Non-viral gene delivery
Hyperbranched poly(ethylenimine co oxazoline) by thiol–yne chemistry for non viral gene delivery : investigating the role of polymer architecture
... equivalent. Delivery of pDNA encoding for GFP showed that poly(ethylenimine-co-oxazoline) copolymers with high percentages of ethylenimine units were have transfection efficiencies slightly lower than the ...
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Cationic lipids and investigations into non-viral gene delivery systems
... The interaction of cationic liposomes with negatively charged vesicles and biological membranes was investigated by Stamatatos et al}^ The fusion of liposomes, comprised of PE lipids and DOTAP, with negatively charged ...
267
Non viral gene delivery systems for tissue repair and regeneration
... The targeting design of an nVV is a way to limit reac- tion in local tissue. Some peptides have the ability to demonstrate a target delivery. For example, ATS-9R, an adipocyte-targeting sequence, was combined with ...
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NANO-STRUCTURES FOR NON-VIRAL GENE DELIVERY FOR CANCER THERAPY: A REVIEW
... the gene therapy to identify a malfunctioning gene and supply the patient with proper functioning copies of that ...of gene therapy is to introduce therapeutic material into the diseased the cell, ...
7
Reprogramming fibroblasts to pluripotency using arginine-terminated polyamidoamine nanoparticles based non-viral gene delivery system
... the delivery of defined transcription factor (Oct4, Sox2, Klf4, and ...genome-integrating viral vec- ...the delivery of a single plasmid construct carrying OSKM ...
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Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System
... while non-viral gene delivery techniques work toward efficiency of DNA delivery, they generally prove poor in the delivery of pDNA vectors to the nuclear com- ...of ...
13
Mesenchymal stem cell fate following non viral gene transfection strongly depends on the choice of delivery vector
... the delivery of regulatory genes is a promising strategy in tissue engineering ...effective gene delivery is the choice of gene carrier. Non-viral delivery vectors have ...
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Improved osteogenic vector for non-viral gene therapy
... of gene therapy encoding an exogenous gene on a plasmid (Feichtinger et ...Therefore, delivery of up to mg dosages at once are required to provide a sufficient ...alternative, gene therapy ...
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Meyer, Martin (2009): Dynamic endosomolytic polymer conjugates for pDNA and siRNA delivery. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... PEI is one of the most frequently used polycations for non-viral gene delivery. PEI polymers can be synthesized with a branched or a linear structure and are available in a wide range of ...
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A sight on the current nanoparticle based gene delivery vectors
... ful non-viral gene delivery ...good gene delivery properties especially when incorporated in the colloidal particulate systems ...other viral and non-viral ...
9
An investigation of in vitro-assembled chromatin as a novel vehicle for gene delivery
... BARRIERS TO GENE DELIVERY Whether viral or non-viral gene delivery systems are used, the therapeutic plasmid faces a challenging route to the nucleus that necessitates traversing a serie[r] ...
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Recent advances in the development of gene delivery systems
... for gene delivery system ...for gene delivery in vivo to the liver has been developed ...poly-L-lysine-based gene delivery systems have been reported ...targeted gene ...
7
Constitutive and inducible co-expression systems for non-viral osteoinductive gene therapy
... enable non-invasive gene expression monitoring, we incorporated an internal luciferase control ...BMP2 gene delivery, where 4 injections of 125 µg BMP2 DNA led to 57 % and 8 injections of ...
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Supramolecular Assemblies of Peptides or Nucleopeptides for Gene Delivery
... for gene delivery. Realizing the intrinsic weakness of viral vectors, such as immunogenicity, researchers have focused on the development of non-viral vectors, especially virus-like ...
10
Abstract
... Successful gene trans− fer depends on several factors, including reagent/DNA complex formation, entry into the cell, cytosolic transport, release of DNA from the complex, and DNA uptake into the nucleus ...improve ...
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Targeted, systemic non viral delivery of small interfering RNA in vivo
... of gene function and the identification of drug targets in mammalian ...a non-specific interferon pathway (through interaction with the protein kinase PKR 1 and 2’,5’-oligoadenylate synthetase 2 ) rather ...
139
Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers
... intracellular delivery of K8 antibody to 67% of HeLa ...regulator gene (CFTR) and its associated protein. Delivery of the active K8 antibody to these cells has demonstrated to prevent disease ...
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Non-viral siRNA delivery into the mouse retina in vivo
... The technology of RNA interference (RNAi) offers the perspective for selective and on demand silencing of gene expression. One of the critical factors that limit the experimental and therapeutic application of ...
5
Selective gene silencing by viral delivery of short hairpin RNA
... experimental gene therapy and 25% of clinical gene therapy trials currently underway are using adenovirus ...medium-sized, non-enveloped viruses with a nucleocapsid and a linear dsDNA gen- ...and ...
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Functional Nanostructures for Effective Delivery of Small Interfering RNA Therapeutics
... target-specific gene silencing via RNA interference ...targeted gene expression gives new hope to gene therapy as a treatment for cancers and genetic ...the delivery of siRNA therapeutics into ...
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