Microdilution method
Novel Fluorescent Broth Microdilution Method for Fluconazole Susceptibility Testing of Candida albicans
... broth microdilution method with a novel fluorescent carboxyfluorescein diacetate (CFDA)-modified microdi- lution method for the susceptibility testing of fluconazole was conducted with 68 Candida ...
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Antifungal Susceptibility Testing of Malassezia spp with an Optimized Colorimetric Broth Microdilution Method
... We have applied this optimized broth microdilution method to test the suscepti- bility of 52 Malassezia strains to 11 antifungals. Our assay included the echinocandin class as well as griseofulvin. The data ...
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Comparison of European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Etest Methods with the CLSI Broth Microdilution Method for Echinocandin Susceptibility Testing of Candida Species
... broth microdilution (BMD) method of the European Committee on Antibiotic Susceptibility Testing (EUCAST) and the Etest agar diffusion method were compared with the Clinical and Laboratory Standards ...
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Comparison of Etest Method with Reference Broth Microdilution Method for Antimicrobial Susceptibility Testing of Yersinia pestis
... dilution) between methods was considered to be off the scale for some result comparisons (e.g., a BMD levofloxacin MIC of ⱕ 0.06 g/ml and an Etest MIC of 0.03 g/ml). At CDC, 96- well MIC trays were prepared using 100 ...
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Multisite Reproducibility of the Broth Microdilution Method for Susceptibility Testing of Nocardia Species
... broth microdilution testing on single strains of Nocardia brasiliensis, Nocardia cyriaci- georgica, Nocardia farcinica, Nocardia nova, and Nocardia ...30 microdilution panels from three different lots were ...
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<p>Comparison of Microdilution Method with Agar Dilution Method for Antibiotic Susceptibility Test of <em>Neisseria gonorrhoeae</em></p>
... tive method for clinical use to determine the antimicrobial susceptibility of ...AD method is the gold standard for quantitatively determining MICs of a batch of ...alternative method is E-test, for ...
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Evaluation of a Novel Automated Chemiluminescent Assay System for Antimicrobial Susceptibility Testing
... Antimicrobial agents. The following 22 antimicrobial agents were tested: am- picillin, penicillin G, piperacillin, oxacillin, cefazolin, cefotiam, ceftazidime, cef- podoxime, cefditoren, cefotaxime, flomoxef, aztreonam, ...
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Application of 16S rRNA Gene Sequencing To IdentifyBordetella hinzii as the Causative Agent of Fatal Septicemia
... Antimicrobial susceptibility testing was performed by the disk diffusion method, the E-test AB BIODISK, Solna, Sweden, and the broth microdilution method gram-negative panel; Dade MicroS[r] ...
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Evaluation of Alamar colorimetric broth microdilution susceptibility testing method for staphylococci and enterococci
... tinues to be a problem for many clinical microbiology labora- tories (2, 6, 8, 17, 18, 21). Testing of staphylococci against oxacillin and enterococci against vancomycin and the quino- lones can be particularly ...
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Comparison of Meropenem MICs and Susceptibilities for Carbapenemase Producing Klebsiella pneumoniae Isolates by Various Testing Methods
... Carbapenems are often used as first-line treatment for drug- resistant Gram-negative organisms, but the increasing fre- quency of carbapenemase-producing K. pneumoniae has de- creased the utility of this class of ...
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Isolation and Identification of Bacterial and Fungal Agents in patients with Corneal Ulcer in a Tertiary Care Ophthalmic Hospital.
... In the present study , Antifungal susceptibility was performed on 35 isolates for Amphotericin B and Fluconazole both by Disc Diffusion method and Broth microdilution method ( CLSI guidelines).It was ...
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Journal of Applied Pharmaceutical Science
... broth microdilution method indicated that essential oil of Pelargonium asperum exhibited significant antimicrobial activity against all tested microorganisms with the strongest inhibitory effect against ...
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Evaluation of the Revised Ceftaroline Disk Diffusion Breakpoints When Testing a Challenge Collection of Methicillin Resistant Staphylococcus aureus Isolates
... broth microdilution method correctly characterized this isolate as sus- ceptible, and 3 out 4 disk diffusion results were also correctly assigned as ...diffusion method showed a good correlation with ...
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Utility of NCCLS Guidelines for Identifying Extended Spectrum β Lactamases in Non Escherichia coli and Non Klebsiella spp of Enterobacteriaceae
... broth microdilution method (BMD), and those isolates for which the MIC of ceftazidime, cefotaxime, ceftriaxone, or aztreonam was > 2 g/ml or the MIC of cefpodoxime was > 8 g/ml (positive ESBL ...
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Comparison of Broth Microdilution, E Test, and Agar Dilution Methods for Antibiotic Susceptibility Testing of Campylobacter jejuni and Campylobacter coli
... dilution method on plates supplemented with 5% blood and cultivated under microaerophilic conditions is most often recommended [1, 19; ...dilution method is very time-consuming, it is rarely performed in ...
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Comparison of Agar Diffusion Methodologies for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients
... National Committee for Clinical Laboratory Standards (NCCLS) recommenda- tions. For Etest, the concentration ranges were 0.002 to 32 g/ml for ciprofloxacin, meropenem, and imipenem and 0.016 to 256 g/ml for all of the ...
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Cultivation of Neisseria gonorrhoeae in Liquid Media and Determination of Its In Vitro Susceptibilities to Quinolones
... dilution method, and it is recommended that direct colony suspension be used for the inoculum preparation for the susceptibility test of ...broth microdilution method and the growth method ...
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Comparison of the Etest and the Sensititre Colorimetric Methods with the NCCLS Proposed Standard for Antifungal Susceptibility Testing of Aspergillus Species
... diffusion-dilution method (the Etest method) and a colorimetric broth microdilution method (the Sensititre method); and the results were compared with those obtained by the NCCLS ...
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Emergence of Resistance of Candida albicans to Clotrimazole in Human Immunodeficiency Virus Infected Children: In Vitro and Clinical Correlations
... were tested using a noncolorimetric microdilution method. Clotrimazole concentrations ranged from 0.03 to 16 g/ml. Readings were performed after incubation for 24 and 48 h at 35°C. For 62 (71.2%) of 87 ...
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Research Paper In vitro antisalmonellal and antioxidant activities of Canarium schweinfurthii stem bark extracts
... serial microdilution method was used to determine the MIC and MBC of plants extracts against four isolates and one strain of Salmonella; the antioxidant activities of the extracts, as well as the ...
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