Metabolic Flux
Metabolic flux and transcriptional analysis elucidate higher butanol/acetone ratio feature in ABE extractive fermentation by Clostridium acetobutylicum using cassava substrate
... red. Metabolic flux analysis indicated that the rates of or- ganic acid formation and re-assimilation were promoted after neutral red addition, although the concentrations of acids in broth had not an ...
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Metabolic-Flux Profiling of the Yeasts Saccharomyces cerevisiae and Pichia stipitis
... using metabolic-flux ratio analysis by nuclear magnetic reso- ...active metabolic pathways was compared to those identified in Saccharomyces cerevisiae, which led to the following ...
11
A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis
... Results: The development of a chemically defined growth medium allowed rapid growth of P. sapidus in submerged cultures. As P. sapidus grew extremely slow in salt medium, the co-utilization of amino acids using 13 ...
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Metabolic engineering of Synechococcus elongatus PCC 7942 for improvement of 1,3-propanediol and glycerol production based on in silico simulation of metabolic flux distribution
... of metabolic flux distribution were obtained from 36 strains in the fourth ...same metabolic flux distribution were observed in different two-gene disruptions (Synpcc7942_0191 and 1501, and ...
12
Quantification of folate metabolism using transient metabolic flux analysis
... All metabolic flux analysis methodologies, including our tMFA implementation, are limited to the biochemical pathways build in into the ...this flux is beyond the focus of this work on folate ...
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Studying metabolic flux adaptations in cancer through integrated experimental-computational approaches
... metabolic flux. This can be partially overcome by considering a metabolic network model in which metabolite pools and reactions are localized in dif- ferent compartments, and inferring the isotope ...
11
13C-metabolic flux analysis of ethanol-assimilating Saccharomyces cerevisiae for S-adenosyl-l-methionine production
... the flux of cytosolic oxaloacetate to SAM biosynthesis corre- sponded to ...the metabolic flux data were compared with the NADPH consumption rates for cell ...
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ENZYME VARIATION, METABOLIC FLUX AND FITNESS: ALCOHOL DEHYDROGENASE IN DROSOPHILA MELANOGASTER
... We report here such measurements of the metabolic flux in the conversion of ethanol to the two products, CO2 and lipids, for six different active genotypes, containi[r] ...
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Metabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains
... the metabolic fluxes with 13 C-labeled glucose in two genetically modified, SA-producing strains of ...significant metabolic flux changes occurred for the shikimate pathway, tricarboxylic acid (TCA) ...
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Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida
... the metabolic flux through this step by overproducing the rate-limiting, succeeding enzyme (Pgl) failed to increase PHA production (Figure ...the flux of carbon towards PHA was most likely hampered ...
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Assessing and Resolving Model Misspecifications in Metabolic Flux Analysis
... the metabolic network. The accuracy of the flux estimates is often a concern in the application of the overdetermined ...intracellular metabolic fluxes, have been formulated ...the flux ...
15
Metabolic flux control of mitochondrial dynamics in cancer cells
... enrichment of HKII to the MOM alone is insufficient to prevent cell death. HKII bound to the mitochondria may be protective against apoptosis but only in the presence of glucose. An increase in HKII expression in ...
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Metabolic Flux and Fitness
... First, the control coeffi- cient of the P-galactosidase enzyme with regard to fitness is quite small ( C = 0.018); therefore, genetic variants demonstrating small[r] ...
7
OpenFLUX: efficient modelling software for 13C-based metabolic flux analysis
... Not all intracellular fluxes can be resolved in a single labelling experiment, but careful experimental planning can ensure that key unknown fluxes are determinable [34,35]. Determining the optimal labelled substrates to ...
15
13C metabolic flux analysis-guided metabolic engineering of Escherichia coli for improved acetol production from glycerol
... together with analysis of redox states revealed that NADPH regeneration is a limiting step, and the NADPH availability and well redox balance status are crucial for efficient acetol biosynthesis. We tested our hypothesis ...
13
Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger
... the flux through TCA cycle pathway was ...the flux directed into the TCA cycle ...the flux of the TCA cycle pathway dropped. Similarly, we found that this flux changed in the same direction in ...
14
Tissue-specific metabolic reprogramming drives nutrient flux in diabetic complications
... of metabolic enzymes by acetyl- ...specific metabolic enzymes were likely functional- ly important and not the result of a global response to increased ...by metabolic flux studies (Figure 4) ...
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Ethylene-forming enzyme and bioethylene production
... pathways, metabolic bottlenecks and competing pathways can be revealed by systems biology approaches such as flux balance analysis (FBA) and metabolic flux analysis ...control flux ...
11
A kinetic metabolic study of lipid production in Chlorella protothecoides under heterotrophic condition
... key metabolic processes during lipid synthesis thus refining culture/genetic strategies to maximize cell lipid ...kinetic metabolic model simulating cell meta‑ bolic behavior and lipid production was first ...
13
Integrating kinetic model of E coli with genome scale metabolic fluxes overcomes its open system problem and reveals bistability in central metabolism
... alternative metabolic state predicted by the kinetic model possesses an increased concentration in fdp and a decreased concentration in ...reinforced flux in glycolysis, as induced from the inhibition of ...
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