Mre11/Rad50/Xrs2
Mutations in Mre11 Phosphoesterase Motif I That Impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 Complex Stability in Addition to Nuclease Activity
... HE RAD50, XRS2, and MRE11 genes were first iden- tified in screens for ionizing radiation (IR)-sensitive or meiotic recombination-defective mutants in Saccha- romyces cerevisiae (S ymington ...in ...
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Differential Suppression of DNA Repair Deficiencies of Yeast rad50, mre11 and xrs2 Mutants by EXO1 and TLC1 (the RNA Component of Telomerase)
... Rad50, Mre11, and Xrs2 form a nuclease complex that functions in both nonhomologous end-joining (NHEJ) and recombinational repair of DNA double-strand breaks ...of rad50 mutants yielded ...
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Alteration of N-Terminal Phosphoesterase Signature Motifs Inactivates Saccharomyces cerevisiae Mre11
... by mre11 mutants with respect to mitotic interhomologue hyperrecombi- nation, IR sensitivity, and DSB repair deficiency suggests that these phenotypic endpoints arose from similar DNA ...cerevisiae ...
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The Saccharomyces cerevisiae mre11(ts) Allele Confers a Separation of DNA Repair and Telomere Maintenance Functions
... yeast Mre11 protein participates in important cellular functions such as DNA repair and telomere ...of Mre11 has led to identification of several separation-of-function mutations as well as N- and ...
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Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe
... called Mre11- Rad50-Xrs2 (MRX), NHEJ requires MRX in an end-joining assay that monitors recircularization of restriction enzyme- cut plasmids transformed into yeast (Zhang and Paull 2005) or one that ...
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Multiple Endonucleases Function to Repair Covalent Topoisomerase I Complexes in Saccharomyces cerevisiae
... and Mre11 participate in repairing campto- thecin-induced damage: Sae2 was originally identified by its requirement in meiotic recombination, a require- ment that can be bypassed by a mutation in SPO11 (McKee and ...
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Overlapping Functions of the Saccharomyces cerevisiae Mre11, Exo1 and Rad27 Nucleases in DNA Metabolism
... vitro, Mre11 is implicated in the 5⬘ to 3⬘ resection of duplex ends in ...The mre11-H125N mutation, which eliminates the nuclease activities of Mre11, causes an accumulation of unprocessed ...
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The Drosophila Nbs Protein Functions in Multiple Pathways for the Maintenance of Genome Stability
... proteins Mre11 and Rad50 and a third less- conserved component, Nbs/Xrs2 (D’A mours and J ackson 2002; A ssenmacher and H opfner ...and Mre11 genes lead to the Nijmegen breakage syndrome (NBS) ...
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The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius
... conserved Rad50 and Mre11 proteins must play important roles in these processes even if their precise function is still ...Eucarya, Rad50 and Mre11 proteins are associated with a third ...
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The Mre11/Rad50/Nbs1 Complex Limits Adeno-Associated Virus Transduction and Replication
... The Mre11 complex (MRN), composed of Mre11, Rad50, and Nbs1 (Xrs2 in Saccharomyces cerevisiae), is a conserved multi- protein complex that is also critical to DNA damage sensing, signaling, ...
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Role of the Nuclease Activity of Saccharomyces cerevisiae Mre11 in Repair of DNA Double-Strand Breaks in Mitotic Cells
... The Rad50:Mre11:Xrs2 (RMX) complex functions in repair of DNA double-strand breaks (DSBs) by recombination and nonhomologous end-joining (NHEJ) and is also required for telomere ...The Mre11 ...
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Conserved and Nonconserved Proteins for Meiotic DNA Breakage and Repair in Yeasts
... agar supplemented with all required nutrients. Haploid pat1- controlling factor, complexed with Rad50•Mre11•Xrs2 114 strains were grown and induced for meiosis as described is responsible for meiotic ...
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The Mre11p/Rad50p/Xrs2p Complex and the Tel1p Function in a Single Pathway for Telomere Maintenance in Yeast
... plex could increase accessibility of telomeric DNA to telomerase. Two further points should be mentioned. First, Tel1p and the MRX complex could affect accessi- bility of the telomere to cellular exonucleases as well as ...
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Ataxia Telangiectasia-Mutated Damage-Signaling Kinase- and Proteasome-Dependent Destruction of Mre11-Rad50-Nbs1 Subunits in Simian Virus 40-Infected Primate Cells
... Although the mechanism of simian virus 40 (SV40) DNA replication has been extensively investigated with cell extracts, viral DNA replication in productively infected cells utilizes additional viral and host functions ...
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Relocalization of the Mre11-Rad50-Nbs1 Complex by the Adenovirus E4 ORF3 Protein Is Required for Viral Replication
... against Mre11 (B), Rad50 (F), Nbs1 (J), and PML (N) (MRNP) and against E4 ORF3 (C, G, K, and ...O). Mre11, Rad50, Nbs1, and PML were visualized with FITC-labeled secondary antibody, and E4 ORF ...
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DNA damage induces nucleoid compaction via the Mre11 Rad50 complex in the archaeon Haloferax volcanii
... the Mre11-Rad50 complex, suggesting a link to DNA double-strand break ...of Mre11-Rad50 in nucleoid reorganisation is independent of homolo- gous ...
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Serotype-Specific Reorganization of the Mre11 Complex by Adenoviral E4orf3 Proteins
... cellular Mre11 complex and is prevented by both the E4orf3 and E4orf6 ...The Mre11/Rad50/Nbs1 proteins are targeted for proteasome-mediated degradation by the Ad5 viral E1b55K/E4orf6 ...of ...
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Mimivirus reveals Mre11/Rad50 fusion proteins with a sporadic distribution in eukaryotes, bacteria, viruses and plasmids
... these Mre11/ Rad50-like fusion proteins as well as in their subsequent propagation into different cellular ...of Mre11/Rad50 homologs from viruses (such as gp46/gp47 of T4 and other marine ...
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A Novel Recombination Pathway Initiated by the Mre11/Rad50/Nbs1 Complex Eliminates Palindromes During Meiosis in Schizosaccharomyces pombe
... Adair, G. M., R. L. Rolig, D. Moore-Faver, M. Zabelshansky, J. H. Haber, J. E., 1998 The many interfaces of Mre11. Cell 95: 583–586. Wilson et al., 2000 Role of ERCC1 in removal of long non- Hochstenbach, F., F. ...
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Ataxia-telangiectasia Mutated Kinase (ATM) as a Central Regulator of Radiation-induced DNA Damage Response
... Exposure of mammalian cells to physical or chemical factors can result in DNA lesions as intermolecular cross- linking, single or double strand breaks and others (23). Double strand breaks (DSB) cause chromatin ...
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