Mycobacterium bovis BCG
Evaluation of the Immunogenicity of Mycobacterium bovis BCG Delivered by Aerosol to the Lungs of Macaques
... the Mycobacterium bovis BCG vaccine provides limited protection, whereas aerosol delivery has been shown to enhance efficacy in experimental ...aerosol-delivered BCG vaccine. Aerosol delivery ...
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Humoral Immune Responses of White-Tailed Deer (Odocoileus virginianus) to Mycobacterium bovis BCG Vaccination and Experimental Challenge with M. bovis
... animals). Mycobacterium bovis BCG Danish strain 1331 in culture and in lipid-formulated pellets was prepared by Immune Solutions ...M. bovis strain 9839 (NADC designation) by the ...
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Extracellular and Cytosolic Iron Superoxide Dismutase from Mycobacterium bovis BCG
... Two forms of iron superoxide dismutase (SOD) were purified from cell extract (CE) and culture filtrate (CF) of Mycobacterium bovis BCG, respectively. The molecular weight of both enzymes was ...
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Intranasal Administration of Mycobacterium bovis BCG Induces Superior Protection against Aerosol Infection with Mycobacterium tuberculosis in Mice
... bacilli, Mycobacterium tuberculosis remains one of the most successful human pathogens and a major global public health ...uated Mycobacterium bovis BCG is the only TB vaccine approved for ...
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Arylamine N acetyltransferase is required for synthesis of mycolic acids and complex lipids in Mycobacterium bovis BCG and represents a novel drug target
... and Mycobacterium bovis BCG and the gene product is active ...M. bovis (23) have been sequenced. M. bovis is a member of the ...M. bovis BCG is an attenuated M. ...
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Inhibition of Mycobacterial Growth In Vitro following Primary but Not Secondary Vaccination with Mycobacterium bovis BCG
... the Mycobacterium bovis BCG vaccine, there are more than 9 million new cases of tuberculosis (TB) every year, and there is an urgent need for better TB ...with BCG (P < 0.05). Mycobacterial ...
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Robust Immunity to an Auxotrophic Mycobacterium bovis BCG-VLP Prime-Boost HIV Vaccine Candidate in a Nonhuman Primate Model
... of Mycobacterium bovis BCG expressing human immuno- deficiency virus type 1 (HIV-1) subtype C Gag (rBCGpan-Gag) efficiently primes the mouse immune system for a boost with a recombinant modified ...
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Influence of Mycobacterium bovis BCG Vaccination on Cellular Immune Response of Guinea Pigs Challenged with Mycobacterium tuberculosis
... for BCG- vaccinated guinea pigs showed increased expression of MHC class II on MR-1 ⫹ macrophages compared to that for the control ...and BCG-vaccinated animals infected with ...the BCG-vaccinated ...
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Influence of Advanced Age on Mycobacterium bovis BCG Vaccination in Guinea Pigs Aerogenically Infected with Mycobacterium tuberculosis
... infants. BCG provides efficient pro- tection against severe and disseminated TB, such as tubercu- losis meningitis and miliary TB, in children (33, 34, ...that BCG vaccination had long-term efficacy for ...
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Development of Diagnostic Reagents To Differentiate between Mycobacterium bovis BCG Vaccination and M. bovis Infection in Cattle
... ESAT-6 and MPB64, both constituents of the diagnostic cocktails used in this study, have been tested previously in cattle. ESAT-6 has been shown to induce IFN-␥ production in 85 to 90% of the field reactors tested in ...
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Differentiation between Mycobacterium bovis BCG-Vaccinated and M. bovis-Infected Cattle by Using Recombinant Mycobacterial Antigens
... M. bovis anti- gens in cattle ...between BCG and virulent strains of the tuberculosis ...M. bovis but not in BCG ...M. bovis infection in cattle ...M. bovis, and some, but not ...
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A novel method for monitoring Mycobacterium bovis BCG trafficking with recombinant BCG expressing green fluorescent protein.
... wild-type BCG by both human bladder tumor cell line T-24 and mouse bladder tumor cell line MBT-2 ...wild-type BCG phagocytosis by both T-24 and MBT-2 cells in that maximal internalization was observed at 16 ...
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Deletion of nuoG from the Vaccine Candidate Mycobacterium bovis BCG ΔureC::hly Improves Protection against Tuberculosis
... precipitation was performed using isopropanol, ammonium acetate (Am- bion AM9070G), and glycogen (Ambion AM9510), and pellets were washed with 70% ethanol and resuspended in RNase-free water on ice. The concentrations ...
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Reactivation of immune responses against Mycobacterium tuberculosis by boosting with the CpG oligomer in aged mice primarily vaccinated with Mycobacterium bovis BCG
... the BCG vaccinated mice (Figure 3), and CD4 + memory type T cells were strongly induced by the boosting (Table 1, CD4 + CD44 hi CD62L high in 90-week old mice: after boosting; ...
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Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults
... in BCG immunogenicity has been ascribed to differences in preexisting antimycobacterial responses in settings where disease is endemic versus nonendemic (36, 37), the pres- ence of helminth infections (38), ...
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Use of Antigen-Specific Interleukin-2 To Differentiate between Cattle Vaccinated with Mycobacterium bovis BCG and Cattle Infected with M. bovis
... with Mycobacterium bovis and in cattle vaccinated with Myco- bacterium bovis BCG and then experimentally challenged with pathogenic ...M. bovis produced quantifiable levels of ...
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Stable Expression of Lentiviral Antigens by Quality-Controlled Recombinant Mycobacterium bovis BCG Vectors
... of BCG was cleared after 7 weeks of growth in mice (53) suggested that BCG leucine auxo- trophs could not adequately scavenge for leucine in vivo, and con- struction of a plasmid with complementing genes ...
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Use of Antigen-Specific Interleukin-2 To Differentiate between Cattle Vaccinated with Mycobacterium bovis BCG and Cattle Infected with M. bovis
... in BCG [1]) showed an increase in the sensitivity of detec- tion of Mycobacterium bovis-infected cattle without compromis- ing specificity ...
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Efficient Activation of Human T Cells of Both CD4 and CD8 Subsets by Urease-Deficient Recombinant Mycobacterium bovis BCG That Produced a Heat Shock Protein 70-M. tuberculosis-Derived Major Membrane Protein II Fusion Protein
... by BCG- ...produced BCG- DHTM secreted the fusion protein composed of ...of BCG-DHTM (Fig. 2). When either BCG-261H or BCG-DHTM was used to infect DCs and was used as a stimulator, ...
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Safety and Immunogenicity of the Recombinant Mycobacterium bovis BCG Vaccine VPM1002 in HIV-Unexposed Newborn Infants in South Africa
... Immunological analyses. (i) Whole-blood assays. Assessment of the immunogenicity of VPM1002 and BCG was performed using a well-established assay (66). In brief, whole blood from vaccinated infants was stimulated ...
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