Microhomology-mediated end joining
Zinc finger nuclease based double strand breaks attenuate malaria parasites and reveal rare microhomology mediated end joining
... We envisaged three possible outcomes following the induced DSB (Fig. 1c). If a template of the original intact locus is present in the nucleus, repair by HR could occur, leading to parasites without genomic change. This, ...
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Microhomology-mediated end joining: new players join the team
... decreased end resection and HR effi- ...its end resection and HR function, ssDNA binding and dsDNA unwinding activities of RPA help it to inhibit MMEJ by preventing spontaneous annealing of microhomogous ...
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Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes
... Although the C-NHEJ pathway is often considered more error-prone than the HR pathway, this view has been challenged recently by emerging evidence that the latter can often be erroneous as well, especially in large and ...
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Developmental Modulation of Nonhomologous End Joining in Caenorhabditis elegans
... from microhomology-mediated rather than canonical end joining (M c V ey et ...for microhomology-mediated end joining in yeast and plants (M a et ...noncanonical ...
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Imprecision and DNA break repair biased towards incompatible end joining in leukemia
... non-homologous end joining in CLL by performing plasmid based repair assays in primary CLL cells and normal B cells as well as TALEN/Cas9 induced chromosomal deletions in the CLL cell line ...efficient ...
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DNA repair and gene targeting in plant end-joining mutants
... based end joining assays also have provided evidence for the existence of B-NHEJ ...(62;71;72). End joining is observed in the extracts of DNA-Pkcs defi cient cells and in Ku-depleted ...DNA ...
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Structural and Functional Characterization of Non-Homologous End Joining Factors
... DNA repair provides the path for all organisms to maintain genome stability against a plethora of damaging agents. The two major DSB repair pathways in mammals are homologous recombination (HR) and non-homologous ...
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Ku DNA End-Binding Activity Promotes Repair Fidelity and Influences End-Processing During Nonhomologous End-Joining in Saccharomyces cerevisiae
... nonhomologous end-joining (NHEJ) of DNA double-strand breaks ...DNA end-binding ...Ku-independent microhomology-mediated end- joining (MMEJ) and were neither promoted by ...
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PubMedCentral-PMC5466332.pdf
... named microhomology-mediated end joining (MMEJ) and is also a critical component for bypass or repair of ICLs in several ...overall end-joining efficiency is not affected in ...
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Wyatt_unc_0153D_16692.pdf
... on microhomology associated deletions is comparable to deficiency in a factor required for end resection, Mre11 (Figure ...chromosomal end joining products still accumulate in Polq -/- Ku70 ...
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Efficient Gene Replacements in Toxoplasma gondii Strains Deficient for Nonhomologous End Joining
... nonhomologous end-joining (NHEJ) DNA repair pathway could be disrupted in this obligate intracellular parasite, putative KU proteins were identified and a predicted KU80 gene was ...
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Heat shock factor 1, an inhibitor of non-homologous end joining repair
... The heat shock factor (HSF)1 activation is critical for maintaining homeostasis of the proteomes of cells and is mediated in large part by increased expression of classical heat shock proteins (HSP) such as HSP27, ...
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CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome
... remodeler cause immunodeficiency, centromeric instability, and facial anomalies (ICF) syndrome types 3 and 4. Here, we demonstrate that the classical nonhomologous end joining (C-NHEJ) proteins Ku80 and ...
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Overcoming obstacles to nonhomologous end joining repair of chromosome double strand breaks
... to-tail joining was assessed by semi-quantitative polymerase chain ...in joining the undamaged substrate when compared to the substrate with an embedded normal AP site (Figure ...frequent joining ...
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Merotelic attachments and non-homologous end joining are the basis of chromosomal instability
... homologous end joining or homologous recombination repair mutants have problems repairing induced DSB, the inactivation of a single repair pathway does not result in spontaneous DSB accumulation ...
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Positive selection on the nonhomologous end-joining factor Cernunnos-XLF in the human lineage
... Cernunnos-XLF is a new component of the nonhomolo- gous end-joining machinery mutated in human immuno- deficiency with microcephaly [4,5]. Using newly obtained coding sequences in chimpanzee and rhesus ...
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Analysis of turbidity progress curves from protein aggregation reactions
... nucleus and adopting the template structure encoded by it (Jarrett and Lansbury 1992; Petkova et al. 2005; Wetzel 2006). As the amyloid reaction proceeds, fibres can break apart (Hall and Edskes 2009, 2012, 2004; Xue et ...
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Multiple Barriers to Nonhomologous DNA End Joining During Meiosis in Drosophila
... Precondition genes such as mei-218 and rec have generally been defined by two mutant phenotypes: a reduction in the frequency of crossovers and an altered distribution of resid- ual events that is more proportional to the ...
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The non-homologous end-joining activity is required for Fanconi anemia fetal HSC maintenance
... A clinical application of HR-NHEJ interaction is syn- thetic lethality induced by poly (ADP-ribose) polymerase (PARP) inhibition in BRCA1/2-mutated cancer [17, 18]. Since PARP functions as a critical sensor of ...
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The akuBKU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus
... homologous end joining, involving direct ligation of the strand ends independently of DNA homology ...nonhomologous end joining. The nonhomologous end-joining process is medi- ...
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