Locked nucleic acid (LNA)
Low Percentage of KRAS Mutations Revealed by Locked Nucleic Acid Polymerase Chain Reaction: Implications for Treatment of Metastatic Colorectal Cancer
... a locked nucleic acid (LNA) polymerase chain reaction (PCR), capable of detecting KRAS-mutated alleles at extremely low fre- quency (detection sensitivity limit ...the LNA-PCR ...
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Reliable allele detection using SNP-based PCR primers containing Locked Nucleic Acid: application in genetic mapping
... Single nucleotide polymorphisms (SNPs) represent the most common variations across a genome [1], they occur at a frequency of about one SNP in 1000 nucleotides in genomic DNA [2] and they can be used to directly detect ...
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Simultaneous Detection and Differentiation of Human Rhino and Enteroviruses in Clinical Specimens by Real Time PCR with Locked Nucleic Acid Probes
... of locked nucleic acid (LNA) analogues in short double-dye probes which contained only two selec- tively HRV- or HEV-specific ...the LNA probes in a tricolor real-time PCR assay to ...
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Use of a Locked Nucleic Acid Oligomer in the Clamped Probe Assay for Detection of a Minority Pfcrt K76T Mutant Population of Plasmodium falciparum
... cause it is well tolerated and available at a very low cost. Over the last three decades, P. falciparum chloroquine resistance has significantly increased, and many countries have discarded this drug from the ...
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Validation of a locked nucleic acid based wild-type blocking PCR for the detection of EGFR exon 18/19 mutations
... a LNA based PCR/sequencing method to detect G719A/S/C mu- tations, exon 19 deletions and exon 20 ...sensitive locked nucleic acid (LNA) probe complementary to the wild-type ...sequence. ...
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Rational design of short locked Nucleic Acid-Modified 2′- O -Methyl antisense oligonucleotides for efficient exon-skipping in vitro
... Locked nucleic acid is a prominent nucleic acid analog with un- precedented target binding affinity to cDNA and RNA oligonu- cleotides and shows remarkable stability against nuclease ...
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Development and Evaluation of Novel Real Time Reverse Transcription PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human Pathogenic Coronaviruses
... 35. Thanh TT, Pawestri HA, Ngoc NM, Hien VM, Syahrial H, Trung NV, van Doorn RH, Wertheim HF, Chau NV, Ha do Q, Farrar JJ, Hien TT, Sedyaningsih ER, de Jong MD. 2010. A real-time RT-PCR for detection of clade 1 and 2 ...
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Original Article An allele specific locked nucleic acid real time quantitative PCR for detection of HBV rtA181V and rtN236T mutations
... specific locked nucleic acid quantitative PCR (RT-AS-LNA- qPCR) for early quantification of the rtA181V and rtN236T mutations associated with resistance to ADV and focused on its performance ...
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A real time RT PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes
... Results: We developed a single real-time RT-PCR assay for detection of both clades of H5N1 viruses, directly from clinical specimens, using locked nucleic acid TaqMan probes. Primers and probe used ...
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Therapeutic inhibition of microRNA-21 (miR-21) using locked-nucleic acid (LNA)-anti-miR and its effects on the biological behaviors of melanoma cancer cells in preclinical studies
... miRCURY LNA inhibitor ® (LNA-anti- miR-21), as well as its negative control, microRNA inhibitor negative control A (scrambled ...MiRCURY LNA inhibitor sequence is 5 ′ -3 ′ /56 FAM/ACA TCA GTC TGA TAA ...
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Structural transformation induced by locked nucleic acid or 2′–O-methyl nucleic acid site-specific modifications on thrombin binding aptamer
... which LNA and OMeNA are quite widely used in aptamer ...single LNA-substitutions for G2, T4, G5, T7, G8 or G15 have no effect on TBA structural alterations [19,20], and that LNA-substituted TBAs on ...
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Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L ) using in situ hybridization (ISH)
... A locked nucleic acid (LNA) type modified probe (LNA nucleotides are DNA nucleotide analogues) was chosen as they have shown to be superior to classical non-modified DNA probes in ...
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Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
... cleic acid greatly helped us study biological evolution, gene analysis, disease diagnosis, as well as drug dis- ...clear acid. Among these analogs, peptide nucleic acid (PNA) and locked ...
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Challenges to oligonucleotides-based therapeutics for Duchenne muscular dystrophy
... methoxyethyl (2 ’ O-MOE) and 2 ’ -O-aminopropyl, are RNaseH inactive and display higher nuclease resistance and affinity for target sequences than their 2 ’ -deoxy counterparts. Similar characteristics are found in ...
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Nucleic acid chaperons: a theory of an RNA-assisted protein folding
... to nucleic acids, there is no known specific amino acid complementarity, and the known physico- chemical rules (charge, hydrophobe, size compatibility) are often insufficient to define only one obvious ...
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Controls on development and nucleic acid indices in tropical planktonic copepods
... Table 1 — Relationship of nucleic acid indices with environmental variables, weight, growth and egg production in various planktonic organisms ........................................19 Table 2 — Regression ...
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Whole body composition of Xenopus laevis larvae: implications for lean body mass during development
... larval Ambystoma tigrinum, in which DNA content was linearly correlated with wet mass (A. W. Smits, unpublished data). Although few studies exist which have correlated nucleic acid content with wet or dry ...
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The relation between cell wall protein and nucleic acid synthesis in tall pea plants treated with a growth retardant AMO-1618.
... Cherry(l965). Homogenize the 2 gm of P -labeled tissue in a solution containing 40 ml 0.01 M Tris, pH 7.6, 0.06 M KCl, 0.01 M MgClg: 1 ml bentonite (40 mg/ml); 4.6 ml 11% Dupanol and 60 ml phenol with a Sorvall ...
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Advances in single molecule nucleic acid sequencing
... The sequencing chamber consists of a flexible plastic adhesive flow cell, less than a millimeter in height, which has been applied on the top of a low-autofluorescence microscope coverslip. The sequencing chamber holds ...
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Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus
... Nucleic acid detection kits for SFTSV (PCR-fluores- cence probe techniques) were obtained from SinoMD Gene (Beijing, China) and DaAn Gene (Guangzhou, ...
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