Mutations found during full genome sequencing
A Strategy for Direct Mapping and Identification of Mutations by Whole-Genome Sequencing
... reference genome do not represent an issue for mutation identification using our strategy, as the mutant genomes are compared between each other for identification and removal of background ...
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New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas
... Although fla24 cells assemble full-length flagella at 21°C, IFT81 protein localization is dramatically altered. Instead of localizing at the basal body and along the flagella, IFT81 is concentrated at the proximal ...
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Identification of EMS-Induced Mutations in Drosophila melanogaster by Whole-Genome Sequencing
... next-generation sequencing as a genetics tool, this approach also allows deeper insight into fundamental biological ...have found evi- dence that a significant fraction of noncanonical EMS mutations ...
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Detecting somatic point mutations in cancer genome sequencing data: a comparison of mutation callers
... As demonstrated in the section above, when calling sSNVs, another potential source of false-positives is strand bias. Here, we specifically call an sSNV whose al- ternate alleles all come from one strand a strand-biased ...
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Spatial Vulnerabilities of the Escherichia coli Genome to Spontaneous Mutations Revealed with Improved Duplex Sequencing
... spontaneous mutations by next-generation sequencing technology has attracted extensive attention lately due to the fundamental roles of spontaneous mutations in evolution and pathological ...the ...
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Full genome re sequencing reveals a novel circadian clock mutation in Arabidopsis
... 8 genome release, with information extracted from TIGR xml formatted files cross-referenced with FASTA formatted sequence ...changing) mutations, or as causing the creation or deletion of stop ...entire ...
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Genotype and Subtype Independent Full Genome Sequencing Assay for Hepatitis C Virus
... predominantly found in the E1/E2 and NS5A regions in most patients, independent of geno- ...HCV genome are associated with lower treatment response due to an increased likelihood of the presence of preex- ...
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Genomic Context of Azole Resistance Mutations in Aspergillus fumigatus Determined Using Whole Genome Sequencing
... Our demonstration of high levels of SNP diversity and a pan- mictic population structure within the United Kingdom and Netherlands is testament to the ability of this highly aerosolized (airborne) fungus to passively ...
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Whole genome sequencing reveals novel mutations causing autosomal dominant inherited macular degeneration
... had full ophthalmic examinations and color fundus photography, spectral- domain ocular coherence tomography and scanning laser ...suspected mutations in C1QTNF5 and TIMP3 followed by whole genome ...
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Genes found in partial sequencing of Phytophthora cinnamomi
... expressed during the different stages and types of infections is of essential importance in understanding the biological and physiological responses of those fungal pathogens to their environment (Choupina et ...
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Whole-genome sequencing of bladder cancers reveals somatic CDKN1A mutations and clinicopathological associations with mutation burden
... of genome-wide deep sequencing allowed us to perform extensive work on structural variations, including copy number changes and ...were found, several focal regions of deletion and gain were ...was ...
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Whole genome sequencing reveals that recurrent MYD88 and CXCR4 mutations underlie the genomic landscape of Waldenström's macroglobulinemia
... A full complete list of significant copy number windows is shown in Appendix ...were found outside of chromosome 6 with a median length of 100Kb (range 100Kb- 300Kb), of which 126 of 134 (94%) were ...
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Genome sequencing reveals fine scale diversification and reticulation history during speciation in Sus
... with mutations consistent with incom- plete lineage sorting: those where P1 and P3 (BABA) or P2 and P3 (ABBA) share the derived allele (B; assuming ancestral state, A, in the ...
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Crowdfunded whole-genome sequencing of the celebrity cat Lil BUB identifies causal mutations for her osteopetrosis and polydactyly
... Lil BUB was found as a feral kitten that was abandoned by her mother in rural Indiana, USA and was subsequently hand-reared. She was born with several malformations, mainly related to skeletal growth and ...
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Efficient isolation on Vero.DogSLAMtag cells and full genome characterization of Dolphin Morbillivirus (DMV) by next generation sequencing
... (2011) found that the 2 (A → V) mutation was exclusive of the DMV_Gme/2007 sequence suggesting that it might be associated to DMV adaptation to the long-finned pilot whale ...
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Single genome sequencing of near full-length HIV-1 RNA using a limiting dilution approach
... dilution sequencing using either RNA or cDNA as starting material and were surprised to see that despite the long am- pli fication length, both are feasible and equally ...where sequencing of individual ...
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Direct multiplexed whole genome sequencing of respiratory tract samples reveals full viral genomic information
... 3.2. Sequencing of clinical samples From all samples, sufficient amounts of DNA and RNA were obtained to perform whole-genome sequencing for the detection of DNA viruses, or whole-transcriptome ...
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Targeted full-genome amplification and sequencing of dengue virus types 1–4 from South America
... DENV genome copies using qPCR ( McAvin et ...DENV-specific genome copies across ...amplicon sequencing strategy, the researcher is able to focus on samples that have a high probability of success and ...
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Twenty years of bacterial genome sequencing
... throughput sequencing has seen this approach move ever closer to routine clinical and public health ...benchtop sequencing, open data release and social media catalysed the analysis of genomes during ...
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Efficient Mapping and Cloning of Mutations in Zebrafish by Low-Coverage Whole-Genome Sequencing
... Whole-genome sequencing has recently emerged as a fast and ef fi cient approach for identifying mutations in nonvertebrate model ...large genome and lack of fully inbred ...detecting ...
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