Metabolic Engineering
Metabolic engineering of roseoflavin-overproducing microorganisms
... by metabolic engineering over the last decades [30, 38] and appeared to be a good host for roseoflavin production for two reasons: First, riboflavin overproduction protects from the toxic effect of ...
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Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene
... of metabolic engineering, many research labs have explored biosynthetic methods of monoterpene production by introducing heterologous monoterpene synthase: for in- stance, 3-carene and ...
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Leveraging Extreme Thermoacidophily for Archaeal Metabolic Engineering.
... thermophilic metabolic engineering host is facilitated purification of volatile chemicals from the vapor phase of an actively growing ...for metabolic engineering of an extreme thermophile for ...
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Metabolic Engineering of Microorganisms for the Production of Higher Alcohols
... FIG 1 Strategies for the production of linear, primary alcohols. (A) Production of 1-butanol and 1-hexanol through the native or reconstructed clostridial pathway. The dotted arrow adjacent to Bcd-EtfAB indicates the ...
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A toolset of constitutive promoters for metabolic engineering of Rhodosporidium toruloides
... To improve prospects for more advanced metabolic engineering in this fungal host, the development of robust genetic tools and resources is an immediate pri- ority [8]. A major need on the tools front is for ...
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Challenges and tackles in metabolic engineering for microbial production of carotenoids
... pathways. Engineering strategies include enhancement of IPP and DMAPP precursors supply by overexpression of rate- limiting enzyme of the MEP or MVA pathway, optimi- zation of heterologous crt pathway, and ...
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Metabolic engineering of microbial cell factories for production of nutraceuticals
... Metabolic engineering of nutraceutical products pro- vides an attractive alternative to chemical synthesis and extraction that enables enantiomerically pure com- pounds to be produced at benign conditions ...
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Overview of Multiobjective Optimization Methods in in Silico Metabolic Engineering
... silico Metabolic Engineering, the identification of reaction knockout strategies that produce mutant strains with a permissible growth rate and product rate of desired metabolites is still ...silico ...
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Metabolic engineering of microbes for oligosaccharide and polysaccharide synthesis
... good metabolic engineering targets for rational engineering of polymer properties, provided that the gene-to-enzyme activity rela- tionships are understood in sufficient ...
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An ancient Chinese wisdom for metabolic engineering: Yin-Yang
... In ancient Chinese philosophy, Yin-Yang describes two contrary forces that are interconnected and interdependent. This concept also holds true in microbial cell factories, where Yin represents energy metabolism in the ...
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Application of metabolic engineering approaches in enhancing biological hydrogen production
... central metabolic precursor for the bioproduction of ...for metabolic engineering include the deletion of lactate dehydrogenase (ldh) and fumarate reductase (frdBC) as well as the overexpression of ...
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High production of valencene in Saccharomyces cerevisiae through metabolic engineering
... The course profiles in bioreactor (Fig. 7b) showed that glucose was almost entirely consumed and the biomass increased by twofold compared to shake flask. How- ever, the cell density was still relatively low in fed-batch ...
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Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein
... apo-PBP. In this study, the biosynthesis pathway for phycobiliprotein was constructed using one expression plasmid, which had significantly increased plasmid sta- bility. Moreover, strategies for the directed evolution ...
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Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor
... [8]. The methylotrophic metabolism in M. extorquens AM1 involves three interlocked cycles: the serine cycle, the ethylmalonyl-CoA pathway (EMC pathway) and the poly-3-hydroxybutyrate (PHB) cycle (Fig. 1) [9]. Notably, ...
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Metabolic engineering of microorganisms for production of aromatic compounds
... conventional metabolic engineering strategies such as the elimination of competing pathways through inactivation of enzymes involved in the central carbon metabolism ...
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Development of Computational Tools for Metabolic Engineering
... In metabolic engineering, major components of metabolism could be completely redesigned for more efficient utilization of resource pools to minimize material ...algorithms, metabolic network analysis ...
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Metabolic engineering of a toluene degradation pathway
... of metabolic engineering, extending a host pathway, involves the post-translational modification of a naturally expressed product by means of a heterologous protein processing ...
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Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway
... synthetic metabolic engineering and demonstrated its application to the construction of the non-ATP-forming chimeric EM ...Synthetic metabolic engineering enables a one-step preparation of ...
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Metabolic engineering strategies for the improvement of cellulase production by Hypocrea jecorina
... In addition to cellulose-containing plant polysaccharide mixtures, D-galactosyl-β-1,4-D-glucoside lactose is able to induce cellulase gene expression. The obvious advantage of lactose over cellulose is that it is soluble ...
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Metabolic engineering of Corynebacterium glutamicum for anthocyanin production
... Background: Anthocyanins such as cyanidin 3-O-glucoside (C3G) have wide applications in industry as food color- ants. Their current production heavily relies on extraction from plant tissues. Development of a sustainable ...
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