mouse embryonic fibroblasts
Transcriptional regulatory program in wild type and retinoblastoma gene deficient mouse embryonic fibroblasts during adipocyte differentiation
... wild-type mouse embryonic fibroblasts (MEFs) undergoing adipogenesis and com- pared these expression data with microarray gene expression data from 3T3-L1 cell adipogenesis to iden- tify a ...
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Automatic segmentation of focal adhesions from mouse embryonic fibroblasts
... This work describes an automatic algorithm for the segmentation and quantification of focal adhesions from mouse embryonic fibroblasts. The main challenges solved by this algorithm are: the ...
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Variation in Antagonism of the Interferon Response to Rotavirus NSP1 Results in Differential Infectivity in Mouse Embryonic Fibroblasts
... primary mouse embryonic fibroblasts (MEFs) from wild-type or IFN-deficient (IFNR KO or STAT1 KO) mice to investigate the different roles of the IFN system in regulating the replication of selected ...
8
Amino acid substitutions in the E2 glycoprotein of Sindbis like virus XJ 160 confer the ability to undergo heparan sulfate dependent infection of mouse embryonic fibroblasts
... of mouse embryonic fibro- blasts (MEF) ...wild-type mouse embryonic fibroblasts (MEF-wt) or MEF-Epi -/- cells which produce mutant ...
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Identification of the lncRNA, AK156230, as a novel regulator of cellular senescence in mouse embryonic fibroblasts
... in mouse embryonic fibroblasts (MEFs), and knockdown of AK156230 promotes a robust senescence phenotype, including increase in the numbers of the senescence-associated β-galactosidase-positive cells, ...
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Calendula officinalis stimulate proliferation of mouse embryonic fibroblasts via expression of growth factors TGFβ1 and bFGF
... of mouse embryonic fibroblasts (MEFs) were performed according to the protocol described elsewhere ...pregnant mouse at 13 or 14 days post-coitum ...
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Direct conversion of mouse embryonic fibroblasts into functional keratinocytes through transient expression of pluripotency-related genes
... from embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), or somatic cells by combinations of specific ...of mouse embryonic fibroblasts into functional keratinocytes via the ...
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Reprogramming of Mouse Embryonic Fibroblasts to Mouse Hematopoietic Progenitor Cells
... study, mouse embryonic fibroblasts (MEFs) were reprogrammed into hematopoietic progenitor cells (HPCs) in long-term HSC culture medium by co-infecting the MEFs with ...
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Characterisation of extraembryonic endoderm-like cells from mouse embryonic fibroblasts induced using chemicals alone
... of somatic cells into iPSCs with Oct4 alone [38], which could be replaced by the synergistic effect of forskolin and DZNep in Hou’s experiments [6]. Despite its potential to substitute for Oct4, DZNep has no effect on ...
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Replication of Hepatitis C Virus (HCV) RNA in Mouse Embryonic Fibroblasts: Protein Kinase R (PKR)-Dependent and PKR-Independent Mechanisms for Controlling HCV RNA Replication and Mediating Interferon Activities
... numerous mouse genes have al- ready been well defined for their roles in the host innate immunity and IFN signaling and that a variety of MEFs de- rived from diverse gene knockout mice are readily ...transgenic ...
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E2F2 directly regulates the STAT1 and PI3K/AKT/NF κB pathways to exacerbate the inflammatory phenotype in rheumatoid arthritis synovial fibroblasts and mouse embryonic fibroblasts
... STAT1 is a member of the STAT family of transcrip- tion factors. STAT1 expression was reported to be sig- nificantly higher in RA synovial tissue than in OA and mandatory spondylitis [3]. In addition, functionalized ...
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FRS2α is Essential for the Fibroblast Growth Factor to Regulate the mTOR Pathway and Autophagy in Mouse Embryonic Fibroblasts
... the mouse syndecan 1 gene (15), which is an enhancer required for the FGF to promote syndecan 1 expression and has been widely used as a reporter for the FGF signaling pathway ...
8
ZFAT is a critical molecule for cell survival in mouse embryonic fibroblasts
... embryonic day (E) 8.5, with a reduction in the number of blood islands and hematopoietic progenitor cells. ZFAT directly regulates the transcription factors including Tal1, Lmo2 and Gata1 in the blood islands [6]. ...
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Genetic background affects induced pluripotent stem cell generation
... Methods: Mouse embryonic fibroblasts (MEFs) were isolated from six strains of mice (NON/LtJ; C57BL/6J; DBA/2J; BALB/cJ; 129S1/SvlmJ; CAST/EiJ) that were selected based on genetic diversity and ...
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Lipid–peptide bioconjugation through pyridyl disulfide reaction chemistry and its application in cell targeting and drug delivery
... for the tbFGFp mediated cellular uptake of liposomes for cell-to-cell communication and their use in drug delivery [22, 46–51]. The GALA peptide was designed to mimic viral fusion protein sequences that interact with ...
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c-IAP1 binds and processes PCSK9 protein: linking the c-IAP1 in a TNF-alpha pathway to PCSK9-mediated LDLR degradation pathway
... null mouse embryonic fibroblasts (MEFs), there is a dramatic decrease in secreted mature PCSK9 protein, while in the wild-type MEF cells, proprotein form of the PCSK9 was properly processed and ...
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Reprogramming fibroblasts to pluripotency using arginine-terminated polyamidoamine nanoparticles based non-viral gene delivery system
... from mouse embryonic fibroblasts (MEFs) using arginine-terminated generation 4 polyamidoamine (G4Arg) nanoparticles as a nonviral trans- fection vector for the delivery of a single plasmid construct ...
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Advances in understanding the cell types and approaches used for generating induced pluripotent stem cells
... ESCs: Embryonic stem cells; MEFs: Mouse embryonic fibroblasts; TTFs: Tail-tip fibroblasts; O: Oct 3/4; S: Sox2; K: Klf4: M, c-Myc; hiPSCs: Human iPSCs; HDFs: Human dermal ...
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Mouse peritoneal cells confer an antiviral state on mouse cell monolayers: role of interferon.
... The above experiments showed that the capacity of peritoneal cells to confer an antiviral state on target monolayer cultures of mouse embryonic fibroblasts or aged mouse macrophages was [r] ...
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Zannas, Antonios (2017): Epigenetic mechanisms linking psychological stress and aging-related disease. Dissertation, LMU München: Fakultät für Biologie
... human embryonic kidney (HEK) 293 cells, expression of FKBP51 displaced FKBP52 from CDK5, decreased the interaction of CDK5 with DNMT1, reduced the phosphorylation and enzymatic activity of DNMT1, and diminished ...
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