Merozoite Surface Protein 10
Genetic diversity of Plasmodium vivax isolates from Azerbaijan
... The merozoite surface protein 3α (MSP3-α) gene also seems to be a good candidate for studying the genetic diversity of ...circumsporozoite protein (CSP) and merozoite sur- face ...
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Genetic polymorphism of merozoite surface protein 2 and prevalence of K76T pfcrt mutation in Plasmodium falciparum field isolates from Congolese children with asymptomatic infections
... Genotyping of merozoite surface protein-2 (msp2) is the commonly used method to study the genetic diversity of P. falciparum. This gene presents polymorphisms in the number of repeats and sequence ...
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Asymptomatic Plasmodium falciparum infection in children is associated with increased auto antibody production, high IL 10 plasma levels and antibodies to merozoite surface protein 3
... on 10% SDS-PAGE at 25 mA, and were transferred onto nitrocellulose mem- branes (Schleicher & Schull, Dassel, Germany) by semi-dry electrotransfer (Pasteur Institute, Paris) for 1 h at ...
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Genetic polymorphism of merozoite surface protein 1 and merozoite surface protein 2 in Plasmodium falciparum field isolates from Myanmar
... The oligonucleotide primers specific for the polymorphic regions (block 2 of MSP-1 and block 3 of MSP-2) were designed as described previously [17]. The two genes were amplified by nested PCR. An initial amplification of ...
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Characterization of Pv92, a novel merozoite surface protein of Plasmodium vivax
... The amine-coated slides were prepared as described previ- ously [4]. A total of 32 or 20 serum samples from vivax pa- tients or healthy persons, respectively, were used to evaluate the antigenicity of Pv92. One-hundred ...
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Genetic diversity of the merozoite surface protein 3 gene in Plasmodium falciparum populations in Thailand
... MSP-3 protein has a crucial role not only in binding to the host red blood cell, but also in protecting the parasite against haem that is released during parasite egression ...MSP-3 protein is synthesized ...
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Plasmodium falciparum merozoite surface protein 1: Antigenicity, immunogenicity and structure.
... to 10 days depending on the Plasmodium species (5-6 days for ...the merozoite surface and the erythrocyte ...The merozoite then reorients so that its apical end, which contains organelles ...
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Identification and characterization of Pv50, a novel Plasmodium vivax merozoite surface protein
... conserved protein, Pv50 (PVX_087140) was identified in ...hypothetical protein Pv50, its immunogenic profiles, humoral and cellular immune responses, pro- tein interactions and subcellular localization in ...
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IMMUNOGENICITY OF THE MEROZOITE SURFACE PROTEIN-1 (MSP-1) OF HUMAN PLASMODIUM SP.
... Besides AS02A, MSP-1 42 was tested with a few other different adjuvants. The adjuvant Alhydrogel was formulated to Plasmodium falciparum MSP-1 42 , with both 3D7 and FVO alleles, and evaluated in 60 volunteers in USA. ...
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Polymorphic patterns of the merozoite surface protein 3β in Korean isolates of Plasmodium vivax
... Genomic DNA extraction and amplification of PvMSP-3 β Genomic DNA was extracted from 200 μl of whole blood sample using the QIAamp DNA Blood Kit (Qiagen, Hilden, Germany). The full-length region encoding Pv MSP-3β was ...
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Results from tandem Phase 1 studies evaluating the safety, reactogenicity and immunogenicity of the vaccine candidate antigen Plasmodium falciparum FVO merozoite surface protein 1 (MSP142) administered intramuscularly with adjuvant system AS01
... 10 μg group began in April 2008 with vaccinations for the 50 μg group starting three weeks later after safety review. One subject in the low-dose group was withdrawn after the first vaccination due to a prolonged ...
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Multiplicity and molecular epidemiology of Plasmodium vivax and Plasmodium falciparum infections in East Africa
... and merozoite surface protein (msp) genotyping [14–17], for assessing MOI estimation can lack both sen- sitivity and specificity, resulting in the apparent problem of underestimating disease ...
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Diversity and evolutionary genetics of the three major Plasmodium vivax merozoite genes participating in reticulocyte invasion in southern Mexico
... Plasmodium merozoite invades reticulocytes in a few seconds by a series of complex ...ozoite surface protein 1 (MSP1) participates in the initial adhesion to the reticulocyte and the apical membrane ...
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Merozoite surface protein-7. A novel protein on the surface of Plasmodium falciparum merozoites
... After an additional 2 weeks growth to increase the antibody titre in the culture supernatant, the primary hybridoma colony supernatants were re-screened by IFAT (Figure 4.8). Supernatant from five wells o f the primary ...
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Immunogenicity of a chimeric Plasmodium falciparum merozoite surface protein vaccine in Aotus monkeys
... There is mounting agreement in the field that an effec- tive malaria vaccine will require induction of immune responses to multiple, distinct target antigens. This con- cept is central in the development of whole ...
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A Novel Malaria Pf/Pv Ab Rapid Diagnostic Test Using a Differential Diagnostic Marker Identified by Network Biology
... Rapid diagnostic tests (RDTs) offer accurate and timely diagnosis of malaria in endemic areas and are easily accessible, cost effective, and give timely results [2]. Currently, more than 200 malaria RDTs, especially ...
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Antibody levels against GLURP R2, MSP1 block 2 hybrid and AS202 11 and the risk of malaria in children living in hyperendemic (Burkina Faso) and hypo endemic (Ghana) areas
... parasite surface proteins have been associated with reduced risk of malaria ...ite surface protein (MSP)1 is synthesized in a precursor form as a 195kD protein and proteolytically cleaved into ...
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Antibody responses to P falciparum blood stage antigens and incidence of clinical malaria in children living in endemic area in Burkina Faso
... MSP3 and GLURP have been tested in malaria vaccine antigens that have already undergone phase 1 trials. The efficacies of these malaria vaccines will be tested in phase II and III vaccine trials in Africa. For the ...
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Plasmodium falciparum merozoite surface protein 2: epitope mapping and fine specificity of human antibody response against non polymorphic domains
... is merozoite surface protein 2 (MSP2), a blood-stage protein that appears to be essential for viability and completion of the Plasmodium life cycle in humans ...
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Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
... Background: Babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in Europe and west Asia. Despite its importance, there is no known rapid diagnostic kit detection of ...
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