Melting curve analysis for all samples
Microcalorimetric Biosensors for DNA Melting Curve Analysis.
... dry samples exposed to ambient ...liquid samples: evaporation and associ- ated changes in biomolecule concentration and sample ...liquid samples the polydimethylsiloxane (PDMS) reaction chamber ...
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Rapid Identification of Clinically Relevant Mycobacterium Species by Multicolor Melting Curve Analysis
... In this study, we evaluated the analytical and clinical performances of the MeltPro Myco assay. The MeltPro Myco assay accurately identified 51 reference mycobacterial strains to the species/genus level and showed no ...
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Identification and species-typing of wood rotting fungi using melting curve analysis
... DNA sequencing has been the main method used to identify microbial species including fungi, but the process requires an enormous amount of time, cost, and effort in some cases, such as for the stock management of ...
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Detection and Differentiation of In Vitro Spiked Bacteria by Real Time PCR and Melting Curve Analysis
... clinical samples or in case of the detection of “Staphylococcus species,” another PCR run of about 30 min with specific primers and probes should be done to be on the safe ...
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McSpoligotyping, a One Step Melting Curve Analysis Based Protocol for Spoligotyping of Mycobacterium tuberculosis
... DISCUSSION In this study, we described for the first time a single-step spoligotyping protocol based on melting curve analysis. As no post-PCR manipulations were required, the protocol was simple and ...
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Detection and Genotyping of Oocysts of Cryptosporidium parvum by Real Time PCR and Melting Curve Analysis
... I melting curve analysis was used to confirm the specificity of the method when DNA extracted from fecal samples spiked with oocysts was ...of melting curve ...of melting ...
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Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR
... the analysis of inhomogeneously methylated DNA samples ...the melting curve peak position shifts to a higher temper- ature the more CpGs are methylated within a given ...among samples ...
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Simultaneous Detection, Genotyping, and Quantification of Human Papillomaviruses by Multicolor Real Time PCR and Melting Curve Analysis
... of samples, the throughput would be substantially decreased, while the labor and the overall cost would be increased ...30 samples could be tested per run in a standard 96-well real-time thermocycler, and ...
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Detection of Anti Hepatitis B Virus Drug Resistance Mutations Based on Multicolor Melting Curve Analysis
... serum samples ob- tained from different patient populations demonstrated the accu- FIG 3 Comparison of PCR Sanger sequencing, COLD-PCR Sanger sequencing, and the MMCA method for minority rtM204V mutation ...
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<p>Analysis of the reannealing- instead of melting-curve in the detection of JAK2 V617F mutation by HRM method</p>
... the analysis of HRM is essential for ...and melting-curve analysis methods follow- ing the HRM analysis with the aim to introduce the more ef fi cient one for JAK2 V617F mutation ...
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Analysis of multiple single nucleotide polymorphisms closely positioned in the ovine PRNPgene using linear fluorescent probes and melting curve analysis
... imbalanced melting peaks were reliably detected. The bottle-neck for analysis within our laboratory was the number of PCR plates that could be prepared per ...of samples, without inclusion of probes ...
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Rapid Detection of Isoniazid Resistance in Mycobacterium tuberculosis Isolates by Use of Real Time PCR Based Melting Curve Analysis
... The clinical validation study, which enrolled 1,096 clinical iso- lates that included 437 INH-resistant and 659 INH-susceptible isolates, represented one of the largest-scale validation studies in terms of sample number. ...
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Detection of monoclonal immunoglobulin heavy chain gene rearrangement (FR3) in Thai malignant lymphoma by High Resolution Melting curve analysis
... Resolution Melting curve analysis (HRM) The PCR product of IgH variable region 3 was further amplified in the LightCycler System 480 (Roche Diagnos- ...resolution melting master 10 μl, 5 ...
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Rapid detection of immunoglobulin heavy chain gene rearrangement by PCR and melting curve analysis using combined FR2 and FR3 primers
... by melting curve analysis in LightCycler system, which is based on a fundamental DNA ...96 samples simultaneously within 90 ...DNA melting curve analysis in a closed ...
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Simultaneous Quantification and Genotyping of Hepatitis B Virus for Genotypes A to G by Real Time PCR and Two Step Melting Curve Analysis
... Moreover, the method was found to have higher precision in genotyping and sensitivity for mixed-genotype detection equal to or higher than that of RFLP. A limitation of the method is that the genotype is not identified ...
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Detecting differential allelic expression using high-resolution melting curve analysis: application to the breast cancer susceptibility gene CHEK2
... HRM analysis include its rapidity, cost-effectiveness and secur- ity due to its closed-tube ...of samples to test, analysis with the HR-1 ™ instrument ended up being much more time consuming (Table ...
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A novel protocol to isolate, detect and differentiate taeniid eggs in leafy greens and berries using real time PCR with melting curve analysis
... not all taeniids, the Eva- Green system could potentially be replaced by specific primer-probe pairs to enhance ...field samples to determine diagnostic sensitivity and specific- ity before it could be ...
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Ultrasensitive Detection of Drug Resistant Pandemic 2009 (H1N1) Influenza A Virus by Rare Variant Sensitive High Resolution Melting Curve Analysis
... patient samples, standard HRM analysis was used as a foundation to create ...the melting curves of the resulting amplifica- tion products from each well were subjected to HRM ...
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Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping
... a melting curve analysis genotyping method based on real-time PCR using hybridization probes to detect the M815I-T917I-L920F knockdown resistance (kdr) mutation in the paraorthologous ...
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Simple Curve Samples
... California Department of Transportation Introduction Route surveying is comprised of all survey operations required for design and construction of engineering works such as highways, pipelines, canals, or ...
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