Mammalian cell
Rescue and characterisation of Oropouche virus in mammalian cell lines
... viruses mammalian cell lines are transfected with three “transcription plasmids” that contain an antigenomic-sense cDNA copy of the three viral segments (Figure ...
316
Enhancement of product recovery in mammalian cell culture
... of mammalian cell culture are that the product is virtually always secreted, facilitating downstream processing and making cell immobilisation feasible, the proteins are folded correctly, and ...
223
Rapid high-throughput characterisation, classification and selection of recombinant mammalian cell line phenotypes using intact cell MALDI-ToF mass spectrometry fingerprinting and PLS-DA modelling
... of cell lines from the 96 DWP ...recombinant cell lines expressing different monoclonal antibodies and hence may be broadly applicable to the production of these high value ...production cell line ...
31
Control of recombinant protein productivity and quality in mammalian cell culture
... cumulative cell hours; CCH) specific production rates calculated (Table ...both cell lines. Although maximum cell numbers in the CHO cultures were lower than NSO cultures, TIMP-1 productivity was ...
234
Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell
... There are several physical methods for gene transfection. Gene gun or particle bombardment is one of them in which DNA will be shoot into the cells. In gene electroporation, another physical method, electrical pulses ...
32
Crude subcellular fractionation of cultured mammalian cell lines
... Akt, Cell Signaling Technology (CST #9272), GSK-3β, (CST #9315), Caspase-3 (CST #9662) and γ-Tubulin (Santa Cruz Biotechnology, sc-7396), Organellar; Calnexin (SIGMA #C4731), Calreticulin (Stressgen #SPA-061), ...
10
Viral cyclin disruption of mammalian cell cycle control mechanisms.
... U ntil now , precise identification of m am m alian CAK rem ained elusive. The cyclin H /c d k 7 complex can activate cdc2, cdk2 and cdk4 (Fisher and Morgan, 1994; Makela et al., 1994). There w as uncertainty as to w ...
192
The lactase enzyme: attempted induction in mammalian cell lines
... proved by intestinal biopsy that adult lactose malabsorption is caused by a deficiency of the enzyme, lactase, a specific intestinal beta-galactosidase in the small intestine where lacto[r] ...
122
Single mammalian cell gene expression analysis using microfluidics
... concentration of these enzymes to survive. Interestingly, when controlling for the basic cellular environment, by normalizing copy numbers of the glycolysis genes to β-actin gene expression, noise is seen to increase for ...
179
Propelling the paradigm shift from reductionism to systems nutrition
... vitro cell models. Cell lines of mammalian origin are now available from almost all tis- sues, but genotypic and phenotypic changes have been described to occur in most of them over time ...same ...
10
Green pyomelanin-mediated synthesis of gold nanoparticles: modelling and design, physico-chemical and biological characteristics
... two mammalian cell lines, namely mouse fibroblasts (NIH3T3) and human osteosarcoma cells ...(U2OS). Cell viability was only reduced at AuNPs concentration higher than 160 ...
11
Monitoring of fermentations with online capacitance sensor
... high cell density cultivations and especially for mammalian cell culture, for which the sensor was already successfully applied (Zhang et ...with cell-free media, fed the media with previously ...
5
"Clone": the history of a euphonious scientific term
... The application of the term clone by Sanford and colleagues to mammalian cell cultures originating from a single tissue cell-a technique first successfully performed in 1948-further exte[r] ...
22
Broad Tissue and Cell Tropism of Avian Bornavirus in Parrots with Proventricular Dilatation Disease
... Until the discovery of ABV, the Bornaviridae family con- sisted of a single genus, classical Borna disease virus (BDV). BDV is the causative agent of a progressive neurological disorder that primarily affects horses, ...
7
Vol 8, No 2 (2019)
... A series of Rhodanine derivatives were synthesized by Knoevenagel condensation. All the synthesized compounds were tested for their in vitro anticancer activity against MCF-7 and BT-474 human breast cancer cell ...
8
Emerging role of Hpo signaling and YAP in hepatocellular carcinoma
... Consonant with the fundamental roles of Hpo signaling, alterations of Mst1/2, Sav, Lats1/2, and Nf2/mer lead to excess proliferation and – in some cases – carcinogenesis in the mammalian liver. Across the globe, ...
10
Toxicity of Nanoparticles and an Overview of Current Experimental Models
... in cell organelles (endosomes/lysosomes), release into cytoplasm after decomposing, and contribut to cellular iron ...of cell lysis, inflammation, and disturbing blood coagulation system [47] ...reduced ...
11
RNF17, a component of the mammalian germ cell nuage, is essential for spermiogenesis
... dramatic cell proliferation and differentiation (Russell et ...stem cell spermatogonia undergo self-renewal, proliferation and ...meiotic cell divisions result in haploid ...meiotic cell ...
11
Basis of PD1 PD-L1 Therapies
... B cell or T cell antigen receptor thereby yielding co-inhibitory micro-clusters with the T cell receptor (TCR) and ...different cell types contributes to distinct ...e.g., cell cycle ...
14
Cell migration, intercalation and growth regulate mammalian cochlear extension
... of cell number within a section of the epithelium extending along 100 µm of the axis of extension [base-to-apex (B-A)] indicates a significant decrease of ∼ 42% between E14 and E16 ...in cell volume on ...
11