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MagNA Pure

Evaluation of the MagNA Pure LC Instrument for Extraction of Hepatitis C Virus RNA for the COBAS AMPLICOR Hepatitis C Virus Test, Version 2 0

Evaluation of the MagNA Pure LC Instrument for Extraction of Hepatitis C Virus RNA for the COBAS AMPLICOR Hepatitis C Virus Test, Version 2 0

... MagNA Pure LC sample preparation. (i) Standard-volume extraction. HCV RNA was extracted from 200-␮l aliquots of plasma or serum by utilizing the instrument’s Total Nucleic Acid External_Lysis protocol and ...

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Fully Automated, Internally Controlled Quantification of Hepatitis B Virus DNA by Real Time PCR by Use of the MagNA Pure LC and LightCycler Instruments

Fully Automated, Internally Controlled Quantification of Hepatitis B Virus DNA by Real Time PCR by Use of the MagNA Pure LC and LightCycler Instruments

... In this study we describe the development of a fully auto- mated real-time PCR (HBV LC-PCR) assay for the quantita- tive detection of HBV DNA in plasma with EDTA (EDTA plasma). The MagNA Pure LC instrument ...

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Use of the MagNA Pure LC Automated Nucleic Acid Extraction System followed by Real Time Reverse Transcription PCR for Ultrasensitive Quantitation of Hepatitis C Virus RNA

Use of the MagNA Pure LC Automated Nucleic Acid Extraction System followed by Real Time Reverse Transcription PCR for Ultrasensitive Quantitation of Hepatitis C Virus RNA

... the MagNA Pure or the manual QIAGEN ...the MagNA pure method (Fig. 2). Overall, the MagNA Pure LC extraction sys- tem showed very good linearity throughout the tested range (10 2 ...

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Reduced PCR Sensitivity Due to Impaired DNA Recovery with the MagNA Pure LC Total Nucleic Acid Isolation Kit

Reduced PCR Sensitivity Due to Impaired DNA Recovery with the MagNA Pure LC Total Nucleic Acid Isolation Kit

... The MagNA Pure LC total NA isolation kit (M extraction) on the MagNA Pure LC instrument provides generic extrac- tion chemistry on a medium- to high-throughput extraction ...

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Quantification of Hepatitis B Virus (HBV) DNA with a TaqMan HBV Analyte Specific Reagent following Sample Processing with the MagNA Pure LC Instrument

Quantification of Hepatitis B Virus (HBV) DNA with a TaqMan HBV Analyte Specific Reagent following Sample Processing with the MagNA Pure LC Instrument

... the MagNA Pure (MP) LC instru- ment (Roche Applied Science, Indianapolis, IN) used in con- junction with the MP total nucleic acid isolation kit (large volume; Roche Applied Science) have shown it to be a ...

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Assessment of MagNA Pure LC Extraction System for Detection of Human Papillomavirus (HPV) DNA in PreservCyt Samples by the Roche AMPLICOR and LINEAR ARRAY HPV Tests

Assessment of MagNA Pure LC Extraction System for Detection of Human Papillomavirus (HPV) DNA in PreservCyt Samples by the Roche AMPLICOR and LINEAR ARRAY HPV Tests

... the MagNA Pure LC, other automated nucleic acid purification platforms may pro- vide similar improvements for the processing of specimens prior to HPV ...

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Evaluation of the COBAS TaqMan HCV Test with Automated Sample Processing Using the MagNA Pure LC Instrument

Evaluation of the COBAS TaqMan HCV Test with Automated Sample Processing Using the MagNA Pure LC Instrument

... During the course of this study, we experienced no signifi- cant problems related to MP performance. The lower limit of detection and precision of TaqMan HCV with sample process- ing performed by MP were comparable to ...

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Automated extraction protocol for quantification of SARS Coronavirus RNA in serum: an evaluation study

Automated extraction protocol for quantification of SARS Coronavirus RNA in serum: an evaluation study

... the MagNA Pure LC instrument (Roche Diag- nostics, Basel, ...the MagNA Pure LC instrument extracts nucleic acids from biological specimens based on mag- netic bead ...

