MRE11-RAD50-NBS1 COMPLEX
A Novel Recombination Pathway Initiated by the Mre11/Rad50/Nbs1 Complex Eliminates Palindromes During Meiosis in Schizosaccharomyces pombe
... tative complex has properties similar to those of the originated through a pathway that did not involve reso- Rad1/Rad10 complex (Carr et al. 1994). lution of a H J by the Mus81/Eme1 complex. Hence, ...
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Insight into the Mechanism of Inhibition of Adeno-Associated Virus by the Mre11/Rad50/Nbs1 Complex
... the complex itself. Several functions of the MRN complex can be attributed to subunit pro- teins and, in some cases, distinct domains mapped within those ...the Mre11 protein ...
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Relocalization of the Mre11-Rad50-Nbs1 Complex by the Adenovirus E4 ORF3 Protein Is Required for Viral Replication
... Immunofluorescence analysis. A549 cells were placed on coverslips and in- fected as described above. At 6, 9, 12, and 15 h postinfection, the cells were fixed with ⫺ 20°C methanol for 5 min. The cells were washed with ...
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The Mre11/Rad50/Nbs1 Complex Limits Adeno-Associated Virus Transduction and Replication
... Degradation of MRN enhances rAAV transduction. The Ad E1b55K/E4orf6 protein complex has been shown to enhance rAAV transduction dramatically (19, 20, 24). We wished to determine whether destabilization of a ...
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Temporal Regulation of the Mre11-Rad50-Nbs1 Complex during Adenovirus Infection
... Immunofluorescence. A549 and HeLa cells were seeded on glass coverslips and transfected according to the manufacturer’s instructions using FuGene 6 (Roche) and/or infected as described above. In some cases, the cells ...
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Adenovirus Type 5 E4orf3 Protein Targets the Mre11 Complex to Cytoplasmic Aggresomes
... the Mre11/Rad50/Nbs1 ...the Mre11 complex in nuclear tracks and also in large cytoplasmic ...of Mre11 and Rad50 by Ad5 E4orf3 is not dependent on interactions with ...
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Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe
... ctp1D mre11-H134S double mutant showed wild-type levels of excision (Figure 6 and Figure ...quently, Mre11, Ctp1, and Pso2 do not appear to be the major nucleases that open the hairpin for further ...this ...
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The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius
... conserved Rad50 and Mre11 proteins must play important roles in these processes even if their precise function is still ...Eucarya, Rad50 and Mre11 proteins are associated with a third ...
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The Mre11p/Rad50p/Xrs2p Complex and the Tel1p Function in a Single Pathway for Telomere Maintenance in Yeast
... 1999 Nbs1 potentiates ATP-driven rates in transcriptionally active ...al., complex. Genes Dev. 13: 1276–1288. 1998 Complex formation and functional versatility of Mre11 of Porter, ...
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The Drosophila Nbs Protein Functions in Multiple Pathways for the Maintenance of Genome Stability
... and rad50 nbs double mutants are significantly higher than those observed in the corresponding single mutants (Table ...ATM/ Rad50/Mre11 ...homolog), Rad50, Mre11, and Xrs2 (the NBS ...
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Adenovirus Regulates Sumoylation of Mre11-Rad50-Nbs1 Components through a Paralog-Specific Mechanism
... he Mre11-Rad50-Nbs1 (MRN) complex is a sensor and effec- tor of the DNA damage response (DDR) and plays an impor- tant role in DNA repair pathways (reviewed in reference ...teins. Mre11 ...
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Mutations in Mre11 Phosphoesterase Motif I That Impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 Complex Stability in Addition to Nuclease Activity
... HE RAD50, XRS2, and MRE11 genes were first iden- tified in screens for ionizing radiation (IR)-sensitive or meiotic recombination-defective mutants in Saccha- romyces cerevisiae (S ymington ...in ...
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Ataxia Telangiectasia-Mutated Damage-Signaling Kinase- and Proteasome-Dependent Destruction of Mre11-Rad50-Nbs1 Subunits in Simian Virus 40-Infected Primate Cells
... and Mre11-Rad50-Nbs1 (MRN) assemble with T antigen and other viral DNA replication proteins in large nuclear ...MRN complex, suggesting that MRN may undergo proteasome-dependent ...
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Post-transcriptional regulation of MRE11 expression in muscle-invasive bladder tumours.
... MRN complex with RAD50 and NBS1, and stability of the three MRN proteins is linked, with MRE11 mutations which destabilise the MRN complex being associated with reduced RAD50 and ...
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Möckel, Carolin (2011): Structural and functional analysis of ATP dependent conformational changes in the bacterial Mre11:Rad50 catalytic head complex. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... The Mre11:Rad50:Nbs1 (MRN; MRX in ...bacteria) complex describes such a keystone complex, involved in DSB sensing, nucleolytic processing of DNA ends, recruitment of DSB processing ...
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Ataxia-telangiectasia Mutated Kinase (ATM) as a Central Regulator of Radiation-induced DNA Damage Response
... protein complex is re- quired for its activation – MRN complex consisting of Mre11, Rad50, and Nbs1 protein ...MRN complex and that mu- tation of its components leads to genetic ...
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Overlapping Functions of the Saccharomyces cerevisiae Mre11, Exo1 and Rad27 Nucleases in DNA Metabolism
... or mre11-H125N ...MRX complex unwinds type switching of the exo1⌬ mre11⌬ strain, but switching ends to provide a single-stranded substrate for the efficiency was not restored to wild-type ...levels. ...
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Coordinate Regulation of DNA Damage and Type I Interferon Responses Imposes an Antiviral State That Attenuates Mouse Gammaherpesvirus Type 68 Replication in Primary Macrophages
... MRN complex consisting of the Mre11, Nbs1, and Rad50 proteins (reviewed in reference ...This complex is highly conserved in eukaryotes ranging from yeast to mammals, and mutations in ...
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High expression of MRE11 correlates with poor prognosis in gastric carcinoma
... The MRE11/RAD50/NBS1 (MRN) complex plays a cen- tral role in most aspects of the cellular response to DSBs, including HR, NHEJ, telomere activity and DNA damage checkpoint activation [9 – ...
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hSSB1 rapidly binds at the sites of DNA double strand breaks and is required for the efficient recruitment of the MRN complex
... MRN complex is thought to be the sensor and initiator of the DSB response pathway, and activates ATM and downstream DSB signalling, by tethering broken DNA ends together and recruiting ATM which facilitates ATM ...
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