
5
Immune reconstitution of HLA A*0201/BMLF1 and HLA A*1101/LMP2 specific Epstein Barr virus cytotoxic T lymphocytes within 90 days after haploidentical hematopoietic stem cell transplantation
... Factors associated with a high risk of EBV-PTLDs include HLA mismatch, EBV serology donor/recipient mismatch (donor+/recipient–), grade II–IV acute graft-versus-host disease (aGVHD), pre-transplant splenectomy, and ...
11
The Proteasome Subunit LMP2/b1i In the Female Genital System
... of LMP2/β1i and LMP7/β5i into the 20S proteasome is responsible for antigen presentation, and inhibiting proteasome activity affected the capacity of cells to present antigenic peptides ...by LMP2/β1i and ...
6
The Intersection of Epstein-Barr Virus with the Germinal Center
... and LMP2 have been shown to possess, respectively, the CD40 and BCR signaling functions necessary for GC survival (7, 8, 13, 15, 35, 47), and EBNA1 is required for replication of the viral ...
9
Analysis of a structured intronic region of the LMP2 pre mRNA from EBV reveals associations with human regulatory proteins and nuclear actin
... EBV (Epstein–Barr virus) is a ubiquitous human herpes virus that infects ~ 95% of adults [1]. EBV is implicated in various cancers [2–4] and autoimmune diseases [5]. Host-virus interactions are crucial to infection and ...
6
Adoptive T-Cell therapy as treatment for Epstein Barr virus-associated malignancies : strategies to enhance potential and broaden application
... the LMP2 peptide library used in this study was designed to identify HLA class I restricted epitopes with a length of 9-11 amino acids, an HLA class II restricted epitope was identified in one of the patient CTL ...
22
Deletion of DNA encoding the first five transmembrane domains of Epstein-Barr virus latent membrane proteins 2A and 2B.
... The last seven transmembrane and carboxy-terminal cytoplasmic domains of Epstein-Barr virus latent membrane 2 LMP2 are dispensable for lymphocyte infection and growth transformation in v[r] ...
7
EBNA-LP Associates with Cellular Proteins Including DNA-PK and HA95
... interaction with EBNA-2 for promoter-specific transcriptional effects (16, 49). EBNA-2 has two essential domains (6, 7). One interacts with cellular, sequence-specific, DNA binding pro- teins, including RBP-J ...
7
A second Epstein-Barr virus membrane protein (LMP2) is expressed in latent infection and colocalizes with LMP1.
... However, the documentation of LMP2 expression in almost all cells in latently infected lymphoblastoid cell lines is a necessary step in the delineation of the association of LMP2 with la[r] ...
8
Ab Initio Studies of the Reaction of Hydrogen Transfer from DNA to the Calicheamicinone Diradical
... the LMP2 method (local Moller-Plesset of second order) (16,17) is applied, as implemented by the Titan program , also using the 6- 31G* basis ...and LMP2 levels for the species featuring ...
6
Distribution, characterization, and induction of CD8+ regulatory T cells and IL 17 producing CD8+ T cells in nasopharyngeal carcinoma
... LMP1, LMP2, EBNA1, and ...and LMP2 (LLW, GLG). From the CD8 high cell population, LMP1 (YLQ) and LMP2 (LLW, GLG) antigen-specific CD8 + T cells and CD8 + Foxp3 + Tregs (Tcregs) were isolated from ...
10
Epstein-Barr virus nuclear antigen 3C is a transcriptional regulator.
... The EBNA-3C transactivation domain is not responsible for EBNA-3C-mediated inhibition of EBNA-2 responsiveness. One possible explanation for the EBNA-3C-mediated inhibi- tory effect on EBNA-2 is that these proteins ...
7
An Epstein-Barr virus protein associated with cell growth transformation interacts with a tyrosine kinase.
... In lanes 1 to 11, in vitro kinase reactions with LMP2, P-Tyr, or control antibody immunoprecipitates from BJAB cells transfected with pSG5, pLMP2A, or LMP2A deletion mutants described in[r] ...
12
HLA A2.1-restricted cytotoxic T cells recognizing a range of Epstein-Barr virus isolates through a defined epitope in latent membrane protein LMP2.
... Effector CTL preparations from donor 1, in which the only EBV-specific reactivity detectable was against the LMP2 residue 426 to 434 epitope, were tested on a panel of LCL targets from t[r] ...
8
MicroRNAs of Epstein-Barr Virus Control Innate and Adaptive Antiviral Immunity
... EBV was the first virus found to encode miRNAs (4, 5) and is the largest reservoir of miRNAs among human herpesviruses known to date. EBV encodes at least 44 miRNAs, which can potentially regulate hundreds of genes, but ...
6
Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts.
... Total RNA from these cells was analyzed by reverse transcription and PCR amplification by using the following specific combinations of oligonucleotide primers: LMP1 mRNA, 5' 2.8 primer a[r] ...
9