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Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition

Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition

... C16, MagNA Pure LC DNA isolation kit III, Easy-DNA gDNA purification kit, MP FastDNA Spin kit, PowerSoil DNA isolation kit, QIAamp DNA stool minikit, and QIAamp DNA stool minikit plus bead beating (for ...

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Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests

Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests

... automated Magna Pure NA ex- traction using a Magna Pure ...the Magna Pure RNA extraction system; however, RNA extraction using the QIAamp viral RNA extraction kit was very ...

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Multicenter Comparative Evaluation of Five Commercial Methods for Toxoplasma DNA Extraction from Amniotic Fluid

Multicenter Comparative Evaluation of Five Commercial Methods for Toxoplasma DNA Extraction from Amniotic Fluid

... methods, MagNA Pure Compact using nucleic acid isolation kit I and BioRobot EZ1 using the EZ1 DNA tissue kit, produced the best results, with a mean result of 60% positive reactions at the lowest ...

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Performance of Different Mono  and Multiplex Nucleic Acid Amplification Tests on a Multipathogen External Quality Assessment Panel

Performance of Different Mono and Multiplex Nucleic Acid Amplification Tests on a Multipathogen External Quality Assessment Panel

... The performances of the NAATs for the detection of the respiratory viruses subpanels 1 and 2 at laboratories 1 and 2 on extracts obtained with the routine MagNA pure LC and NucliSens Eas[r] ...

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Comparison of Commercial Extraction Systems and PCR Assays for Quantification of Epstein Barr Virus DNA Load in Whole Blood

Comparison of Commercial Extraction Systems and PCR Assays for Quantification of Epstein Barr Virus DNA Load in Whole Blood

... or MagNA Pure instrument; VL were quantified with the EBV R-gene quantifi- cation kit (Argene) or the artus EBV RG PCR kit (Qiagen) on the Rotor-Gene 6000 real-time analyzer; and the results were com- pared ...

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Benefits of haemoglobin in the cladoceran crustacean Daphnia magna

Benefits of haemoglobin in the cladoceran crustacean Daphnia magna

... It appears that our Hb-poor animals exhibited a higher tolerance for environmental oxygen deficiency which, however, can be attributed to the stringent selection of experimental animals and the specific experimental ...

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Magna Carta: Law, Liberty, Legacy

Magna Carta: Law, Liberty, Legacy

... It can then be hoped that this exhibition, and 2015’s commemorations more generally, may make modern "Mag-heads" of us all. This collaborative work is already helping us to understand afresh what happened around ...

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Directional Differences in the Colour Sensitivity of Daphnia Magna

Directional Differences in the Colour Sensitivity of Daphnia Magna

... The arrows labelled a show the direction of the infra-red observation light with respect to the experimental animal, while those labelled b give the path of the coloured stimulus light f[r] ...

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Multiple meanings in the Sanctuary of the Magna Mater at Ostia

Multiple meanings in the Sanctuary of the Magna Mater at Ostia

... the Magna Mater, and we may perhaps just hope that Abascantus may have derived some comfort from the rituals performed the next day when mourn- ing for Attis gave way to joy at his ...

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Transgenerational effects of microcystin LR on Daphnia magna

Transgenerational effects of microcystin LR on Daphnia magna

... With these results, we show transgenerational effects, which could improve the fitness of the population. Similar results have been reported for D. magna by Gustafsson and colleagues (Gustafsson and Hansson, 2004; ...

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Short communication-Survey of Methyl Tertiary Butyl Ether (MTBE) toxicity using bioassay on Daphnia magna

Short communication-Survey of Methyl Tertiary Butyl Ether (MTBE) toxicity using bioassay on Daphnia magna

... Since Methyl Tertiary Butyl Ether (MTBE) was used widespread as a fuel oxygenated in gasoline, it commonly contaminates ground water, drinking water, coastal waters, and other aquatic bodies (Werner et al., 2001). ...

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Magna Carta, the Rule of Law and the Limits on Government

Magna Carta, the Rule of Law and the Limits on Government

... But Bismarck could have saved his energy. Mirroring the declining fortunes of the rest of German classical liberalism after 1848 (when, as A.J.P. Taylor, 1945, famously said, “German history reached its turning-point and ...

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