Multi Locus Sequence Typing (MLST) (5)
Multi locus sequence typing of oenological Saccharomyces cerevisiae strains
... In this work we used MLST analysis for molecular typing of several 89.. cerevisiae wine yeast strains, including isolates from sherry and sparkling wines, 90.[r] ...
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Multi Locus Sequence Typing scheme for Acidithiobacillus caldus strain evaluation and differentiation
... 3. Results 3.1. Comparative genomics-based MLST marker selection pipeline implementation To facilitate and improve the MLST marker selection process, a bioinformatic pipeline was implemented that iden- tifies single copy ...
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A Fast, Accurate, and Automated Workflow for Multi Locus Sequence Typing of Bacterial Isolates
... population. This integrated workflow significantly reduces the time required to analyze and type unknown microbial strains. Data analysis in the current MLST workflow requires 4–5 hours of post-analysis data ...
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Comparison of classical multi locus sequence typing software for next generation sequencing data
... SRST2 is that it takes a 100 base flanking region around the locus from a reference genome, reducing the impact of cov- erage drop off at the ends of the sequences. Additionally, it can assign predicted serovars ...
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Multi Virulence Locus Sequence Typing of Staphylococcus lugdunensis Generates Results Consistent with a Clonal Population Structure and Is Reliable for Epidemiological Typing
... by multi-virulence-locus sequence typing (MVLST), targeting seven known or putative virulence-associated loci (atlL R2 , atlL R3 , hlb, isdJ, SLUG_09050, SLUG_16930, and ...multilocus ...
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Multi locus sequence typing of Escherichia coli isolates with acquired ampC genes and ampC promoter mutations
... Alonso et al. 2016). We therefore set out to investigate whether this was also the case in other parts of the South West of England. Multi-locus sequence typing (MLST) was used to determine ...
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Multi-locus sequence typing (MLST) of C. jejuni infections in the United States Patrick Kwan, PhD
... – Similar to the Dutch model, compares the number of human cases caused by different subtypes with their prevalence in each source, but with additional weighting explicitly added for e[r] ...
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Exploration of genetic diversity of Bacillus spp. from industrial shrimp ponds in Vietnam by multi-locus sequence typing
... Xuan The Le 1,4 , Dung Tien Pham 2 , Tuan Anh Pham 3 , Tung Thanh Tran 2 , Thanh Huu Khuat 2 , Hoa Quang Le 2* and Ut Ngoc Vu 4 Abstract Bacillus is a diverse genus consisting of more than 200 species with extensive ...
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Genotyping of amino acid-producing Corynebacterium glutamicum strains based on multi-locus sequence typing (MLST) scheme
... mechanisms of amino acid hyper-production in these strains. For example, although genome sequences are now available for several glutamate-hyper-producing strains, e.g., SCgG1, SCgG2, and Z188, detail typing and ...
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Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations
... Our MLST results correspond with the previous find- ings that the core genome of P. syringae is highly clonal and displays very little genetic heterogeneity [18]. Each passage consisted of taking 0.1 ml of culture into ...
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Iranian clonal population of Salmonella enterica serovar Enteritidis, char- acterized by multi-locus sequence typing (MLST) method
... molecular typing methods are intended to tackle two different levels of epidemiological prob- lems, which reflect different insights toward solving a local or global epidemiology in different ...
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Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004
... Strains showed nine phage types and 17 pulsed-field profiles with pulsed-field gel electrophoresis. All strains had homologous type 11 sequences without any nucleotide differences in seven housekeeping genes. These MLST ...
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Multi locus sequence typing of Ehrlichia ruminantium strains from geographically diverse origins and collected in Amblyomma variegatum from Uganda
... Conclusions We investigated a recently developed MLST scheme that allows direct genotyping of E. ruminantium by using glo- bal strains from diverse origins and field samples from heartwater-endemic areas in Uganda. As ...
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MetaMLST: multi-locus strain-level bacterial typing from metagenomic samples
... MLST sequence repertoire from public sources removing dupli- cated or chimeric sequences (see ‘Materials and Methods’ section) and we provide the user with the output of this ini- tial ...MLST sequence ...
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Classification of Bartonella Strains Associated with Straw Colored Fruit Bats (Eidolon helvum) across Africa Using a Multi locus Sequence Typing Platform
... applied multi-locus sequence typing, or MLST, to study the genetic differences of straw-colored fruit bat (Eidolon helvum)-associated Bartonella ...
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Pulsed-field gel electrophoresis and multi locus sequence typing for characterizing genotype variability of Yersinia ruckeri isolated from farmed fish in France
... Ségolène Calvez 1,2* , Catherine Fournel 1,2 , Diane-Gaëlle Douet 3 and Patrick Daniel 4 Abstract Yersinia ruckeri is a pathogen that has an impact on aquaculture worldwide. The disease caused by this bacterial species, ...
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Multi-locus sequence type analysis of Shigellas pp. isolates from Tehran, Iran
... many typing methods based on phe- notypic or genotypic properties of bacteria that are selected based on the epidemiological ...the sequence type of each ...molecular typing of bacteria via internet ...
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Multi Virulence Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains of Listeria monocytogenes
... Fragment-based subtyping methods like PFGE have greatly aided the epidemiological investigation of food-borne patho- gens including L. monocytogenes. While PFGE is highly dis- criminatory, it does not always accurately ...
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Resolution of Ambiguous HLA Genotyping in Korean by Multi Group Specific Sequence Based Typing
... HLA-A typing with 10 primer sets for exons 2 and 3, 10 and the AVITA plus as- say expanded this strategy to HLA-B, -C, and -DRB1 typing with the inclusion of exons 1, 4, 5 of HLA-A and -C loci and ...
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Molecular epidemiology of Neisseria gonorrhoeae strains circulating in Indonesia using multi locus variable number tandem repeat analysis (MLVA) and Neisseria gonorrhoeae multi antigen sequence typing (NG MAST) techniques
... b men who have sex with men (including transwomen who did not undergo genital reassignment surgery; c MLVA = Multiple-Locus Variable Number Tandem Repeat (VNTR) Analysis, a cluster was defined as a group of five ...
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