mean fluorescence
Using the geometric mean fluorescence intensity index method to measure ZAP-70 expression in patients with chronic lymphocytic leukemia
... geometric mean fluorescence intensity (geo MFI) index method: this approach was based on the evaluation of ZAP-70 expression levels in terms of geo MFI ...geo mean of CLL cells was 10 ± 1 (tube ...
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Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System
... the mean fluorescence of cells measured for C-GON uptake was slightly decreased in the presence of shear stress (Figure ...by fluorescence microscopy (Figure ...Green fluorescence imaging was ...
13
Cytokines Elicited by HSP60 in Periodontitis with and without Coronary Heart Disease
... low mean fluorescence intensity (Table ...the mean fluores- cence intensity of TH1 cytokines in both controls and patients with CP and CHD (Table ...creased mean fluorescence intensity ...
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Changing Viral Tropism Using Immunoliposomes Alters the Stability of Gene Expression: Implications for Viral Vector Design
... the mean fluorescence intensity of cell ...the mean (% transfection efficiency) ± SD of triplicates (A) and the mean fluorescence intensity ...
11
Chagasic patients are able to respond against a viral antigen from influenza virus
... percentage, mean fluorescence intensity (MFI) and integrated mean fluorescence intensity (iMFI) obtained multiplying the frequency by the ...
8
Measles Virus Spread by Cell-Cell Contacts: Uncoupling of Contact-Mediated Receptor (CD46) Downregulation from Virus Uptake
... The activity of FIP was controlled by analysis of its capacity to inhibit the infection of HeLa cells with MV-Edm (MOI 5 1) for 48 h. In the presence of 200 mM FIP the infection was completely blocked, whereas in the ...
11
Assessing the Efficacy of Nano- and Micro-Sized Magnetic Particles as Contrast Agents for MRI Cell Tracking
... the mean green fluorescence of individual ...the mean fluorescence intensity as a function of contrast agent concentration was exactly the same as observed quantitatively by intracellular iron ...
12
Association between autophagy and inflammation in patients with rheumatoid arthritis receiving biologic therapy
... Fig. 2 Representative cytometric histograms of p62 levels in lymphocytes (a1 and b1), monocytes (a2 and b2), and granulocytes (a3 and b3) from one patient with rheumatoid arthritis (RA) and one healthy control subject ...
11
YC-1 induces lipid droplet formation in RAW 264.7 macrophages
... hydrophobic fluorescence dye to detect intracellular lipid droplets and ox-LDL-induced lipid accumulation in macrophages has been reported in the research literature ...the mean fluorescence ...
9
The expression of the chemokine receptor CCR5 in tick-borne encephalitis
... Fig. 3 CCR5 expression in TBEV-infected patients compared to other patient groups and healthy controls. Expression of CCR5 in the activated Th lymphocyte population (CD3+CD4+CD45RO) measured cytometrically as described ...
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The importance of claudin-7 palmitoylation on membrane subdomain localization and metastasis-promoting activities
... h; mean number ± SD (triplicates) of invading and penetrating cells and representative examples (scale bar: 250 μ m); significant differences between HEK-EpC-cld7 and HEK-EpC mAG -cld7 or HEK-EpC-cld7 mPalm : ...
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Rapid susceptibility testing of Candida albicans by flow cytometry
... inoculated with each dye at ambient temperature for approximately 5 min before being analyzed with a Bryte HS flow cytometer (Bio-Rad, Hercules, Calif.) with WinBryte software or a FACScan flow cytometer (Becton ...
6
Treatment of systemic lupus erythematosus patients with the BAFF antagonist “peptibody” blisibimod (AMG 623/A 623): results from randomized, double blind phase 1a and phase 1b trials
... A limitation of these studies is that this population had mild stable or inactive disease (mean SLE Disease Activity Index score of approximately 3) and baseline immune-phenotypic profiles that were more similar ...
14
Autonomous buckling of micrometer-sized lipid-protein membrane patches constructed by Dictyostelium discoideum
... film of Dictyostelium prepared by the spin coating method on the millimeter scale by light microscopy. We found a lipid membrane film pattern on the cover glass (Figure 2A), which was held by the aspiration pores of the ...
11
Nano-sized cytochrome P450 3A4 inhibitors to block hepatic metabolism of docetaxel
... the fluorescence seen in the liver did not hide other potential sites of accumulation, the concentrations of PLGA, PLGA-Ga and HA nanoparticles were lowered from 4 g/L to ...The mean ratio ...
20
Fluorescence as a Tool to Study Lipid Protein Interactions: The Case of α Synuclein
... the early 2000s [22,23]. While the AFM images obtained by this group suggest pore-like mechanism, quantitative biophysical information on the structures and composi- tion of these oligomeric aggregation intermediates is ...
8
An Immunogenomics Approach to Understanding Periparturient Immunosuppression and Mastitis Susceptibility in Dairy Cows*
... The wide distribution of the clustered Cy5 spots in the scatter plot in Fig. 4 indicated wide variation in red dye intensities amongst these 18 BOTL cDNAs. These could be seen on the actual microarray as spots that ...
18
Leukocyte, Haemostatic and Inflammatory Biomarkers Are Activated in Patients with Cardiovascular Disease (CVD): A Pilot Study
... 21 patients were recruited at random from the CVD clinic at Russells Hall Hospital, and all had CVD due to atherosclerosis (n=21). Specifically, the CVD group consisted of seven females and 14 males, aged 36-79 years ...
15
Study of Myeloid Antigens CD13 and CD33 Expression in Sudanese Patients with Acute Leukemia
... Regarding the flowcytometry parameters, we found that CD13 percentage level and mean intensity showed a significant differentiation between sub group of AML (p.value= 0.05 & 0.038, respectively) while they ...
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Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting
... using fluorescence minus one (FMO) controls [27], which con- tained cells stained with all fluorophores except the one conjugated to the surface marker of ...
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