Next-generation whole genome sequencing
Ultrasensitive single genome sequencing: accurate, targeted, next generation sequencing of HIV 1 RNA
... Although next generation sequencing (NGS) offers the potential for studying virus populations in unprecedented depth, PCR error, amplification bias and recombination during library construction have ...
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Scalable and reproducible genome analysis in the age of next-generation genome sequencing
... A genome sequence for Polistes dominula, the best-studied member of the model genus Polistes, greatly enhances our power to study the genetics of social behavior via comparative genomic and transcriptomic ...
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Quantifying next generation sequencing sample pre-processing bias in HIV-1 complete genome sequencing
... and sequencing technologies were quickly adopted by HIV researchers in order to broaden the understanding of the clinical importance of minor drug-resistant ...multiple genome regions for drug resistance ...
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Biological Characterization and Next-Generation Genome Sequencing of the Unclassified Cotia Virus SPAn232 (Poxviridae)
... Beyond these conflicting results, no information regarding the COTV replicative cycle is available. In this work, we have analyzed the biology and genomics of COTV. Our results show that cul- tured cells infected with ...
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Next generation sequencing
... parallel sequencing methods overcome the limited scalability of traditional Sanger sequencing by either creating micro-reactors and/or attach- ing the DNA molecules to be sequenced to solid surfaces or ...
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Whole genome sequencing of aquaculture species
... Sanger sequencing technology for the rst phase, and perhaps next-generation sequencing technologies when appropriate for later phases of the ...salmon genome that is believed to be ...
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Whole-Exome/Genome Sequencing and Genomics
... Whole-Exome/Genome Sequencing and Genomics abstract As medical genetics has progressed from a descriptive entity to one focused on the functional relationship between genes and clinical dis- orders, ...
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Next generation sequencing (NGS)
... is that it can be integrated with a read coverage plot (Fig. 1a), which can reveal regions of unexpectedly high coverage often indicative of similar sequences that were erroneously collapsed by the assembler into one. ...
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The prediction of HLA genotypes from next generation sequencing and genome scan data
... for whole genome sequencing ...for whole exome sequencing using capture technology, this may be violated by the specific bait designs used for the HLA genes being ...
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Next generation sequencing reveals the antibiotic resistant variants in the genome of Pseudomonas aeruginosa
... addition, genome sequencing facilitates high throughput screening for specific mutations within antibiotic resistant genes compared to the susceptible and/or reference strain of the same bacterial ...
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Whole-genome sequencing in an autism multiplex family
... Therefore, we attempted to address this problem by performing a pilot sequencing analysis on patients from multiplex families. We selected a large two-generation family, with parents and eight children, two ...
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Analysis of Somatic Alterations in Cancer Genome: From SNP Arrays to Next Generation Sequencing
... cancer genome (Podlaha et al., 2012). Studying the cancer genome is important as it may unravel the key genomic events or some particular genomic features, which could shed light on tumor biology and have ...
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Genome analysis pipeline for next-generation sequencing
... There were two main aims in this thesis. First, to create a pipeline which can be used to analyse genomic sequencing. Second, to use the pipeline to compare whole human exome capture methods from two ...
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Beyond the whole genome consensus: Unravelling of PRRSV phylogenomics using next generation sequencing technologies
... For example, such technology could help shed light on the genetic evolution of PRRSV during immune selection (Evans et al., 2014). However, further developments are required for the field to gain even more from this NGS ...
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Next-Generation Sequencing and Genome Editing in Plant Virology
... RNA sequencing for plant viruses and ...the whole genome, have been sequenced for discovery of novel pathogens, as well as for pathogen detection and identification, replication, ecology, ...
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Preparing whole genome human mitochondrial DNA libraries for next generation sequencing using Illumina Nextera XT
... Sanger sequencing: sequence chemistry, read length, sequence time and sequence accuracy (Loman et ...Sanger sequencing, which can generate up to 1000 bases of sequencing data (Shendure and Ji ...for ...
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Diagnosis of mitochondrial disorders by concomitant next-generation sequencing of the exome and mitochondrial genome
... exome sequencing yielded a molecular diagnosis after none were identi fied using conventional ...Although whole exome sequencing is not normally needed to detect this speci fic variant, the fact that ...
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Statistical Methods and Analysis in Genome Wide Association Studies and Next- Generation Sequencing.
... 103 Our GWAS in Chapter 2 identified a novel gene and a potentially interesting pathway. To detect more genetic variants with small effect size or low minor allele frequency, more samples are needed. A common approach is ...
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Determining the quality and complexity of next generation sequencing data without a reference genome
... of whole genome ...in sequencing depth between samples to expose outliers and problematic samples and that, like SGA, it does not rely on prior ...of sequencing data, estimate the ...
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Next-generation whole-exome sequencing contribution to identification of rare autosomal recessive diseases
... by whole-ex- ome sequencing, autoimmune lymphoproliferative syndrome and aplastic anemia could be ...of whole-exome se- quencing and genome-wide linkage analysis it was shown that ...
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