mRNA capping
5′-Phospho-RNA Acceptor Specificity of GDP Polyribonucleotidyltransferase of Vesicular Stomatitis Virus in mRNA Capping
... The PRNTase domains of NNS RNA viral L proteins contain five conserved amino sequence elements, Rx(3)Wx(3– 8) ⌽ xGx x(P/A) (motif A; ⌽ , hydrophobic; , hydrophilic), (Y/W) ⌽ GSxT (motif B), W (motif C), HR (motif D), ...
11
A Conserved Motif in Region V of the Large Polymerase Proteins of Nonsegmented Negative-Sense RNA Viruses That Is Essential for mRNA Capping
... the mRNA cap plays a key role in controlling polymerase ...the mRNA capping machinery requires specific signals in the RNA (29, 42) and that mRNA cap methylation is accomplished by two ...
10
Structure-function analysis of the triphosphatase component of vaccinia virus mRNA capping enzyme.
... Conclusions and implications. We have identified five indi- vidual amino acid residues in the N-terminal half of D1(1-545) that are essential for the triphosphatase activity of vaccinia virus mRNA capping ...
7
A Yeast-Based Genetic System for Functional Analysis of Viral mRNA Capping Enzymes
... Virus-encoded mRNA capping enzymes are attractive targets for antiviral therapy, but functional studies have been limited by the lack of genetically tractable in vivo systems that focus exclusively on the ...
9
Human Parainfluenza Virus Type 2 L Protein Regions Required for Interaction with Other Viral Proteins and mRNA Capping
... also mutated this residue to Q and M to introduce a much bulkier side chain at this position (Fig. 5A, rows 3 and 4). Both of these point mutations also eliminated GFP expression (Fig. 5B, lanes 3 and 4). Thus, a domain ...
8
Deletion of Individual mRNA Capping Genes Is Unexpectedly Not Lethal to Candida albicans and Results in Modified mRNA Cap Structures
... in mRNA capping, especially the mRNA triphos- phatase, have been proposed as potentially good antifungal targets because they are significantly different from the human enzymes and are reportedly ...
11
Formation of Guanosine(5′)Tetraphospho(5′)Adenosine Cap Structure by an Unconventional mRNA Capping Enzyme of Vesicular Stomatitis Virus
... of mRNA through the 5 ⬘ -5 ⬘ triphosphate bridge, is cotrans- criptionally formed by a series of enzymatic steps and plays essential roles in various stages of mRNA metabolism, includ- ing translation and ...
6
Vesicular Stomatitis Virus mRNA Capping Machinery Requires Specific cis-Acting Signals in the RNA
... for mRNA initiation and mRNA cap ...the mRNA capping appa- ratus functions in a sequence-specific ...the mRNA capping machinery of the NNS RNA viruses, which renders them at- ...
8
Mutational analysis of the RNA triphosphatase component of vaccinia virus mRNA capping enzyme.
... virus mRNA capping enzyme is a multifunctional protein with RNA triphosphatase, RNA gua- nylyltransferase, and RNA (guanine-7-) methyltransferase ...the capping of triphosphate-terminated poly(A) but ...
7
mRNA Capping by Venezuelan Equine Encephalitis Virus nsP1: Functional Characterization and Implications for Antiviral Research
... alphavirus mRNA capping is an attractive target for the design of specific ...RNA capping by ribavirin 5=-triphosphate (data not shown for the last step), we can exclude that its antiviral effect is ...
12
RNA Triphosphatase Component of the mRNA Capping Apparatus of Paramecium bursaria Chlorella Virus 1
... eukaryotic mRNA is formed cotranscriptionally by three enzymatic reactions: (i) the 5⬘ tri- phosphate end of the nascent RNA is hydrolyzed to a diphos- phate by RNA triphosphatase (RTP), (ii) the diphosphate end ...
7
mRNA capping enzymes are masked in reovirus progeny subviral particles.
... 490-496 0022-538X/80/05-0490/07$02.00/0 mRNA Capping Enzymes Are Masked in Reovirus Progeny Subviral Particles DANIAL SKUP AND STEWART MILLWARD* Department of Biochemistry, McGill Univer[r] ...
7
c-Myc deregulation induces mRNA capping enzyme dependency
... 59]. Recent studies reported that many cancer cell lines are sensitive to Cdk7 inhibitors, which reduce RNA pol II Ser-5 phosphorylation [60, 61]. The CDK7 inhibitor THZ1 is highly effective in targeting models of c-Myc- ...
16
A Conserved Motif in Region V of the Large Polymerase Proteins of Nonsegmented Negative-Sense RNA Viruses That Is Essential for mRNA Capping
... the mRNA cap plays a key role in controlling polymerase ...the mRNA capping machinery requires specific signals in the RNA (29, 42) and that mRNA cap methylation is accomplished by two ...
11
A temperature-sensitive lesion in the small subunit of the vaccinia virus-encoded mRNA capping enzyme causes a defect in viral telomere resolution.
... PFGE of vaccinia virus DNA from cells infected with wild-type strain IHD-W A or the ts mutant ts9383 B at either the permissive 32°C or the nonpermissive 40°C temperature.. Monolayers of[r] ...
9
Identification of the DNA sequences encoding the large subunit of the mRNA-capping enzyme of vaccinia virus.
... Lanes: 1, no RNA added to the cell-free system; 2, [35S]methionine-labeled polypeptides directed by mRNA hybrid selected by the Hindlll D fragment; 3, 32P-labeled large subunit of the ca[r] ...
9
Virus-Specific mRNA Capping Enzyme Encoded by Hepatitis E Virus
... domain with unknown function, a papain-like protease domain (around 440 to 610 aa), a proline-rich spacer region, an X domain of unknown function, a helicase domain (around 960 to 1,200 aa), and an RNA polymerase domain ...
8
The aging patient: pulp capping in the elderly
... Molecular and cellular changes occur during tooth aging. Odontoblasts, derived from ecto- mesenchymal cells of the dental papilla, are implicated in reactionary dentin formation. Direct or indirect pulp treatments are ...
11
Investigation of Thermal Stress Degradation in Indium-Gallium-Zinc-Oxide TFTs
... Current process integration strategy with ALD Alumina capping improved thermal stability of IGZO TFTs, but is not sufficient to prevent long-channel device degrada- tion. Alternative S/D metal options are being ...
64
Effects of Cu Self Capping and Ta Capping on Nanometer Sized Cu Films Sputter Deposited on β Ta
... between 10 and 30 nm (Ta capping effect). Both these effects were associated with spontaneous rotational motion of the Cu crystallites. For creep deformations (but not for film deposition), such spontaneous ...
7