Metabolic Flux Analysis
A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis
... Results: The development of a chemically defined growth medium allowed rapid growth of P. sapidus in submerged cultures. As P. sapidus grew extremely slow in salt medium, the co-utilization of amino acids using 13 ...
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13C metabolic flux analysis on roles of malate transporter in lipid accumulation of Mucor circinelloides
... carbon flux of the control strain through the EMP and PP pathway accounted for ...the flux ratios of EMP/PP pathway in mt-overexpression strains were decreased to ...higher flux of PP path- way in ...
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13C-Metabolic Flux Analysis in Developing Flax Linum usitatissinum L.) Embryos to Understand Storage Lipid Biosynthesis
... comprehensive analysis of metabolic flux in flax is still ...glutamine. Metabolic fluxes were estimated by computer-aided modeling of the central metabolic network including 11 ...
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Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells
... a metabolic steady state was attained; the consumption and production rates of key metabolites stabilized after a key length of ...glycolytic flux caused a reduction in the sialyla- tion and antennarity of ...
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Quantification of folate metabolism using transient metabolic flux analysis
... [14], metabolic flux analysis (MFA) [15,16], and kinetic flux profiling (KFP) ...all metabolic fluxes can be inferred from the CORE profiles and the steady state isotope ...net ...
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Towards better understanding of an industrial cell factory : investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris
... C-metabolic flux analysis using 13 C-labeled substrates ...generate metabolic models of CHO cell cultures in quasi-real ...time metabolic un- derstanding, which would really impact on ...
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Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis
... Metabolic flux analysis (MFA) can provide a quantita- tive description of the metabolic network, link genome profiling to phenome analysis, and reveal pathway regula- tions through ...
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Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via 13C metabolic flux analysis
... vivo metabolic behaviors of recombinant ...stoichiometric flux analysis [24,25] and meta- bolomics analysis ...C-metabolic flux analysis ( 13 C-MFA) to systemically ...
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Bridging the gap between non-targeted stable isotope labeling and metabolic flux analysis
... detect metabolic flux changes in a non-targeted ...MID analysis to detect metabolic flux changes without considering the identity of the respec- tive ...such flux changes ...
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13C assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16
... proteome analysis (Schwartz et ...silico analysis for PHB synthesis has shown that efficient PHB synthesis can only occur under condition if carbon/nitrogen uptake ratio is up to ...network analysis ...
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13C-metabolic flux analysis of ethanol-assimilating Saccharomyces cerevisiae for S-adenosyl-l-methionine production
... the metabolic flux distribution on glu- cose and ethanol revealed that the metabolic flux through the EMP pathway was reversed by the activation of the glyoxylate shunt and gluconeogenesis ...
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Metabolic flux analysis and the NAD(P)H/NAD(P)+ ratios in chemostat cultures of Azotobacter vinelandii
... were obtained under this last condition (Table 1). These differences might be related to the protein synthesis (under high OTR) of the alternative uncoupled respira- tory chain of A. vinelandii, which has been reported ...
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Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis
... the flux redistribution of 2- oxoglutarate in the TCA cycle is not sufficient for explain- ing a change in the production activity of ...the metabolic fluxes of anaplerotic pathways under ...
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Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger
... glycolytic flux in cells by inhibiting glucokinase and glucose-6-phosphate ...glycolytic flux actually seems to depend on the redox condition of the ...in flux and metabolites pools by the ratio of ...
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Assessing and Resolving Model Misspecifications in Metabolic Flux Analysis
... low flux value ...low flux magnitude led to large mean and maximum flux biases (mean bias >150%), as highlighted in Table ...the flux estimates. In addition, removing reactions with a low ...
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OpenFLUX: efficient modelling software for 13C-based metabolic flux analysis
... square analysis. The inferred metabolic model consisted of a total of 71 reactions and 42 balanceable ...associated flux values were used deterministically as no redundant data exist in the ...
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Elucidation of the co-metabolism of glycerol and glucose in Escherichia coli by genetic engineering, transcription profiling, and 13C metabolic flux analysis
... relative flux through CS in the TCA cycle decreased in both strains with increasing dilution ...absolute flux through the TCA cycle increased in the wild type, but decreased in the ΔptsGglpK* mutant, indi- ...
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13C metabolic flux analysis-guided metabolic engineering of Escherichia coli for improved acetol production from glycerol
... with analysis of redox states revealed that NADPH regeneration is a limiting step, and the NADPH availability and well redox balance status are crucial for efficient acetol ...transhydrogenation flux from ...
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Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis
... The budding yeast S. cerevisiae is an important biotechno- logical organism e.g. for production of ethanol [1], recom- binant proteins [2], antibiotics [3] or biomass [4]. Additionally it is relevant as a model system to ...
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A 13C isotope labeling method for the measurement of lignin metabolic flux in Arabidopsis stems
... carbon flux into and within this branched metabolic ...biosynthetic flux in planta will guide our understanding of controlling steps in the pathway and facilitate future genetic manipula- tions of ...
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