MCF-7 cell culture
Original Article Regulatory role and mechanism of NAC1 in cancer cell survival subjected to hypoxia
... tumor cell culture medium between NAC1 expression group and NAC1 silence ...of MCF-7 cell culture under hypoxic conditions (1% O 2 ), in comparison with the control group, the ...
7
Original Article Sensitization to tamoxifen by tanshinone IIA in tamoxifen-resistant breast cancer cells in vitro
... Total miRNA-enriched RNAs from cell culture were extracted from MCF-7 and MCF-7-TamR cells following 0.05 µM of TSA treatment for 5 days using TRI Reagent (Sigma, St Louis, MO, ...
12
Gene expression profiling identifies activated growth factor signaling in poor prognosis (Luminal-B) estrogen receptor positive breast cancer
... assays: MCF-7 cells cultured at low density in standard 60 mm tissue culture ...assays: MCF-7 cells in ...starved MCF-7 cells were treated with vehicle (control), ...
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AKR1B10 promotes breast cancer metastasis through integrin α5/δ-catenin mediated FAK/Src/Rac1 signaling pathway
... cells MCF-7 and MDA-MB-231 or siRNA-mediated silencing in BT-20 cells affected cell adhesion, migration and invasion in cell culture, and metastasis to the lung in the nude mice through ...
13
Adipocyte lipolysis links obesity to breast cancer growth: adipocyte-derived fatty acids drive breast cancer cell proliferation and migration
... increase MCF-7 prolifer- ation compared to lean donors ...to MCF-7 cells was also observed under obese conditions, but this was not associated with a further increase in mitochondrial fatty ...
14
<p>Long Non-Coding RNA UCA1 Modulates Paclitaxel Resistance in Breast Cancer via miR-613/CDK12 Axis</p>
... All cell lines used in the current study were purchased from the American Type Culture Collection (ATCC, Manassas, VA, ...BC cell line MCF-7 was maintained in Dulbecco ’ s Modi fi ed ...
12
Phenol red in the culture medium strongly affects the susceptibility of human MCF-7 cells to roscovitine
... Endocrine-disrupting compounds are of particular concern as they not only have the capacity to interfere with the natural production and metabolism of hormones in the body, but also represent a serious carcinogenic risk. ...
14
Original Article Triptolide reverses MCF-7/ADR cell resistance by down-regulating P-glycoprotein expression
... then incubated for 24 h. Fresh media contain- ing different concentrations of adriamycin (10- 200 μg/ml) and triptolide (10-320 nmol/L) as well as a combination of adriamycin and trip- tolide were added separately, after ...
9
Chitosan–alginate BSA-gel-capsules for local chemotherapy against drug-resistant breast cancer
... purchased MCF-7 cell line from the Chinese Academy of Sciences Cell Bank of Type Culture Collection (Shanghai, ...China). MCF-7 cells were cultured in Roswell Park ...
14
Evaluation of Human Breast Adenocarcinoma (MCF-7) Cells Proliferation in Co-Culture with Human Adipocytes in Three Dimensional Collagen Gel Matrix: Norepinephrine as a Lipolytic Factor
... Cell line and preparation of adipocytes. As typical breast carcinoma cells, we used MCF-7 (NCBI C135) breast cancer cell line (obtained from National Cell Bank of Iran, Tehran). Human ...
7
Antrodia cinnamomea extract inhibits the proliferation of tamoxifen resistant breast cancer cells through apoptosis and skp2/microRNAs pathway
... carcinoma cell line ...tamoxifen-resistant MCF-7 and MCF-7 cells were ...different culture duration and different breast cancer cell ...
11
Catalase down-regulation in cancer cells exposed to arsenic trioxide is involved in their increased sensitivity to a pro-oxidant treatment
... of MCF‑7 cell line stably expressing catalase Breast cancer cell line MCF-7 CAT 3 overexpressing catalase was established from wild-type MCF-7 cell line, ...
9
Anticancerous activity of anthocyanin analyzed using different cell lines from the onion peel (allium cepa) extraction
... 96-well culture plates in different concentrations of red onion and big onion peel extracts of Allium cepa to determine their anticancer effects using the MTT ...against MCF-7 cell ...
5
Membrane estrogen receptor α levels in MCF 7 breast cancer cells predict cAMP and proliferation responses
... different culture conditions, assay incubation conditions, and specific response mimetics or inhibitors allowed us to implicate pathways and signal levels in the mechanism of control of cell number, and to ...
12
Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro
... The R. rosea extract and salidroside inhibit transla- tion initiation. Both the R. rosea extract and salidroside treatment of UMUC3 bladder cells caused a significant percentage of cells undergoing autophagy. The R. ...
9
Original Article ClC5 is upregulated in paclitaxel resistant breast cancer cells and decreases paclitaxel sensitivity via promoting HMGB1 translocation
... eration, cell death resistance and ...cancer cell line MCF-7 cells. We firstly established in vitro MCF-7 cells and paclitaxel- resistant MCF-7/PTX cells as ...
10
Combined Androgen receptor blockade overcomes the resistance of breast cancer cells to palbociclib
... of cell cycle, AR short hairpin RNA (shAR) was transfected into the MCF-7pR ...from cell cycle analysis showed that DHT treatment resulted in a decrease of cells in G0/G1 phase and a concurrent ...
11
Original Article Overexpression of long non-coding RNA HOTAIR enhances breast cancer radioresistance via RhoC-Akt pathway by targeting HOXD10
... into cell line SKBR3 using liposome, thus overexpressing HOTAIR gene in the ...each cell lines were assessed following overexpressing HOTAIR gene by cell proliferation/viability tests by means of ...
9
Stimuli-responsive nanodrug self-assembled from amphiphilic drug-inhibitor conjugate for overcoming multidrug resistance in cancer treatment
... the MCF-7 cells and MCF-7/ADR cells were incubated with Ir-ss-Qu NPs, the concentration of Ir was still kept at a high level in both MCF-7 cells and MCF-7/ADR cells ...
14
NANOEMULSION BASED CAMPHOR OIL CARRYING IFOSFAMIDE: PREPARATION, CHARACTERIZATION, AND IN VITRO EVALUATION IN CANCER CELLS
... Moreover, previous studies showed that encapsulation of essential oils into emulsion due to the hydrophobic nature of oil leads to higher solubility and dispersibility in aqueous media besides the reduction of the ...
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