Mre11/Rad50/Nbs1
Ataxia Telangiectasia-Mutated Damage-Signaling Kinase- and Proteasome-Dependent Destruction of Mre11-Rad50-Nbs1 Subunits in Simian Virus 40-Infected Primate Cells
... Although the mechanism of simian virus 40 (SV40) DNA replication has been extensively investigated with cell extracts, viral DNA replication in productively infected cells utilizes additional viral and host functions ...
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The Mre11/Rad50/Nbs1 Complex Limits Adeno-Associated Virus Transduction and Replication
... Purified Mre11 complex components have been shown to recognize a variety of DNA structures, including hairpins ...rified Mre11 and Nbs1 proteins were obtained and tested for association with the ITRs ...
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Insight into the Mechanism of Inhibition of Adeno-Associated Virus by the Mre11/Rad50/Nbs1 Complex
... in Mre11 Cond/H129N ...the Mre11 H129N protein is stably expressed, interacts with Rad50 and Nbs1, and assembles in foci at sites of DNA ...rAAV, Mre11 Cond/⫹ , Mre11 Cond/⫺ , ...
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Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe
... the Mre11-Rad50-Nbs1 (MRN) complex has known roles in repair processes like homologous recombination and microhomology-mediated end-joining, its role in nonhomologous end-joining (NHEJ) is unclear as ...
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Adenovirus Regulates Sumoylation of Mre11-Rad50-Nbs1 Components through a Paralog-Specific Mechanism
... of Nbs1 and SUMO-2 conjugation of ...and Mre11 and Nbs1 were analyzed by Western ...moylated Mre11 and Nbs1 from lysates of Ad5-infected SUMO- 2-expressing HeLa cells ...to Nbs1, ...
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A Novel Recombination Pathway Initiated by the Mre11/Rad50/Nbs1 Complex Eliminates Palindromes During Meiosis in Schizosaccharomyces pombe
... served Mre11/Rad50/Nbs1 (MRN) complex is likely directly involved in processing palindromes through the homologous recombination pathway of DNA ...
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Relocalization of the Mre11-Rad50-Nbs1 Complex by the Adenovirus E4 ORF3 Protein Is Required for Viral Replication
... Our data suggest a model of infection that requires the control and sequestration of the MRN complex for efficient virus growth. At early times after infection, wild-type E4 ORF3 reorganizes MRN components in a manner ...
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Temporal Regulation of the Mre11-Rad50-Nbs1 Complex during Adenovirus Infection
... Immunofluorescence. A549 and HeLa cells were seeded on glass coverslips and transfected according to the manufacturer’s instructions using FuGene 6 (Roche) and/or infected as described above. In some cases, the cells ...
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Möckel, Carolin (2011): Structural and functional analysis of ATP dependent conformational changes in the bacterial Mre11:Rad50 catalytic head complex. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... The Mre11:Rad50:Nbs1 (MRN; MRX in ...Disregarding Nbs1, which is restricted to eukaryotes, MR has conserved morphological features and enzymatic activities, suggesting a conserved framework ...
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Serotype-Specific Reorganization of the Mre11 Complex by Adenoviral E4orf3 Proteins
... the Mre11 complex (35), it would seem that both proteins perform this function by tar- geting components of this repair ...the Mre11/Rad50/Nbs1 proteins by the E1b55K/E4orf6 complex is ...
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Aspergillus nidulans uvsBATR and scaANBS1 Genes Show Genetic Interactions during Recovery from Replication Stress and DNA Damage
... The Mre11 (Mre11-Rad50-Nbs1) protein complex has emerged as a central player in the human cellular DNA dam- age response, and recent observations suggest that these pro- teins are at least ...
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Ataxia-telangiectasia Mutated Kinase (ATM) as a Central Regulator of Radiation-induced DNA Damage Response
... which induces dissociation of an inactive dimer and trig- gers ATM activity (1). Also a specific protein complex is re- quired for its activation – MRN complex consisting of Mre11, Rad50, and Nbs1 ...
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Post-transcriptional regulation of MRE11 expression in muscle-invasive bladder tumours.
... with RAD50 and NBS1, and stability of the three MRN proteins is linked, with MRE11 mutations which destabilise the MRN complex being associated with reduced RAD50 and NBS1 protein ...
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The Drosophila Nbs Protein Functions in Multiple Pathways for the Maintenance of Genome Stability
... and rad50 nbs double mutants are significantly higher than those observed in the corresponding single mutants (Table ...ATM/ Rad50/Mre11 ...homolog), Rad50, Mre11, and Xrs2 (the NBS ...
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The Mre11p/Rad50p/Xrs2p Complex and the Tel1p Function in a Single Pathway for Telomere Maintenance in Yeast
... Paull, T. T., and M. Gellert, 1999 Nbs1 potentiates ATP-driven rates in transcriptionally active DNA. Cell 56: 619–630. DNA unwinding and endonuclease cleavage by the Mrell/Rad50 Usui, T., T. Ohta, H. ...
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The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius
... conserved Rad50 and Mre11 proteins must play important roles in these processes even if their precise function is still ...Eucarya, Rad50 and Mre11 proteins are associated with a third ...
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Mutations in Mre11 Phosphoesterase Motif I That Impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 Complex Stability in Addition to Nuclease Activity
... HE RAD50, XRS2, and MRE11 genes were first iden- tified in screens for ionizing radiation (IR)-sensitive or meiotic recombination-defective mutants in Saccha- romyces cerevisiae (S ymington ...in ...
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hSSB1 rapidly binds at the sites of DNA double strand breaks and is required for the efficient recruitment of the MRN complex
... of Mre11 and Rad50 (Supplementary Figure ...for Mre11 (AT-LD) or NBS1 (ILB1) (26,27) also failed to show a dramatic effect on hSSB1 foci formation or chromatin loading (Figure 4c, ...
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Overlapping Functions of the Saccharomyces cerevisiae Mre11, Exo1 and Rad27 Nucleases in DNA Metabolism
... or mre11-H125N ...exo1⌬ mre11⌬ strain, but switching ends to provide a single-stranded substrate for the efficiency was not restored to wild-type ...levels. Mre11 endonuclease ...including ...
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Differential Requirements of the C Terminus of Nbs1 in Suppressing Adenovirus DNA Replication and Promoting Concatemer Formation
... of Mre11, Rad50, and Nbs1 (MRN) is required for concatemer formation and full activation of damage signaling through the protein kinases Ataxia-telan- giectasia mutated (ATM) and ATM-Rad3-related ...
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