Modification of alternative splicing using splice switching
Alternative splicing in prostate cancer.
... specific splicing events, to prevent their alternate splicing and the production of pathological erroneous protein ...these splicing events are more diverse, evidence in support of this approach ...
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Chromatin, DNA structure and alternative splicing
... For years, a simplistic model has been accepted referring to RNAPII phosphorylation, according to which Ser5 phosphorylation (Ser5P) by Cdk7, as part of TFIIH was a hallmark of transcription initiation, whereas Ser2P by ...
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Analysis of alternative splicing events for cancer diagnosis using a multiplexing nanophotonic biosensor
... BiMW biosensor biofunctionalization. Once assessed the proper conditions for the sensor biofunction- alization, we evaluated the analytical performance of the BiMW device for the direct detection of Fas gene iso- forms. ...
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The Complexity of the IGF1 Gene Splicing, Posttranslational Modification and Bioactivity
... the alternative splicing of its pre-mRNA both at the 5′ and 3′ end, and the isoform-specific co- and posttransla- tional modifications, which appear to play key roles in modulating the bio - availabilty and ...
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Cross kingdom patterns of alternative splicing and splice recognition
... While ED is most common in animals and ID dominates in fungi and protists, nearly all species analyzed show evidence for using both mechanisms. ID and ED have previously been shown to operate within the same ...
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The usage of exon-exon splice junctions for the detection of alternative splicing using the REIDS model.
... TAC and AltAnalyze based on a more recent HTA-2.0 microarray platform. The REIDS method performs as good AltAnalyze, and is less prone to false positives than TAC. The presence of exon-exon junctions provides valuable ...
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RNA-Seq analysis of splicing in Plasmodium falciparum uncovers new splice junctions, alternative splicing and splicing of antisense transcripts.
... truncating alternative splicing events in ...Regulated splicing controlling the ratio of NMD-targeted to protein-coding isoform produced from certain genes is a mechanism of post-transcriptional ...
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Splicing factor SRSF1 promotes gliomagenesis via oncogenic splice switching of MYO1B
... 32). Using EGFP fusion proteins, we pro- vided evidence that EGFP-fused MYO1B-fl localized mainly on the cytomembrane, while EGFP-fused MYO1B-t was dispersed in the ...RNA splicing and the decrease of the ...
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The Alternative Splicing Mutation Database: a hub for investigations of alternative splicing using mutational evidence
... of splice junctions. Splicing effects of the mutations are quantified by the relative presence of alternative mRNA isoforms with and without a given ...
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Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5′ splice site strength
... By using spliceosome iCLIP, we demon- strate that the specificity of PRPF8 depletion on splicing events strikingly correlates with both weaker 5′ splice sites as well as decreased interaction of the ...
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A study of alternative splicing in the pig
... database. Using the Distiller package 2,515 EST clusters with candidate alternative isoforms were identified in the EST data with high ...putative splice variants in about 30% of the contigs with ...
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Pharmacology of Modulators of Alternative Splicing
... splicing is the ...assay, using a high-throughput screen. For alternative splic- ing, the key point is whether the alternative splicing is modulated, and the target may not be ...
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Alternative Splicing in Apicomplexan Parasites
... parasite splicing processes without affecting human ...native splicing for relatively few genes (only several percent) compared to well- characterized metazoans ( ⬎ 90%), but this may be confounded by use ...
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Pharmacology of modulators of alternative splicing
... disrupt splicing of many genes, and the therapeutic window is therefore ...narrower. Using oligo-targeted therapies gives a more subtle biomarker, because it is possible that only a single RNA species may ...
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Alternative splicing in lung cancer
... reaction using splice variant-specific ...particular splice variants could be generated and applied in ...identify splicing patterns of many genes simultaneously, will undoubtedly push forward ...
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Reprogramming Alternative Splicing Using Cis Acting Intronic Control Elements
... differential splicing patterns (i.e., tissue specific splicing), a dominant factor guiding function is likely the immediate transcript context in which these cellular factors ...the splicing code ...
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Alternative splicing programs in prostate cancer
... SMN2 splicing in vitro and in vivo and profoundly ameliorated the viability and phenotypic features of mice affected by a severe form of SMA [ 75 ...BCL-X splicing toward the proapoptotic variant was ...
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The fitness cost of mis splicing is the main determinant of alternative splicing patterns
... lines, using 110 publicly available RNA-seq sam- ples (Additional file 1: Table S3), corresponding to a representative subset of the samples analyzed by Braun- schweig et ...84) using TopHat ...the ...
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Cross-kingdom patterns of alternative splicing and splice recognition
... of splice variation The varying prevalence of CEs and RIs across major eukaryo- tic groups raises evolutionary questions about modern varia- tion in splice recognition among lineages, as well as the nature ...
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Mutations in the regions of the Rous sarcoma virus 3' splice sites: implications for regulation of alternative splicing.
... Thus, each of the mutations that we studied in the region of the env 3'ss resulted in changes in the relative levels of spliced env and src mRNAs but did not change the overall extent of[r] ...
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