Mycolic acid
Rapid, Standardized Method for Determination of Mycobacterium tuberculosis Drug Susceptibility by Use of Mycolic Acid Analysis
... measuring mycolic acid levels by high-performance liquid chromatogra- phy (HPLC) (10, ...21). Mycolic acids are high-molecular-weight ␣ -alkyl,  -hydroxy fatty acids containing an ␣ branch of 20 to ...
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Keto Mycolic Acid Dependent Pellicle Formation Confers Tolerance to Drug Sensitive Mycobacterium tuberculosis
... Growth as pellicles requires a high degree of self-organization owing to the lack of a solid surface on which growth can be initi- ated. Scanning electron microscopy (SEM) of M. tuberculosis pel- licles following ...
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Rifampin- or Capreomycin-Induced Remodeling of the Mycobacterium smegmatis Mycolic Acid Layer Is Mitigated in Synergistic Combinations with Cationic Antimicrobial Peptides
... in mycolic acid is consistent with fatty acids being diverted for use as a carbon source; notably, in contrast with the results for rifampin challenge, no significant changes in individual metabolite levels ...
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A mycolic acid–specific CD1 restricted T cell population contributes to acute and memory immune responses in human tuberculosis infection
... Current tuberculosis (TB) vaccine strategies are largely aimed at activating conventional T cell responses to mycobacterial protein antigens. However, the lipid-rich cell wall of Mycobacterium tuberculosis (M. ...
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Rapid identification of mycolic acid patterns of mycobacteria by high performance liquid chromatography using pattern recognition software and a Mycobacterium library
... Rapid Identification of Mycolic Acid Patterns of Mycobacteria by High-Performance Liquid Chromatography Using Pattern Recognition Software and a Mycobacterium Library SUZANNE E.. SCOTT R[r] ...
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Mycolic acid analysis for clinical identification of Mycobacterium avium and related mycobacteria
... Finally, 12 clinical isolates identified as MAI complex strains had negative test results for photochromogenicity and for niacin and urease production but formed the mycolic acid pattern[r] ...
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Two Accessory Proteins Govern MmpL3 Mycolic Acid Transport in Mycobacteria
... TtfA, a protein with no predicted domains of known function, is an essential component of the mycolic acid transport machinery. We have defined the essential portions of TtfA, amino acids 1 to 205 that ...
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Analysis of mycolic acid cleavage products and cellular fatty acids of Mycobacterium species by capillary gas chromatography
... Long-chain fatty acid methyl ester cleavage products and constituent fatty acids and alcohols if present of representative Mycobacterium species were determined by capillary GLC after gr[r] ...
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Identification of mycolic acid forms using surface-enhanced Raman scattering as a fast detection method for tuberculosis
... Purpose: Conventional TB detection methods are either too slow as it takes a few weeks for diagnosis or they lack the specificity and accuracy. Thus the objective of this study was to develop a fast and efficient ...
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Gas chromatographic analysis of mycolic acid cleavage products in mycobacteria
... When the samples were analyzed at high injector temperatures of 300 to 350°C, characteristic thermal cleavage products from mycolic acids, C220, C240, or C260 fatty acid methyl esters, w[r] ...
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Arylamine N acetyltransferase is required for synthesis of mycolic acids and complex lipids in Mycobacterium bovis BCG and represents a novel drug target
... mycobacteria. Mycolic acid biosynthesis is inhibited by isoniazid, a key frontline antitubercular drug that is inactivated by mycobacterial and human arylamine N -acetyltransferase ...of mycolic ...
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Adaptive response of Rhodococcus opacus PWD4 to salt and phenolic stress on the level of mycolic acids
... the mycolic acid composition, we investigated the composition of mycolic acids during the response both to osmotic stress caused by NaCl and to 4-chlorophenol in Rhodococcus opacus ...between ...
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Synthesis of N (2 nitrophenyl) pyrazine 2 carboxamide and N (4 nitrophenyl) pyrazine 2 carboxamide and evaluation of their antimycobacterial properties using in vitro and in silico techniques
... Quinolinic acid phosphoribosyltransferase (QAPRTase), a key enzyme in the de novo biosynthesis of ...that mycolic acid cyclopropane synthases (PcaA, CmaA1, and CmaA2) play a vital role in ...
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Comparison of Methods for Identification ofMycobacterium abscessus and M chelonae Isolates
... It is clinically important to correctly identify these organisms since they cause infections requiring different treatment regi- mens (R. J. Wallace, Jr., B. A. Brown, D. E. Griffith, Letter, Pediatr. Infect. Dis. J. ...
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Trans cyclopropanation of mycolic acids on trehalose dimycolate suppresses Mycobacterium tuberculosis –induced inflammation and virulence
... the mycolic acids on trehalose dimycolate (TDM) mediated by proximal cyclopropane synthase of a mycolates (pcaA), a proinflammatory lipid modification during early ...of mycolic acid cyclopropane ...
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Improved rapid identification of mycobacteria by combining solid phase extraction with high performance liquid chromatography analysis of BACTEC cultures
... single-cluster mycolic acid HPLC ...ficient mycolic acids were detected to permit identification of the culture by ...diagnostic mycolic acids were concentrated to yield a chromatogram us- ...
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Separation among Species of Mycobacterium terrae Complex by Lipid Analyses: Comparison with Biochemical Tests and 16S rRNA Sequencing
... Identification to the species level of the strains belonging to the Mycobacterium terrae complex, i.e., M. terrae sensu stricto, M. nonchromogenicum, and M. triviale, has been regarded as unnecessary, due to the ...
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TWEEN 80 ALTERS THE PRODUCTION RATIO OF PHARMACEUTICALLY IMPORTANT STEROID INTERMEDIATES, 4 AD AND ADD DURING BIOTRANSFORMATION OF SOY STEROL BY MYCOBACTERIUM SP NRRL B 3805
... and mycolic acid contents of Mycobacterium ...in mycolic acid was ...wall mycolic acid content could be correlated with increased ADD ...
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Mycobacterium heckeshornense sp nov , a New Pathogenic Slowly Growing Mycobacterium sp Causing Cavitary Lung Disease in an Immunocompetent Patient
... Fatty acid methyl esters were obtained by saponi- fication, methylation, and extraction, separated by gas-liquid chromatography (GLC), and determined by using the Microbial Identification System standard software ...
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Financial Intermediation and Economic Growth: Bank Credit Maturity and Its Determinants
... envelope, mycolic acids content and the composition of the cell envelope were ...for mycolic acid production (Candida albicans C30) ...the mycolic acid analysis, therefore it was not ...
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